Concepts of Biology
1st Edition
ISBN: 9781938168116
Author: Samantha Fowler, Rebecca Roush, James Wise
Publisher: OpenStax College
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Textbook Question
Chapter 10, Problem 2RQ
n gel electrophoresis of DNA, the different bands in the final gel form because the DNA molecules _______.
a. are from different organisms
b. have different lengths
c. have different
d. have different genes
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In gel electrophoresis of DNA, the different bands in the final gel form because the DNA molecules ________.a. are from different organismsb. have different lengthsc. have different nucleotide compositionsd. have different genes
DNA fragments that are 500 bp, 1000 bp, and 2000 bp in length are separated by gel electrophoresis. Which fragment will migrate farthest in the gel?
a. The 2000-bp fragment
b. The 1000-bp fragment
c. The 500-bp fragment
d. All will migrate equal distances.
Electrophoresis separates fragments of DNA according to_____ . a. sequence b. length c. species
Chapter 10 Solutions
Concepts of Biology
Ch. 10 - Figure 10.7 Why was Dolly a Finn-Dorset and not a...Ch. 10 - n gel electrophoresis of DNA, the different bands...Ch. 10 - In the reproductive cloning of an animal, the...Ch. 10 - What carries a gene from one organism into a...Ch. 10 - What is a genetically modified organism (GMO)? a....Ch. 10 - What is the role of Agrobacterium tumefaciens in...Ch. 10 - What is the most challenging issue facing genome...Ch. 10 - Genomics can be used in agriculture to: a....Ch. 10 - What kind of diseases are studied using...Ch. 10 - What is the purpose and benefit of the polymerase...
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- Match the outcomes on the left with the laboratory steps on the right. ___ Harvest the cells A. Add salt ___ Dissolve cell membranes B. Add protease, incubate at 50°C ___ Precipitate the DNA C. Mix in a detergent solution ___ Break down proteins D. Layer cold alcohol over cell extract ___ Make DNA less soluble in water E. Gently chew the insides of your mouth, then rinse vigorously with waterarrow_forwardWe want to extract DNA from this culture of bacteria suspended in Media the bacteria cells are floating in the liquid. Our first step is to centrifuge our sample. Do we keep the supernatant or the pellet and why? A. Supernatant because it contains Calais or nutrients B. Pellet because it contained only DNS and the supernatant contains the rest of the cell part C. Supernatant because it contains only dna and the pellet contains the rest of the cell parts D. Pellet because it contains the parts of the bacterial cell with the plasmid DNAarrow_forwarda. Pfu Polymerase b.dNTPs c.Buffer Match each component above to the correct function(s) listed below. Write your selection(s) for each component. You may have more than one answer for each. 1. unwinds DNA 2. synthesizes new DNA strands 3. enzymatically catalyzes Quikchange 4. nucleotide source for new DNA strands 5. Energy source for reaction(s) 6. Repairs errors in base pair matching 7. Maintains pH and salt levels 8. Creates polymer chainsarrow_forward
- Gel electrophoresis separates the DNA fragments according to ____. a. their type b. their stage in replication c. the orientation of the nucleotides in the fragment d. their size e. their ability to bind to the gelarrow_forwardNucleotide excision repair A. recognizes and repairs thymine dimers and other damaged bases in DNA B. corrects errors in nucleotide excision C. recognizes and removes mismatched bases after DNA synthesis is completed D. removes nucleotides that are incorrectly incorporated during DNA synthesisarrow_forwardFranklin and Wilkins made diffraction patterns of DNA using ___. A. X-ray crystallography B. microscopes C. electron microscopy D. X-ray microscopyarrow_forward
- We use thermostable polymerase in Polymerase chain reaction as we use ______ to unwind DNA strands A. helicase B. denaturants C. none of the above D. heatarrow_forwardA biochemist isolates, purifies and combines ina test tube a variety of molecules needed for DNA replication. When she adds some DNA to the mixture, replication occurs, but each DNA molecule consists of a normal strand paired with numerous segments of DNA a few hundred nucleotides long. What has she probably left out of the mixture? a.DNA polymerase b. DNA ligase c. Okajaki fragments d. primasearrow_forwardDuring replication, proofreading is accomplished by _____ and removes the incorrect base ____ a. DNA pol III 5’-3’ b. DNA pol I 3’-5’ c. DNA pol III 3’-5’ d. DNA pol I 5’-3’arrow_forward
- Which of the following strands of DNA (assuming it was bound to its complementary strand to create a double-helix structure) would be the most difficult to break apart? O a. 5'- CCGCCAAGGCAT - 3' O b. 5' - CAATTAGTTAAC - 3' O C. 5'- AAATTGATATAT - 3' d. 5'- AATGCGCCGATA - 3' e. 5'- CCAGGCCGTGGA - 3'arrow_forwardWhich DNA polymerase in prokaryotes is involved in the replacement of primers with deoxynucleotides? a.DNA-Pol I b.DNA-Pol II c.DNA-Pol Ill d.DNA-Pol IVarrow_forwardThe enzyme ________ separates the TWO DNA strands of the double helix. a. helicase b. ligase c. polymerasearrow_forward
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