(a) Interpretation: The values of k c a t C O 2 / K M C O 2 and k c a t O 2 / K M O 2 as s- 1 M-1 should be determined. Concept introduction: If the concentration of enzyme is constant and the substrate concentration is gradually increased, the reaction velocity also increases up to a maximum and after that it will remain constant even though the substrate concentration increases further. The Michael is constant, K M is the substrate concentration at half of the maximum velocity. The smaller the K M of an enzyme, the smaller the amount of substrate required to saturate the enzyme. k cat refers to the turn over number which is defined as the number of substrate molecules that is converted to product by each enzyme site per unit time.

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Answer 1

Question

Chapter 20, Problem 43P

Interpretation Introduction

(a)

Interpretation:

The values of kcatCO2/KMCO2 and kcatO2/KMO2 as s-¹M-1 should be determined.

Concept introduction:

If the concentration of enzyme is constant and the substrate concentration is gradually increased, the reaction velocity also increases up to a maximum and after that it will remain constant even though the substrate concentration increases further.

The Michael is constant, K_M is the substrate concentration at half of the maximum velocity. The smaller the K_M of an enzyme, the smaller the amount of substrate required to saturate the enzyme.

k_cat refers to the turn over number which is defined as the number of substrate molecules that is converted to product by each enzyme site per unit time.

Interpretation Introduction

(b)

Interpretation:

The reason for the occurrence of oxygenation reaction should be determined.

Concept introduction:

The ratio between turn over number and Michael is constant; kcatKM, is known as specificity constant. It is a measure of the efficiency of the enzyme on two competing substrates.

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Enzyme X exhibits maximum activity at pH- 6.3. X shows a fairly sharp decrease in its activity when the pH goes much lower than 5.8. One likely interpretation of this pH activity is that: a Glu residue on the enzyme is involved in the reaction. O the enzyme uses NADH has a cofactor the enzyme uses coenzyme A has a cofactor O a Tyr residue on the enzyme is involved in the reaction. O a His residue on the enzyme is involved in the reaction

Each plot has its own limitations. Match the plot with the correct statement on limitations. 1.Hanes-Woolf 2.Michaelis Menten 3.Lineweaver-Burk limitations: A. This plot appears to allow for more accurate calculation of these parameters, but is prone to error, as the y-axis takes the reciprocal of the rate of reaction –this increases any small errors in measurement at low substrate concentration, where they are most likely to occur. B.The advantage of this plot is that it does not not overemphasizing the data obtained at low [S]. The disadvantage is that any inaccurate measures of substrate concentration will be exaggerated as [S] is used on both axes. C.It is difficult to obtain a meaningful value of Km from this plot because of the high substrate concentrations needed to reach Vmax. Vmax has to be estimated by extrapolation- which is partly subjective and introduces error. D.The advantage of this plot is that it overcomes uneven spacing of points, and undue weight of a…

ch Select all statements that are correct. Note there might be more than 1 correct statement. From the Lineweaver-Burk plot the equilibrium constant (Keq) can be obtained The Lineweaver-Burk plot gives a more accurate prediction for Vmax than the Michaelis- Menten plot The Lineweaver-Burk plot assumes that products and reactants are present at equal concentrations during the entire time of the reaction The Lineweaver-Burk plot shows velocity of reaction vs substrate concentration The Lineweaver-Burk plot shows 1/velocity of reaction vs 1/substrate concentration O 20°C D) // E

Answer 2

Question

Chapter 20, Problem 43P

Interpretation Introduction

(a)

Interpretation:

The values of kcatCO2/KMCO2 and kcatO2/KMO2 as s-¹M-1 should be determined.

Concept introduction:

If the concentration of enzyme is constant and the substrate concentration is gradually increased, the reaction velocity also increases up to a maximum and after that it will remain constant even though the substrate concentration increases further.

The Michael is constant, K_M is the substrate concentration at half of the maximum velocity. The smaller the K_M of an enzyme, the smaller the amount of substrate required to saturate the enzyme.

k_cat refers to the turn over number which is defined as the number of substrate molecules that is converted to product by each enzyme site per unit time.

Interpretation Introduction

(b)

Interpretation:

The reason for the occurrence of oxygenation reaction should be determined.

Concept introduction:

The ratio between turn over number and Michael is constant; kcatKM, is known as specificity constant. It is a measure of the efficiency of the enzyme on two competing substrates.

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Answer 3

Question

Chapter 20, Problem 43P

Interpretation Introduction

(a)

Interpretation:

The values of kcatCO2/KMCO2 and kcatO2/KMO2 as s-¹M-1 should be determined.

Concept introduction:

If the concentration of enzyme is constant and the substrate concentration is gradually increased, the reaction velocity also increases up to a maximum and after that it will remain constant even though the substrate concentration increases further.

The Michael is constant, K_M is the substrate concentration at half of the maximum velocity. The smaller the K_M of an enzyme, the smaller the amount of substrate required to saturate the enzyme.

k_cat refers to the turn over number which is defined as the number of substrate molecules that is converted to product by each enzyme site per unit time.

Interpretation Introduction

(b)

Interpretation:

The reason for the occurrence of oxygenation reaction should be determined.

Concept introduction:

The ratio between turn over number and Michael is constant; kcatKM, is known as specificity constant. It is a measure of the efficiency of the enzyme on two competing substrates.

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Answer 4

Question

Chapter 20, Problem 43P

Interpretation Introduction

(a)

Interpretation:

The values of kcatCO2/KMCO2 and kcatO2/KMO2 as s-¹M-1 should be determined.

Concept introduction:

If the concentration of enzyme is constant and the substrate concentration is gradually increased, the reaction velocity also increases up to a maximum and after that it will remain constant even though the substrate concentration increases further.

The Michael is constant, K_M is the substrate concentration at half of the maximum velocity. The smaller the K_M of an enzyme, the smaller the amount of substrate required to saturate the enzyme.

k_cat refers to the turn over number which is defined as the number of substrate molecules that is converted to product by each enzyme site per unit time.

Interpretation Introduction

(b)

Interpretation:

The reason for the occurrence of oxygenation reaction should be determined.

Concept introduction:

The ratio between turn over number and Michael is constant; kcatKM, is known as specificity constant. It is a measure of the efficiency of the enzyme on two competing substrates.

Expert Solution & Answer

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Answer 5

Chapter 20, Problem 43P

Interpretation Introduction

(a)

Interpretation:

The values of kcatCO2/KMCO2 and kcatO2/KMO2 as s-¹M-1 should be determined.

Concept introduction:

If the concentration of enzyme is constant and the substrate concentration is gradually increased, the reaction velocity also increases up to a maximum and after that it will remain constant even though the substrate concentration increases further.

The Michael is constant, K_M is the substrate concentration at half of the maximum velocity. The smaller the K_M of an enzyme, the smaller the amount of substrate required to saturate the enzyme.

k_cat refers to the turn over number which is defined as the number of substrate molecules that is converted to product by each enzyme site per unit time.

Interpretation Introduction

(b)

Interpretation:

The reason for the occurrence of oxygenation reaction should be determined.

Concept introduction:

The ratio between turn over number and Michael is constant; kcatKM, is known as specificity constant. It is a measure of the efficiency of the enzyme on two competing substrates.

Answer 6

Chapter 20, Problem 43P

Interpretation Introduction

(a)

Interpretation:

The values of kcatCO2/KMCO2 and kcatO2/KMO2 as s-¹M-1 should be determined.

Concept introduction:

If the concentration of enzyme is constant and the substrate concentration is gradually increased, the reaction velocity also increases up to a maximum and after that it will remain constant even though the substrate concentration increases further.

The Michael is constant, K_M is the substrate concentration at half of the maximum velocity. The smaller the K_M of an enzyme, the smaller the amount of substrate required to saturate the enzyme.

k_cat refers to the turn over number which is defined as the number of substrate molecules that is converted to product by each enzyme site per unit time.

Answer 7

Chapter 20, Problem 43P

Interpretation Introduction

(a)

Interpretation:

The values of kcatCO2/KMCO2 and kcatO2/KMO2 as s-¹M-1 should be determined.

Concept introduction:

If the concentration of enzyme is constant and the substrate concentration is gradually increased, the reaction velocity also increases up to a maximum and after that it will remain constant even though the substrate concentration increases further.

The Michael is constant, K_M is the substrate concentration at half of the maximum velocity. The smaller the K_M of an enzyme, the smaller the amount of substrate required to saturate the enzyme.

k_cat refers to the turn over number which is defined as the number of substrate molecules that is converted to product by each enzyme site per unit time.

Answer 8

Interpretation Introduction

(b)

Interpretation:

The reason for the occurrence of oxygenation reaction should be determined.

Concept introduction:

The ratio between turn over number and Michael is constant; kcatKM, is known as specificity constant. It is a measure of the efficiency of the enzyme on two competing substrates.

Answer 9

Interpretation Introduction

(b)

Interpretation:

The reason for the occurrence of oxygenation reaction should be determined.

Concept introduction:

The ratio between turn over number and Michael is constant; kcatKM, is known as specificity constant. It is a measure of the efficiency of the enzyme on two competing substrates.

Answer 10

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Answer 11

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Answer 12

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Answer 13

Ch. 20 - Prob. 1P Ch. 20 - Prob. 2P Ch. 20 - Prob. 3P Ch. 20 - Prob. 4P Ch. 20 - Prob. 5P Ch. 20 - Prob. 6P Ch. 20 - Prob. 7P Ch. 20 - Prob. 8P Ch. 20 - Prob. 9P Ch. 20 - Prob. 10P

Solution Summary: The author explains how to calculate the ratios by substituting the values of k_catCO

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(b)

Interpretation:

The reason for the occurrence of oxygenation reaction should be determined.

Concept introduction:

The ratio between turn over number and Michael is constant; kcatKM, is known as specificity constant. It is a measure of the efficiency of the enzyme on two competing substrates.

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Chapter 20 Solutions

Solution Summary: The author explains how to calculate the ratios by substituting the values of k_catCO

Concept explainers

Videos

(b) Interpretation: The reason for the occurrence of oxygenation reaction should be determined. Concept introduction: The ratio between turn over number and Michael is constant; kcatKM, is known as specificity constant. It is a measure of the efficiency of the enzyme on two competing substrates.

Want to see the full answer?

Chapter 20 Solutions