Study Guide for Campbell Biology
11th Edition
ISBN: 9780134443775
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Jane B. Reece, Martha R. Taylor, Michael A. Pollock
Publisher: PEARSON
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Chapter 19, Problem 4TYK
Summary Introduction
Introduction: Retrovirus is a widespread infectious agent that has been identified in many eukaryotes including humans. Retroviruses are of great importance as they are mostly linked with diseases such as AIDS and cancers. Retroviruses are icosahedral viruses that have an envelope. They have single-stranded RNA as genetic material.
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When E. coli cells are mixed with recombinant vector DNA and subject to a stress such as heat shock, a small fraction of the cells will take up the plasmid DNA, a process known as :
A.
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Which of the following would be the first step in biosynthesis of a virus with a - (minus) strand of RNA?
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Plasmids are important in biotechnology because they are
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a. a vehicle for the insertion of foreign genes into bacteria
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Chapter 19 Solutions
Study Guide for Campbell Biology
Ch. 19 - In the following diagram of lytic and lysogenic...Ch. 19 - Summarize the flow of genetic information during...Ch. 19 - How does a virus spread throughout a plant? Are...Ch. 19 - Create a concept map that describes the lytic and...Ch. 19 - Prob. 2SYKCh. 19 - Prob. 1TYKCh. 19 - Prob. 2TYKCh. 19 - Prob. 3TYKCh. 19 - Prob. 4TYKCh. 19 - T2 and T4 are two types of bacteriophage that...
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- Which of the following cannot usually be inserted into a vector to form a recombinant DNA? A. PCR product of a eukaryotic coding sequence B. Lac operon operator region DNA sequence C. Reverse transcriptase gene D. E. coliarrow_forwardWhat is the function of reverse transcriptase in retroviruses? O A. It converts host cell RNA into viral DNA. B. It uses viral RNA as a template for making more viral RNA genomes. O. It translates viral RNA into proteins. D. It uses viral RNA as a template for DNA synthesis, that is, it makes complementary DNA.arrow_forwardDescribe how P1vir transduction can be used to introduce a gene mutation into E. coli. Use your own diagrams to aid your answer. Remember to refer to the figure in the text. You should describe / show both the molecular biology and the transduction procedure.arrow_forward
- If the HIV did not have a reverse transcriptase enzyme, then which of the following would apply? Select one: a. The virus can make use of the reverse transcriptase enzyme from the host cell to convert its RNA into DNA b. Viral particles will be produced without reverse transcriptase enzyme since the original HIV is an RNA virus and needs to assemble RNA ant coat proteins together c. The virus will manufacture its proteins from its RNA using translation and ribosomes from host cell d. Provirus will not be formed and assembly of viral particles will never occur e. The virus will not be able to recognise the host cell and therefore entry into the cell is blockedarrow_forwardWhich of the following is a concern of in-vivo gene therapy? A. Lytic vector expression B. Integration into target region C. Immune response to viral vector D. Production of chimeraarrow_forwardWhich of the following may be used as a vector in a gene-cloningexperiment?a. mRNA c. Virusb. Plasmid d. Both b and carrow_forward
- Bacteria exposed to viruses incorporate sections of the virus’s DNA into the CRISPR array sequences in their genome. This mechanism allows bacteria to fight off the viruses, like an immune response: the information in CRISPR spacers served as “coordinates” for recognizing and cutting up invading DNA sequences. Describe what might happen under the conditions described after a bacteriophage infects a bacterial cell and releases its DNA into the bacterial cell. Explain why: 1. The invading phage DNA is recognized by the Cas proteins but not inserted into the CRISPR array region of the bacterial genome: The bacteria will be unable to elicit an immune response and will succumb to the phase infection 2. The cas genes on the bacterial genome contains a missense mutation that increases its cleavage/cut activityThe bacteria will elicit an immune response that will successfully fight the phage infectionarrow_forwardhas been assembled by researchers and transplanted into a donor bacterial strain to study never before seen gene functions. Select one: a Transgenic genome b. Recombinant DNA sequence C. Knockdown gene d. Synthetic genome e. Recombinant plasmid xarrow_forwardWhat is the enzymatic function of restriction enzymes? Group of answer choices a. to cut nucleic acids at specific sites b. to join nucleotides during transcription c. to add new nucleotides to the growing strand of DNA d. to repair breaks in sugar - phosphate backbonesarrow_forward
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