Study Guide for Campbell Biology
11th Edition
ISBN: 9780134443775
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Jane B. Reece, Martha R. Taylor, Michael A. Pollock
Publisher: PEARSON
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Chapter 19, Problem 8TYK
Summary Introduction
Introduction: A virus is an infectious agent that cannot be seen through the naked eyes. Virology is the study of viruses and virologists are the biologists who study viruses. Viruses are obligate intracellular
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CHOICES:
a. Plasmid
b. Sticky end
c. DNA ligase
d. Transformation
e. Restriction enzyme
f. Genetic marker
g. Transduction
QUESTIONS:
Cuts the DNA into fragments
Circular DNA molecule of bacteria
Used to insert DNA of interest to vector
Area of DNA where bases are ready to be paired
Recombinant DNA technology with the help of a vector gene
When E. coli cells are mixed with recombinant vector DNA and subject to a stress such as heat shock, a small fraction of the cells will take up the plasmid DNA, a process known as :
A.
Ligation.
B.
Transformation.
C.
Transfection.
D.
Digestion.
has been assembled by researchers and transplanted into a donor bacterial strain to study never
before seen gene functions.
Select one:
a Transgenic genome
b. Recombinant DNA sequence
C. Knockdown gene
d. Synthetic genome
e. Recombinant plasmid x
Chapter 19 Solutions
Study Guide for Campbell Biology
Ch. 19 - In the following diagram of lytic and lysogenic...Ch. 19 - Summarize the flow of genetic information during...Ch. 19 - How does a virus spread throughout a plant? Are...Ch. 19 - Create a concept map that describes the lytic and...Ch. 19 - Prob. 2SYKCh. 19 - Prob. 1TYKCh. 19 - Prob. 2TYKCh. 19 - Prob. 3TYKCh. 19 - Prob. 4TYKCh. 19 - T2 and T4 are two types of bacteriophage that...
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- Describe how restriction enzymes like EcoR1 are used to create recombinant plasmids and what the process is for using these plasmids to replicate a piece of target DNA. Include information about how to create sticky ends, the makeup of the bacterial plasmid and how to tell if the gene was successfully inserted in the plasmid and if the plasmid has been transformed by the bacteria. You may use a drawing to enhance your description.arrow_forwardProteins that bind to a specific DNA sequence and help control the recruitment of RNA polymerase are called ... 1.) CRISPR/Cas9 2.) restriction enzymes 3.) transcription factors 4.) topoisomerasesarrow_forwardWhat are the sticky ends of the restriction fragments? Select one: a. The surfaces of sticky ends contain matching base pairs, allowing fragments to splice. b. The surfaces of sticky ends have glue like substance that allow fragments to splice. c. The surfaces of sticky ends contain the exact same nucleotides, allowing fragments to bond. d. The surfaces of sticky ends have velcro like structure, allowing fragments to bond.arrow_forward
- DEFINE THE FOLLOWING: 1) restriction enzyme 2) plasmid 3) recombinant DNAarrow_forwardWhich of the following is a primary participant in cloning an isolated gene? a. restriction endonuclease b. vector c. host organism d. all of thesearrow_forwardPlasmids are important in biotechnology because they are Group of answer choices a. a vehicle for the insertion of foreign genes into bacteria b. recognition sites on recombinant DNA strands c. surfaces for protein synthesis in eukaryotic recombinants d. viruses incorporated into the host DNAarrow_forward
- PCR is essential for Select one: A. allowing restriction enzymes to cut DNA. B. making many copies of a small amount of DNA. C. cutting DNA into many small pieces. D. creating recombinant plasmids.arrow_forwardWhat carries a gene from one organism into a bacteria cell? a. a plasmid b. an electrophoresis gel c. a restriction enzyme d. polymerase chain reactionarrow_forwardWhich of the following is not required for PCR? a. dNTPs b. bacterial plasmids c. carefully designed primers d. taq or other heat- resistant polymerases e. template DNAarrow_forward
- For which of the following would not require a nucleic acid probe?a. locating a gene on a chromosomeb. developing a Southern blotc. identifying a microorganism with FISHd. constructing a recombinant plasmidarrow_forwardExplain how electrophoresis separates DNA strands. a. How is a DNA fingerprinting test interpreted? b. Define plasmid and how plasmids can change a bacteria’s activity. c. How do we digest/cleave plasmids? Explain the role of a restriction enzyme. d. Define sticky end and blunt end and which one is useful in molecular biology.arrow_forwardThe function of a restriction enzyme is to a. prevent the movement of DNA outside the nucleus b. separate the DNA double helix c. cut the nucleotide sequence at a specific location in DNA d. proofread DNA for accidental damages and corrects these errorsarrow_forward
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