Genetics: From Genes to Genomes
6th Edition
ISBN: 9781259700903
Author: Leland Hartwell Dr., Michael L. Goldberg Professor Dr., Janice Fischer, Leroy Hood Dr.
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 19, Problem 28P
Some genes are required both zygotically and maternally. One experimental approach to studying such genes relies on the existence of ovoD , a dominant female sterile mutation of the ovo gene, which is located near the middle of the acrocentric Drosophila X chromosome. Females that are ovoD /ovo+ are sterile; ovoD>–containing germ-line cells cannot produce eggs.
a. | Mutations in gene X are recessive lethals, so homozygotes for these mutations do not develop into adults. Explain how researchers could use the ovoD mutation in a mitotic recombination experiment to determine (i) whether or not females might supply the RNA or protein product of gene X to the eggs they make in their ovaries, and (ii) whether this maternally supplied product is needed for proper development of their progeny. Where in the genome would gene X need to be located for this approach to work? |
b. | The ovoD mutant gene has been cloned, so genomic DNA for this mutant gene is available. How could you use this cloned DNA to determine whether any embryonic lethal mutation located anywhere in the genome was an allele of a maternal effect gene? |
c. | Regardless of its chromosomal location, how could you distinguish in such an experiment whether the gene in question was a maternal effect gene, as opposed to a gene whose product is needed for oogenesis in the female? |
d. | How can fly strains containing ovoD mutations be maintained if females carrying these mutations are sterile? |
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Chapter 19 Solutions
Genetics: From Genes to Genomes
Ch. 19 - Match each of the terms in the left column to the...Ch. 19 - a. If you were interested in the role of a...Ch. 19 - Early C. elegans embryos display mosaic...Ch. 19 - Hypomorphic mutations in the wingless gene of...Ch. 19 - Prob. 5PCh. 19 - In 1932, H. J. Muller suggested a genetic test to...Ch. 19 - a. Explain how you could use worms transformed...Ch. 19 - Suppose you want to determine whether a particular...Ch. 19 - Sevenless is an unusual receptor protein in that...Ch. 19 - Suppose that you generated flies containing a...
Ch. 19 - Drosophila researchers have collected many strains...Ch. 19 - As an alternative to random mutagenesis,...Ch. 19 - A C. elegans nematode gene called par-1 helps to...Ch. 19 - The molecular identity of the fruit fly rugose...Ch. 19 - To determine the focus of action of boss,...Ch. 19 - Suppose a particular gene is required for early...Ch. 19 - Researchers have exploited Minute mutations in...Ch. 19 - Some ts alleles are temperature sensitive during...Ch. 19 - The following figure shows the temperature-shift...Ch. 19 - A temperature-sensitive allele of the gene...Ch. 19 - Hypomorphic alleles of a pleiotropic gene...Ch. 19 - In addition to the maternal effect genes that...Ch. 19 - The yan gene encodes a transcription factor that...Ch. 19 - Recall from Chapter 17 that in Drosophila, sex...Ch. 19 - a. Explain the difference between maternal...Ch. 19 - In the 1920s, Arthur Boycott, working with the...Ch. 19 - The Drosophila mutant screen shown on the...Ch. 19 - Some genes are required both zygotically and...Ch. 19 - How would a human with a mutation in a maternal...Ch. 19 - One important demonstration that Bicoid is an...Ch. 19 - The hunchback gene contains a 5 transcriptional...Ch. 19 - In flies developing from eggs laid by a...Ch. 19 - Wild-type embryos and mutant embryos lacking the...Ch. 19 - The Drosophila even-skipped eve gene has four...Ch. 19 - In Drosophila with loss-of-function mutations...Ch. 19 - It is crucial to the development of Drosophila...Ch. 19 - In the plant Arabidopsis thaliana, every flower is...
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- I. Male Drosophila from a true-breeding wild-typestock were irradiated with X-rays and then mated withfemales from a true-breeding stock carrying the following recessive mutations on the X chromosome:yellow body (y), crossveinless wings (cv), cut wings(ct), singed bristles (sn), and miniature wings (m).These markers are known to map in the order:y - cv - ct - sn - mMost of the female progeny of this cross were phenotypically wild type, but one female exhibited ct and snphenotypes. When this exceptional ct sn female wasmated with a male from the true-breeding wild-typestock, twice as many females as males appearedamong the progeny.a. What is the nature of the X-ray-induced mutationpresent in the exceptional female?b. Draw the X chromosomes present in the exceptional ct sn female as they would appear duringpairing in meiosis.c. What phenotypic classes would you expect to seeamong the progeny produced by mating the exceptional ct sn female with a normal male from a truebreeding wild-type…arrow_forwardIt is assumed that in Drosophila the following genotypes produce phenotypes. َA- B- = Red color A- bb = Plum color aa B- = Magenta color aa bb = White color The third latent genotype, cc, kills homozygous Plums, but has no effect on other genotypes. Also, genotype C- does not produce a large phenotype. If first-generation Drosophilas are heterozygous for all of these genes and interbreed, what phenotypic ratios are expected in society?arrow_forwardCertain genes determine sex, fertility, and viability in Drosophila, and mutations in these genes affect the two sexes differently. Classify the descriptions below based on their occurrence in XY or XX flies. Some descriptions may be used in both categories. The expressed form of Dsx activates maleness genes and represses femaleness genes. XY XX Null mutations in the dsx gene result in an intersex phenotype. Loss-of-function mutations in the tra gene have no effect on phenotype. Sxl is expressed in early embryogenesis from the establishment promoter. Flies that produce Fru-M in brain cells from a transgene perform a mating dance and specifically court females. Loss-of-function mutations in the fru gene result in flies who court both males and females. Sxl regulates splicing of its own RNA to produce a functional protein. The expressed form of Dsx activates femaleness genes and represses maleness genes. Loss-of-function mutations in the Sxl gene have no effect on phenotype. Resetarrow_forward
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