Genetics: From Genes to Genomes
6th Edition
ISBN: 9781259700903
Author: Leland Hartwell Dr., Michael L. Goldberg Professor Dr., Janice Fischer, Leroy Hood Dr.
Publisher: McGraw-Hill Education
expand_more
expand_more
format_list_bulleted
Videos
Textbook Question
Chapter 19, Problem 11P
Drosophila researchers have collected many strains that carry a single recombinant P element containing a wild-type white gene (a P[w+] transgene) inserted into a known genomic location. These strains can be used to map the location of any mutant gene in the fly genome.
Investigators performed a testcross to map a recessive mutation rough (ro), which causes rough eyes, relative to a P[w+] element on chromosome 3. Females heterozygous for the P[w+] on one chromosome 3 and a ro− mutation on the other, homologous chromosome 3 were crossed to ro−/ro− males, and the progeny in the following list were obtained. In both the parents and the progeny, the endogenous white gene is nonfunctional—the flies have red eyes only if they contain the P[w+] transgene.
| a. | Are ro and the P[w+] linked? If so, how many map units separate them? |
| b. | The data in part (a) do not indicate on which side of the P[w+] (toward the centromere or telomere) the ro gene is located. How could the experiment be modified to reveal that information? |
| c. | Suppose you map the ro mutation to a genomic region between two different P[w+] elements that are 5000 bp apart. Describe some experimental approaches that would allow you to identify the ro gene at the molecular level. |
| d. | How could you use the DNA sequence of the ro gene to determine the function of the protein it encodes? |
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
QUESTION 11
The location of five deletions have been mapped to a Drosophila chromosome, as shown in the following deletion map.
Recessive mutations a, b, c, d and e are known to be located in the same region as the deletions but the order of the
mutations on the chromosome is not known.
Drosophila Chromosome
Deletion 1
Deletion 2
Deletion 3
Deletion 4
Deletion 5
When flies homozygous for the recessive mutation are crossed with flies heterozygous for the deletions, the following
results are obtained in which "m" represents recovery of flies with a mutant phenotype and "+" represents recovery of
only flies with a wildtype phenotype. Use the data from the table below to determine the order of the genes (a-e) on the
chromosome.
Mutants
Deletions
a
b
C
d
e
1
+
+
+
+
m
2
+
m
+
m
3
m
m
m
m
4
+
+
m
+
+
5
m
+
m
m
+
What is the gene order for these five genes? Please do not separate the letters representing the mutations with
spaces or punctuation (e.g. LMNOP)
The mutations called bobbed in Drosophila result from variable reductions (deletions) in the number of amplified genes coding for rRNA. Researchers trying to maintain bobbed stocks have often documented their tendency to revert to wild type in successive generations. Propose a mechanism based on meiotic recombination which could account for this reversion phenomenon. Why would wild-type flies become more prevalent in Drosophila cultures?
A yeast geneticist irradiates haploid cells of a strain that is an adenine-requiring auxotrophic mutant, caused by mutation of the gene ade1. Millions of the irradiated cells are plated on minimal medium, and a small number of cells divide and produce prototrophic colonies. These colonies are crossed individually with a wildtype strain. Two types of results are obtained:(1) prototroph × wild type : progeny all prototrophic(2) prototroph × wild type : progeny 75% prototrophic, 25% adenine-requiring auxotrophsa. Explain the difference between these two types of results.b. Write the genotypes of the prototrophs in each case.c. What progeny phenotypes and ratios do you predict from crossing a prototroph of type 2 by the original ade1auxotroph?
Chapter 19 Solutions
Genetics: From Genes to Genomes
Ch. 19 - Match each of the terms in the left column to the...Ch. 19 - a. If you were interested in the role of a...Ch. 19 - Early C. elegans embryos display mosaic...Ch. 19 - Hypomorphic mutations in the wingless gene of...Ch. 19 - Prob. 5PCh. 19 - In 1932, H. J. Muller suggested a genetic test to...Ch. 19 - a. Explain how you could use worms transformed...Ch. 19 - Suppose you want to determine whether a particular...Ch. 19 - Sevenless is an unusual receptor protein in that...Ch. 19 - Suppose that you generated flies containing a...
Ch. 19 - Drosophila researchers have collected many strains...Ch. 19 - As an alternative to random mutagenesis,...Ch. 19 - A C. elegans nematode gene called par-1 helps to...Ch. 19 - The molecular identity of the fruit fly rugose...Ch. 19 - To determine the focus of action of boss,...Ch. 19 - Suppose a particular gene is required for early...Ch. 19 - Researchers have exploited Minute mutations in...Ch. 19 - Some ts alleles are temperature sensitive during...Ch. 19 - The following figure shows the temperature-shift...Ch. 19 - A temperature-sensitive allele of the gene...Ch. 19 - Hypomorphic alleles of a pleiotropic gene...Ch. 19 - In addition to the maternal effect genes that...Ch. 19 - The yan gene encodes a transcription factor that...Ch. 19 - Recall from Chapter 17 that in Drosophila, sex...Ch. 19 - a. Explain the difference between maternal...Ch. 19 - In the 1920s, Arthur Boycott, working with the...Ch. 19 - The Drosophila mutant screen shown on the...Ch. 19 - Some genes are required both zygotically and...Ch. 19 - How would a human with a mutation in a maternal...Ch. 19 - One important demonstration that Bicoid is an...Ch. 19 - The hunchback gene contains a 5 transcriptional...Ch. 19 - In flies developing from eggs laid by a...Ch. 19 - Wild-type embryos and mutant embryos lacking the...Ch. 19 - The Drosophila even-skipped eve gene has four...Ch. 19 - In Drosophila with loss-of-function mutations...Ch. 19 - It is crucial to the development of Drosophila...Ch. 19 - In the plant Arabidopsis thaliana, every flower is...
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Consider a maize plant: Genotype C/cm ; Ac/Ac+ where cm is an unstable colorless allele caused by Ds insertion. What phenotypic ratios would be produced and in what proportions when this plant is crossed with a mutant c/c Ac+/Ac+? Assume that the Ac and c loci are unlinked, that the chromosome-breakage frequency is negligible, and the C allele encodes pigment production.arrow_forwardYou are studying four linked genes located on chromosome 2 in the fruit fly Drosophila melanogaster: adp (which contributes to obesity), b (which contributes to body color), pr (which contributes to metabolism), and vg (which contributes to wing formation). A series of crosses between pair-wise combinations of these mutations yielded the following recombination frequencies between the indicated loci: pr and adp 9% adp and b 6% pr and b 3% vg and b 10% vg and pr 7% What is the genetic distance in map units (cM) between the adp and vg loci? 16 9 6 4arrow_forwardDrosophila females heterozygous for three recessive mutations, a, b, and c , were crossed to males homozygous for all three mutations.The cross yielded the following results: in the image Q. Construct a linkage map showing the correct order of these genes and estimate the distances between them.arrow_forward
- The normal sequence of markers on a certain Drosophila chromosome is ABCDE*FGHIJK, where the asterisk represents the centromere. Some flies were isolated with a chromosome aberration that has the following structure: ABCDE*FIJK . This represents a O a) deletion of GH segment O b) inversion of GH segment O c) deletion O d) deletion of centromerearrow_forwardIn a mutant screen in Drosophila, you identified a gene related to memory, as evidenced by the inability of recessive homozygotes to learn to associate a particular scent with the availability of food. Given another line of flies with an autosomal mutation that produces orange eyes, design a series of crosses to determine the map distance between these two loci. You do not need to calculate recombination frequency.arrow_forwardIn an electrophoretic gel across which is applied a powerful electrical alternating pulsed field, the DNA of the haploid fungus Neurospora crassa (n = 7) moves slowly but eventually forms seven bands, which represent DNA fractions that are of different sizes and hence have moved at different speeds. These bands are presumed to be the seven chromosomes. How would you show which band corresponds to which chromosome?arrow_forward
- The genome of Drosophila has been studied extensively using the technique of gene mapping. In Drosophila, pink eyes (P), curled wings (C), hairy body (H), and ebony body (E) are determined by alleles of genes located on the same chromosome. Gene Combination E/H C/H E/P H/P Frequency of Recombination 44% 23% 23% 21% Draw a gene map showing the correct order these genes appear on the chromosome. How frequently are the genes for pink eyes and curled wings expected to separate during crossing over?arrow_forwardIn Drosophila, Lyra (Ly) and stubble (Sb) are dominant mutations located at locus 40 and 58, on chromosome 3. A recessive mutation with bright eyes was discovered and shown also to be on chromosome 3. A map was obtained by crossing a female who was heterozygous for all three mutations to a male homozygous for the bright red mutation (temporarily will be called br). The following data were obtained. Ly Sb br 404 + + br 2 Ly + br 75 + Sb + 59 Ly + + 18 + Sb br 16 Ly Sb + 4 + + + 422 Diagram the cross and determine the location of the bright red mutation on chromosome 3arrow_forwardIf you mated an F' bacterial cell with lacl+ lacP+ lacO+ lacZ+ lacY+ on the bacterial chromosome and lacl+ lacP+ lacOc lacZ+ lacY- on a plasmid with an F- lacl+ lacP+ lacO+ lacZ+ lacY+ and lactose was absent and glucose was absent, what expression levels do you predict in the RECIPENT cell after conjugation (assume no recombination)? Betagalactosidase expression will be HIGH Betagalactosidase expression will be BASAL Betagalactosidase expression will be LOWER THAN BASAL There will be NO Betagalactosidase expressionarrow_forward
- A 5-year-old boy with mental retardation is grossly obese and has facial features of Prader-Willi syndrome (PWS). Karyotyping and fluorescent in situ hybridization studies do not show deletion in the usual site on chromosome 15. Which of the following findings is most likely to confirm PWS in this child? ✓ A Deletion in the short arm of chromosome 15 BY Duplication within chromosome 15 Large trinucleotide repeat expansion in the PWS area of chromosome 15 D) Maternal origin of both chromosomes 15 -Translocation in the short arm of chromosome 15arrow_forwardIn an in situ hybridization experiment, a certain clonebound to only the X chromosome in a boy with no diseasesymptoms. However, in a boy with Duchenne musculardystrophy (X-linked recessive disease), it bound to theX chromosome and to an autosome. Explain. Could thisclone be useful in isolating the gene for Duchenne muscular dystrophy?arrow_forwardOne of the X chromosomes in a particular Drosophila female had a normal order of genes but carried recessive alleles of the genes for yellow body color (y), vermilion eye color (v), and forked bristles (f), as well as the dominant X-linked Bar eye mutation (B). Her other X chromosome carried the wild-type alleles of all four genes, but the region including y, v, and f (but not B) was inverted with respect to the normal order of genes. This female was crossed to a wild-type male as diagrammed here. 1. Draw the meiotic prophase I chromosomes with maximal pairing from the inversion heterozygous female. 2. (continued) When a crossover occurs between v and f, please draw the resulting chromosomes in four gametes.arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSON
Biology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStax
Anatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:PEARSON
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
Anatomy & Physiology
Biology
ISBN:9781259398629
Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:9780815344322
Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:9781260159363
Author:Martin, Terry R., Prentice-craver, Cynthia
Publisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:9781260231700
Author:Sylvia S. Mader, Michael Windelspecht
Publisher:McGraw Hill Education
Embryology | Fertilization, Cleavage, Blastulation; Author: Ninja Nerd;https://www.youtube.com/watch?v=8-KF0rnhKTU;License: Standard YouTube License, CC-BY