Interpretation:
The graph which represents the activity curve of the reaction in which EDTA is added to a reaction mediated by EcoRI has to be determined.
Concept Introduction:
Enzyme inhibitors: The substance which slows or stops the action of an enzyme is called enzyme inhibitors.
It can be used as drugs and an example is AZT which is used to treat HIV.
The competition of an enzyme can be reversible or irreversible. In reversible inhibition, the inhibitor can leave and in irreversible inhibition, the inhibitor remains permanently bound.
Reversible Competitive inhibition: It is a type of inhibition occurs when the inhibitor resembles very much to the substrate and thus prevents the substrate binding.
Irreversible competitive inhibition: It is a type of inhibition in which an inhibitor forms covalent bonds to the active site and thereby permanently blocking it.
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Fundamentals of General, Organic, and Biological Chemistry (8th Edition)
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