Essentials of Genetics (9th Edition) - Standalone book
9th Edition
ISBN: 9780134047799
Author: William S. Klug, Michael R. Cummings, Charlotte A. Spencer, Michael A. Palladino
Publisher: PEARSON
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Chapter 19, Problem 10PDQ
What limits the use of differences in restriction enzyme sites as a way of detecting point mutations in human genes?
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RFLP's result from mutations in:
1)
genes which specify restriction enzymes
2) restriction enzyme recognition sites
3)
genes which specify plasmids
4) monomorphic genes
What sequence do the restriction enzymes used in the lab recognize? How do they cut? And how would these different cuts effect cloning? (i.e. compare and contrast how overhang and blunt cuts will differ with cloning.)
What organisms were the restriction enzymes used in this lab derived from?
Why are restriction enzymes important for the organism that makes them?
What is the purpose of multiple cloning sites on pUC19? What size were each of your plasmid fragments?
Describe the nature of recognition sites for restriction enzymes and the nature of the ends of the DNA that are left. Why do we need to run a gel electrophoresis after enzyme digestion
Chapter 19 Solutions
Essentials of Genetics (9th Edition) - Standalone book
Ch. 19 - CASE STUDY| Cancer-killing bacteria Ralph, a...Ch. 19 - CASE STUDY| Cancer-killing bacteria Ralph, a...Ch. 19 - CASE STUDY |Cancer-killing bacteria Ralph, a...Ch. 19 - HOW DO WE KNOW? In this chapter, we focused on a...Ch. 19 - Prob. 2PDQCh. 19 - Why are most recombinant human proteins produced...Ch. 19 - Prob. 4PDQCh. 19 - One of the major causes of sickness, death, and...Ch. 19 - Prob. 6PDQCh. 19 - Sequencing the human genome and the development of...
Ch. 19 - Prob. 8PDQCh. 19 - As genetic testing becomes widespread, medical...Ch. 19 - What limits the use of differences in restriction...Ch. 19 - Might it make sense someday to sequence every...Ch. 19 - What is the main purpose of genome-wide...Ch. 19 - Prob. 13PDQCh. 19 - Prob. 14PDQCh. 19 -
15. In 2013 the actress Angelina Jolie elected to...Ch. 19 - Prob. 16PDQCh. 19 - Should the FDA regulate direct-to-consumer genetic...Ch. 19 - Prob. 18PDQCh. 19 - Prob. 19PDQCh. 19 - Private companies are now offering personal DNA...Ch. 19 - Yeager, M., et al.(Nature Genetics 39: 645–649,...Ch. 19 - In March 2010 Judge R. Sweet ruled to invalidate...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Name the mapping technique used to determine the position of restriction sites in a DNA molecule.arrow_forwardHow can we use software to identify restriction-enzyme cutting sites in sequenced DNA ?arrow_forwardHow does the use of restriction endonucleases of different specificities aid in the sequencing of DNA?arrow_forward
- Is it necessary to cut the isolated genomic DNA of an organism with restriction enzymes for analyzing a particular gene sequence? Discuss.arrow_forwardHow do tou figure out which type of restriction enzymes to use in an experiment and what would happen if they were damaged and didn't work?arrow_forwardWhat are the three types of DNA ends that can be generated after cutting DNA with restriction enzymes? What reaction is catalyzed by DNA ligase?arrow_forward
- Describe two features that are characteristic of the action of restriction endonucleases. How do these features make restriction endonucleases useful to genetic engineers?arrow_forwarda) What are vectors? Describe extensively the roles vectors play in genetic engineering? Write short notees on the following: Recombinant DNA, Cloning b) What are restriction enzymes? Describe extensively the roles restriction enzymes play in genetic engineering? Write short notees on the following: Selectable markers, Cloningarrow_forwardWhat are selectable markers and why are they important to cloning vectors?arrow_forward
- What are the key differences between DNA microarrays and protein microarrays, and how they are used in research?arrow_forwardb) Describe how DNA is digested by different restriction enzymes c) Describe how gel electrophoresis is used to estimate the size of DNA fragments.arrow_forwardWhat is the function of a restriction enzyme?arrow_forward
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