Essentials of Genetics (9th Edition) - Standalone book
9th Edition
ISBN: 9780134047799
Author: William S. Klug, Michael R. Cummings, Charlotte A. Spencer, Michael A. Palladino
Publisher: PEARSON
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Chapter 19, Problem 3PDQ
Why are most recombinant human proteins produced in animal or plant hosts instead of bacterial host cells?
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What is a recombinant vector? How is a recombinant vector constructed? Explain how X-Gal is used in a method of identifying recombinant vectors that contain segments of chromosomal DNA.
What is the difference between a recombinant protein and a native protein? Why is it that some amount of expressions of recombinant protein are toxic compared to the same amounts of native proteins?
Consider the following experiment. First, large populations of two mutant strains of Escherichia coli are mixed, each requiring a different, single amino acid. After plating them onto a minimal medium, 45 colonies grew. Which of the following may explain this result?
A) The colonies may be due to back mutation (reversion).
B) The colonies may be due to recombination.
C) Either A or B is possible.
D) Neither A nor B is possible.
Chapter 19 Solutions
Essentials of Genetics (9th Edition) - Standalone book
Ch. 19 - CASE STUDY| Cancer-killing bacteria Ralph, a...Ch. 19 - CASE STUDY| Cancer-killing bacteria Ralph, a...Ch. 19 - CASE STUDY |Cancer-killing bacteria Ralph, a...Ch. 19 - HOW DO WE KNOW? In this chapter, we focused on a...Ch. 19 - Prob. 2PDQCh. 19 - Why are most recombinant human proteins produced...Ch. 19 - Prob. 4PDQCh. 19 - One of the major causes of sickness, death, and...Ch. 19 - Prob. 6PDQCh. 19 - Sequencing the human genome and the development of...
Ch. 19 - Prob. 8PDQCh. 19 - As genetic testing becomes widespread, medical...Ch. 19 - What limits the use of differences in restriction...Ch. 19 - Might it make sense someday to sequence every...Ch. 19 - What is the main purpose of genome-wide...Ch. 19 - Prob. 13PDQCh. 19 - Prob. 14PDQCh. 19 -
15. In 2013 the actress Angelina Jolie elected to...Ch. 19 - Prob. 16PDQCh. 19 - Should the FDA regulate direct-to-consumer genetic...Ch. 19 - Prob. 18PDQCh. 19 - Prob. 19PDQCh. 19 - Private companies are now offering personal DNA...Ch. 19 - Yeager, M., et al.(Nature Genetics 39: 645–649,...Ch. 19 - In March 2010 Judge R. Sweet ruled to invalidate...
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- Are they true or false? a)If someone reads an absorbance of 0,25 at 260 nm, the concentration of the dsDNA is 25 ug/ml. b)If someone reads an absorbance of 0,25 at 260 nm, the concentration of the dsDNA is 25 ug/ml c)The copy number refers to the number of molecules of an individual plasmid that are normally found in a single bacterial cell. d)The amount of mRNA level can be determined via competitor RT-PCR in which two different sized products can be obtained, one of which is a known standard.arrow_forwardWhich of the following pieces of information about our recombinant DHFR protein do you predict to obtain from Western Blot analysis? A) Protein size B) Protein sequence C) Protein functionarrow_forwardWe have two specific strains of E. coli that have shown horizontal gene transfer (HGT) when mixed. To experimentally determine the method of HGT that is happening, the following conditions are set up in different tubes of culture media: A) Donor and recipient strain mixed together (control - no treatment). B) Donor and recipient strains mixed together, DNase added (can digest DNA in solution, not within cells).C) Special tube containing a membrane filter (with pores that allow DNA and viruses to pass through, but not bacterial cells) that separates two compartments. Donor strain is added on one side, the recipient strain on the other (they are separated by the filter).D) Donor and recipient strains mixed together, with chemical that inactivates viruses (chemical affects bacteriophages in solution so they are unable to attach to cells). The results: Tubes A, B, and D: HGT was observed. Tube C: HGT was NOT observed. Based on this, which type of HGT was occurring? Conjugation,…arrow_forward
- Consider three genes in E. coli: thr+, ara+, and leu+ (which give the cell the ability to synthesize threonine, arabinose, and leucine, respectively). All three of these genes are close together on the E. coli chromosome. Phages are grown in a thr+ ara+ leu+ strain of bacteria (the donor strain). The phage lysate is collected and used to infect a strain of bacteria that is thr− ara− leu −. The recipient bacteria are then tested on selective medium lacking leucine. Bacteria that grow and form colonies on this medium (leu+ transductants) are then replica-plated on medium lacking threonine and on medium lacking arabinose to see which are thr+ and which are ara+. Another group of the recipient bacteria are tested on medium lackingthreonine. Bacteria that grow and form colonies on this medium (thr+ transductants) are then replica-plated on medium lacking leucine and onto medium lacking arabinose to see which are ara+ and which are leu+. Results from these experiments are as follows:…arrow_forwardWhy are the recombinantDNA technology and thenucleus transplantationtechnology still dangerous?arrow_forwardWhy is lambda DNA used as a marker?arrow_forward
- Plasmids are the only vectors currently available for use in recombinant procedures.True or false?arrow_forwardWhy are plasmids used as vector for DNA Recombination? What other vectors can be used?arrow_forwardHow can one prevent recombinant DNA from undergoing recombination in a bacterial host cell?arrow_forward
- High Frequency Recombination results in which of the following? O 1) Plasmid DNA incorporated into the cell 2) Genomic DNA incorporated into the cell 3) Viral DNA incorporated into the cell 4) Plasmid and genomic DNA from the donor cell is incorporated into the recipient cell's genome 5) The movement of genes within the cell through homologous recombinationarrow_forwardDNA from a strain of bacteria with genotype a+ b+ c+ d+ e+ was isolated and used to transform a strain of bacteria that was a− b− c− d− e−. The transformants were tested for the presence of donated genes. The following genes were cotransformed: a+ and d+ b+ and e+ c+ and d+ c+ and e+ What is the order of genes a, b, c, d, and e on the bacterial chromosome?arrow_forwardE. coli cells are simultaneously infected with two strains of phage λ. One strain has a mutant host range, is temperature sensitive, and produces clear plaques (genotype h st c); another strain carries the wildtype alleles (genotype h+ st+ c+). Progeny phages are collected from the lysed cells and are plated on bacteria. The following numbers of different progeny phages are obtained: Progeny phage genotype Number of plaques h+ c+ st+ 321 h c st 338 h+ c st 26 h c+ st+ 30 h+ c st+ 106 h c+ st 110 h+ c+ st 5 h c st+ 6 a. Determine the order of the three genes on the phage chromosome. b. Determine the map distances between the genes. c. Determine the coefficient of coincidence and the interferencearrow_forward
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genetic recombination strategies of bacteria CONJUGATION, TRANSDUCTION AND TRANSFORMATION; Author: Scientist Cindy;https://www.youtube.com/watch?v=_Va8FZJEl9A;License: Standard youtube license