BIOLOGY
12th Edition
ISBN: 9781260169614
Author: Raven
Publisher: RENT MCG
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Chapter 17, Problem 5U
In terms of studying gene function, what is the main benefit that genome editing has over RNAi?
a. Genome editing can eliminate gene function; RNAi simply reduces the levels of gene products.
b. Genome editing does not require that recombinant DNA be produced, whereas RNAi always does.
c. Genome editing always works, but RNAi rarely works.
d. Genome editing is done in bacteria, which are easier to manipulate than the eukaryotic cells in which RNAi is done.
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Chapter 17 Solutions
BIOLOGY
Ch. 17.1 - Prob. 1LOCh. 17.1 - Prob. 2LOCh. 17.1 - Describe the construction and uses of recombinant...Ch. 17.2 - Relate the process of DNA replication to PCR.Ch. 17.2 - Compare and contrast PCR, RT-PCR, and quantitative...Ch. 17.3 - Prob. 1LOCh. 17.3 - Prob. 2LOCh. 17.3 - Describe the pros and cons of RNA interference and...Ch. 17.4 - Explain how the universal nature of the genetic...Ch. 17.4 - Compare and contrast knockout, knockin, and...
Ch. 17.4 - Prob. 3LOCh. 17.5 - Describe the benefits of biofuel production from...Ch. 17.5 - Prob. 2LOCh. 17.5 - Prob. 3LOCh. 17.6 - Prob. 1LOCh. 17.6 - Compare and contrast FISH and gene chip...Ch. 17.6 - Describe how immunoassays can be used to diagnose...Ch. 17.7 - Describe the benefits of creating transgenic...Ch. 17.7 - Prob. 2LOCh. 17.7 - Evaluate issues on each side of the transgenic...Ch. 17 - Prob. 1DACh. 17 - Prob. 2DACh. 17 - Prob. 1IQCh. 17 - Prob. 2IQCh. 17 - You study a gene known to be important in the...Ch. 17 - What is the basis of separation of different DNA...Ch. 17 - Prob. 3UCh. 17 - FISH analysis of a breast tumor biopsy for HER2...Ch. 17 - In terms of studying gene function, what is the...Ch. 17 - The Ti plasmid of Agrobacterium usually induces...Ch. 17 - Prob. 1ACh. 17 - Which of the following statements is accurate for...Ch. 17 - Prob. 3ACh. 17 - Many human proteins, such as hemoglobin, are only...Ch. 17 - Amyloid beta is a proteolytic product of a protein...
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- How can you design your RT-PCR experiment to control for gDNA contamination? A. Use forward and reverse primers that bind to the same exon. B. Run a control lane where only RT was performed and not PCR. C. Run a control lane where MRNA has been amplified using PCR. D. Use forward and reverse primers that span the junction of 2 separate exons.arrow_forwardA student wants to observe how the environment of bacteria affects gene expression. How should the experiment be designed? A. exposing the same species of bacteria to the same carbohydrates in each sample B. exposing the same species of bacteria to different carbohydrates in each sample C. exposing different species of bacteria to the same carbohydrates in each sample D. exposing different species of bacteria to different carbohydrates in each samplearrow_forwardCRISPR is a tool used by bacteria to fight virus attacks. In your own words, describe how CRISPR works in bacterial systems, and how this idea can be used for genome editing.arrow_forward
- What is a proteome? a. The collection of all genes encoding proteins b. The collection of all proteins encoded by the genome c. The collection of all proteins present in a cell d. The amino acid sequence of a proteinarrow_forwardPart A: During cloning, the DNA is cut with a restriction enzyme giving it what? A. more introns B. sticky ends C. a polyA tail D. a binding site for a transcription factor Part B: If the scientist wanted to have a large number of copies of the gene for use in further study, which technique would the scientist use? A. PCR B. epigenetics C. hybridization D. gel electrophoresis Part C: If this gene were found to be expressed at different levels in different cells of the same organism, what would be responsible? A. transcription factor B. rRNA C. ribosome D. RNA polymerase Part D: If a disease were identified as being caused by defects in the cytochrome c gene, then the copy isolated could be used for what? A. PCR B. gene therapy C. stem cell work D. DNA fingerprintingarrow_forwardIn next-generation sequencing, which of these advances allows for massively parallel sequencing? a. Pieces of DNA are fixed to a surface, so we can tell which new nucleotides were added to each piece. b. DNA sequences are read in real-time as nucleotides are added to each piece. c. Each segment of the genome can be pieced back together through shotgun alignment d. Single molecules of DNA can be read without the need for amplification.arrow_forward
- How is a fusion protein formed? a. Use CRISPR/Cas9 to remove a portion of the gene of interest, thereby creating a shorter protein b. Use site-directed mutagenesis to replace one sequence with another c. Use the yeast two hybrid technique to form a new hybrid protein d. Use recombinant DNA to ligate a cDNA in frame with another protein coding sequencearrow_forwardGenetic engineering is the direct modification of an organisms’ DNA using biotechnology. What are the different techniques in genetic engineering? a. Inoculate, Gel electrophoresis, Budding and Cloning b. Grafting, Gene splicing, Budding and Inbreeding c. Cloning, Gene splicing, Gel electrophoresis and Hybridization d. Gene splicing, Grafting, Hybridization and Cloningarrow_forwardWhat is the difference between orthologs and paralogs? a. Orthologs are homologous sequences; paralogs are analogous sequences. b. Orthologs are more similar than paralogs. c. Orthologs are in the same species; paralogs are in different species. d. Orthologs are in different species; paralogs are in the same species.arrow_forward
- If you were to add all of the components necessary for transcription to a test tube, which nucleic acids would you find in the test tube after transcription termination? a. Double-stranded DNA b. Single-stranded DNA c. Double-stranded RNA d. Single-stranded RNA e. Double-stranded RNA/DNA hybrid f. A and Darrow_forwardWhich type of mutation would expect would have no effect on a protein coding gene in eukaryotes? a.a single base substitution that creates a splice site mutation b.a single base substitution that creates a synonymous mutation c.a single base deletion that creates a frameshift near the 3' end of the open reading frame d.a single base substitution that creates a non-conservative missense mutation e.a single base substitution that creates a conservative missense mutationarrow_forwardThe CRISPR system may be used to manipulate the genome in human cells, because Cas9 can Select one: O a. methylate specific RNA sequence of target gene. O b. cleave double-stranded RNA sequence of target gene. O c. methylate double-stranded DNA sequence of target gene. O d. cleave double-stranded DNA sequence of target gene.arrow_forward
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