BIOLOGY
12th Edition
ISBN: 9781260169614
Author: Raven
Publisher: RENT MCG
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Chapter 17, Problem 3A
Summary Introduction
Introduction:
Transgenic organisms contain a gene called as transgene from a different species, which has incorporated into their genetic composition through genetic engineering. The technique of genetic engineering also allows removal and change of gene as well. For example, a human gene can be placed in bacterial genome and the bacteria will encode the proteins of human.
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What is the role of Agrobacterium tumefaciens in the production of transgenic plants?a. Genes from A. tumefaciens are inserted into plant DNA to give the plant different traits.b. Transgenic plants have been given resistance to the pest A. tumefaciens.c. A. tumefaciens is used as a vector to move genes into plant cells.d. Plant genes are incorporated into the genome of Agrobacterium tumefaciens
The reason why Beadle and Tatum observed four different categories of mutants that could not grow on media without methionine is because
a. the enzyme involved in methionine biosynthesis is composed of four different subunits.
b. the enzyme involved in methionine biosynthesis is present in four copies in the Neurospora genome.
c. four different enzymes are involved in a pathway for methionine biosynthesis.
d. a lack of methionine biosynthesis can inhibit Neurospora growth in four different ways.
It has been suggested that it would make the study of human diseases easier if cloned transgenic animals
were produced that carried faulty versions of human genes (e.g., the gene that causes cystic fibrosis).
a. Why would such animals be useful in medical research? :
b. What ethical questions are raised by the creation of such transgenic animals?
Chapter 17 Solutions
BIOLOGY
Ch. 17.1 - Prob. 1LOCh. 17.1 - Prob. 2LOCh. 17.1 - Describe the construction and uses of recombinant...Ch. 17.2 - Relate the process of DNA replication to PCR.Ch. 17.2 - Compare and contrast PCR, RT-PCR, and quantitative...Ch. 17.3 - Prob. 1LOCh. 17.3 - Prob. 2LOCh. 17.3 - Describe the pros and cons of RNA interference and...Ch. 17.4 - Explain how the universal nature of the genetic...Ch. 17.4 - Compare and contrast knockout, knockin, and...
Ch. 17.4 - Prob. 3LOCh. 17.5 - Describe the benefits of biofuel production from...Ch. 17.5 - Prob. 2LOCh. 17.5 - Prob. 3LOCh. 17.6 - Prob. 1LOCh. 17.6 - Compare and contrast FISH and gene chip...Ch. 17.6 - Describe how immunoassays can be used to diagnose...Ch. 17.7 - Describe the benefits of creating transgenic...Ch. 17.7 - Prob. 2LOCh. 17.7 - Evaluate issues on each side of the transgenic...Ch. 17 - Prob. 1DACh. 17 - Prob. 2DACh. 17 - Prob. 1IQCh. 17 - Prob. 2IQCh. 17 - You study a gene known to be important in the...Ch. 17 - What is the basis of separation of different DNA...Ch. 17 - Prob. 3UCh. 17 - FISH analysis of a breast tumor biopsy for HER2...Ch. 17 - In terms of studying gene function, what is the...Ch. 17 - The Ti plasmid of Agrobacterium usually induces...Ch. 17 - Prob. 1ACh. 17 - Which of the following statements is accurate for...Ch. 17 - Prob. 3ACh. 17 - Many human proteins, such as hemoglobin, are only...Ch. 17 - Amyloid beta is a proteolytic product of a protein...
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- Site-directed mutagenesis can be used to: a. treat diseases by gene therapy. b. make single residue mutation of a protein. c. make human insulin for treatment of diabetes. d. determine protein structures. e. determine DNA sequencingarrow_forwardWhich of the following is an outcome that could be produced by the application of the biotechnology as described? A. using PCR to modify a crop plant for higher yields B. introducing a recombinant DNA plasmid into a cell to trigger RNAi C. identifying SNPs to match birth parents to an adopted child D. using RT-PCR to generate copies of a gene to be expressed for insulin production An SNP is found associated with individuals who have diabetes. Which statement is true? A. All individuals with the SNP will have diabetes. B. The SNP codes for a faulty protein that causes the individual to have diabetes. C. To identify the SNP in more individuals, they will need to have their genome sequenced. D. The SNP may be linked to a gene that contributes to diabetes.arrow_forwardA company that manufactures cleansing products has synthesized two new organic compounds , chemicals A and B, which improve the cleaning power of dishwashing detergents. To determine the mutagenic capacity of the chemicals , the company tested the effect of the chemicals on Salmonella cells requiring histidine to grow ( His- cells ). The table below shows the results of the test . A. Name the test used by the company . B. What does the test detect or estimates ? C. Which chemical (s) would you identify as containing a mutagen ? Why ? D. What is the purpose of test 1 ? Why are colonies detected in test 1? E. Which chemical ( s) would you identify as possible antimutagen ? Why ?arrow_forward
- A. In what year was the National Bioethics Advisory Council formed? B. The first mammal clone to be produced from an adult (somatic cell)?arrow_forwardA. What is the pathogen that is attacking bananas today?b. Why is this especially problematic in Africa? C. Why do we expect to lose the war with this pathogen?d. What is random mutagenesis?arrow_forwardDescribe What are the sticky ends of the restriction fragments? Select one: a. The surfaces of sticky ends contain matching base pairs, allowing fragments to splice. b. The surfaces of sticky ends have glue like substance that allow fragments to splice. c. The surfaces of sticky ends contain the exact same nucleotides, allowing fragments to bond. d. The surfaces of sticky ends have velcro like structure, allowing fragments to bond.arrow_forward
- Someone submits a saliva sample for a PCR test to determine whether he is infected withSARS-CoV-2, an RNA virus. The first step is to purify RNA from the sample. Next, the RNA isconverted into cDNA.A. Why is this conversion important B. What is the first enzyme required for this step? C. Does this enzyme require a primer? Briefly explain. D. Three days later, the person submits another sample. The qPCR data againindicates a positive result; however, the spike gene Ct value is now 33. Based on the PCRdata, are they at the beginning or end of a COVID viral infection? Explain how you made thisdeterminationarrow_forwardSuppose we want to insert the human insulin gene into E. coli using pBR322 as the expression vector. 1. Explain how you will identify colonies that have been successfully transformed. 2. Identify all restriction enzyme(s) that would be appropriate for inserting the gene into pBR322. 3. Explain why the enzyme(s) from #2 are appropriate. Think about the function of the sequences in the expression vector when you answer this question. 4. The plasmid map shows that amp and ori are oriented in one direction and tet is in the opposite direction. How is this possible? 5. Suppose you decide to run the DNA on a gel to check each step. Draw the gel that would result from the following: Lane 1 molecular weight marker Lane 2 undigested pBR322 Lane 3 pBR322 digested with your selected restriction enzyme (identify which enzyme) Lane 4 pBR322 digested with your selected restriction enzyme then ligated with human insulin genearrow_forwardAll of the following are true about the design of the Beadle and Tatum experiment (one gene one polypeptide) EXCEPT: A. Used X-rays to generate auxotrophs B. Depended on the use of the bacteria, N. crassa C. Examined the way in which amino acid biosynthesis occurs D. Added nutrient intermediates to rescue lethal mutations included the study of conditional mutants E. Led to the one-gene-one polypeptide hypothesis.arrow_forward
- What is a BLAST search? a. A mechanism for aligning consensus regions during wholegenome sequencing b. A search for similar gene sequences from other species c. A method of screening a DNA library d. A method for identifying ORFsarrow_forwardWhich of the following would be considered a transgenic organism? A. a human whose human blood-clotting gene was silenced B. a fern grown in cell culture from a single fern root cell C. a rat with rabbit hemoglobin genesarrow_forwardAnswer each of the following questions correctly. a. How PCR may be used for the detection of disease–causing pathogens in a population during COVID Pandemic. For example: It may be used to check if a patient has a COVID virus infection. b. How cloning and expression of certain genes allows for massive production of the desired product. For example: the cloning and expression of insulin in bacteria allows for the mass production of this necessary protein for use by diabetic patients.arrow_forward
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