BIOLOGY
12th Edition
ISBN: 9781260169614
Author: Raven
Publisher: RENT MCG
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Chapter 17, Problem 2DA
Summary Introduction
To determine: The number of spots that would be observed when human cells in the G2 phase of the cell cycle are subjected to the FISH analysis using the probe recognizing telomeric DNA.
Introduction: Fluorescent in situ hybridization (FISH) is used to identify gross chromosomal rearrangements. It is a molecular cytogenic technique in which fluorescent probes are used to detect DNA sequences.
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Students have asked these similar questions
MIosis
Method:
Observations of onion root tip squash.
Scan the microscope under the 10x objective. Look for the region that has large
nuclei relative to the size of the cell; among these cells will be found cells displaying
stages of mitosis. Switch to the 40X objective to make closer observations. Since
prophase and prometaphase are difficult to distinguish, classify all these cells as
prophase. Record your observations in the table provided.
Examples are shown in the figure below.
Interphase
Early Prophase
Mid Prophase
10
Metaphase
Anaphase
Telophase
Exercises:
1.
Find and draw a cell showing each stage of mitosis.
Interphase
Prophase
Metaphase
Anaphase
Telophase
, 2.
What is a distinguishing visible feature of each stage of mitosis?
Interphase:
Prophase:
Metaphase:
Anaphase:
Telophase:
Many of the cells of the root meristem are not undergoing mitosis; rather they
Based upon the interpretations
3.
are in a stage called
21
You view a mature, living plant cell such as an epidermal cell under the light microscope. You view the cell at sufficiently-high magnification and you have also stained the cell for chromatin. The cell has a nucleus, yet you cannot see chromosomes, at least in their typical ’sausage-formed’ shape as we all know them from textbooks. Why is that?
Below is an image of human cheek cells (animal cells).
1. Can you see the nuclei of human cheek cells? If you can
see the nuclei, then label a nucleus of one human cheek
cell.
2. Were the human cheek cells stained? How do you know?
Chapter 17 Solutions
BIOLOGY
Ch. 17.1 - Prob. 1LOCh. 17.1 - Prob. 2LOCh. 17.1 - Describe the construction and uses of recombinant...Ch. 17.2 - Relate the process of DNA replication to PCR.Ch. 17.2 - Compare and contrast PCR, RT-PCR, and quantitative...Ch. 17.3 - Prob. 1LOCh. 17.3 - Prob. 2LOCh. 17.3 - Describe the pros and cons of RNA interference and...Ch. 17.4 - Explain how the universal nature of the genetic...Ch. 17.4 - Compare and contrast knockout, knockin, and...
Ch. 17.4 - Prob. 3LOCh. 17.5 - Describe the benefits of biofuel production from...Ch. 17.5 - Prob. 2LOCh. 17.5 - Prob. 3LOCh. 17.6 - Prob. 1LOCh. 17.6 - Compare and contrast FISH and gene chip...Ch. 17.6 - Describe how immunoassays can be used to diagnose...Ch. 17.7 - Describe the benefits of creating transgenic...Ch. 17.7 - Prob. 2LOCh. 17.7 - Evaluate issues on each side of the transgenic...Ch. 17 - Prob. 1DACh. 17 - Prob. 2DACh. 17 - Prob. 1IQCh. 17 - Prob. 2IQCh. 17 - You study a gene known to be important in the...Ch. 17 - What is the basis of separation of different DNA...Ch. 17 - Prob. 3UCh. 17 - FISH analysis of a breast tumor biopsy for HER2...Ch. 17 - In terms of studying gene function, what is the...Ch. 17 - The Ti plasmid of Agrobacterium usually induces...Ch. 17 - Prob. 1ACh. 17 - Which of the following statements is accurate for...Ch. 17 - Prob. 3ACh. 17 - Many human proteins, such as hemoglobin, are only...Ch. 17 - Amyloid beta is a proteolytic product of a protein...
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- What is different about mitosis in the plant cell as compared to the animal cell? Give two differences. please explain and do not copy from googlearrow_forwardBelgy Name: Date: Period: Mitosis Worksheet The diagram below shows six cells in various phases of the cell cycle. Note the cells are not arranged in the order in which mitosis occurs and one of the phases of mitosis occurs twice. Use the diagram to answer questions 1-7. Phases of the Cell Cycle 1) Cells A and early stage of the same phase of mitosis. What phase is it? 2) Which cell is in mg 3) Which cell rst phas sis? 4) In cell A, what structure is labeled X? 5) Which cell is in the "in between" phase of mitosis? 6) Place the diagrams in order from first to last. 7) Are the cells depicted plant animal cells? Explain your answer. 8) What is the longest phase of the cell cycle? 9) Why is mitosis important?arrow_forwardWhat is different about mitosis in the plant cell as compared to the animal cell? Give two differences. please do not copy from google and explain wellarrow_forward
- The drug taxol is extracted from the bark of yew trees. It binds tightly to microtubules and stabilizes them. When added to cells, it causes much of the free tubulin to assemble into microtubules. Taxol can be used as an anticancer drug. At the molecular level, what does the drug prevent? At the cellular level, what part of cell division does it prevent? Be specific.arrow_forward(a)arrow_forwardScientists often extract DNA from the nucleus of cells for analysis or use. This process breaks the cell membrane, spins the solution to remove the large particles through high g-force, adds alcohol to make the DNA less soluble aqueous solution, then spins the solution again to pull the DNA out of solution. Consider a cell in metaphase compared to a cell in rest (not in the cell cycle leading to cell division). What properties of a metaphase cell might let you extract more DNA compared to the resting cell? Are there any that might make the extraction more difficult?arrow_forward
- In the micrograph below, you are seeing a collection of cells that have been stained with fluorescent dyes. One of the dyes appears green, and the other dye appears blue (when viewed with a fluorescence microscope). Based on the appearance of these stained cells, which of the following cell structures must the GREEN dye be staining? Endoplasmic reticulum Cytoskeleton Flagella Mitochondria Nucleus ..arrow_forwardTELOPHASE OF ANIMAL CELL describe the appearance of DNA, spindle fibers and location of the chromosomes please describe the appearance and location, do not state the process do not copy from google please 4Xarrow_forwardTELOPHASE OF ANIMAL CELL describe the appearance of DNA, spindle fibers and location of the chromosomes please describe the appearance and location, do not state the process do not copy from google pleasearrow_forward
- Part A. Figure 1. Cells in various stages of mitotic division of an onion root tip.arrow_forwardThe image below is an Allium root tip under the microscope. Which statement describes what we are seeing? -dozens of cells in different parts of the cell cycle, mostly interphase -dozens of cells in different parts of the cell cycle, mostly telophase -dozens of cells in meiosis -one cell with many many nuclei dividingarrow_forwardHow long does it take for a cell to take one complete cycle? The answer varies on the type of cell and the species. For instance, a liver cell completes its cell cycle for over 22 hours (Becker and Lane 1965) while other somatic cell lasts to about 18 hours. Can you cite other types of cells that undergo cell cycle and the time it takes to complete the cycle? List five below.arrow_forward
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