Prescott's Microbiology
11th Edition
ISBN: 9781260211887
Author: WILLEY, Sandman, Wood
Publisher: McGraw Hill
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Chapter 17, Problem 3AL
Suppose you transformed a plasmid vector carrying a human interferon gene into E. coli but none of the transformed bacteria produced interferon. Give as many plausible reasons as possible for this result.
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(there are multiple right answers to this homework question but I can't tell which one is right)
You are designing a phage therapy for a cystic fibrosis patient with an multi-antibiotic resistant Mycobacterium infection. The Mycobacterium infection is preventing the patient from taking immunosuppressant drugs that are needed for a successful lung transplant. The idea is that introducing the right phage to the patient will kill the Mycobacterium cells and allow the patient to go on immunosuppressant drugs that will ensure the new lung is not rejected by the body.
In the first step of your phage therapy design process, you isolate the exact Mycobacterium strain from the patient. What is the most important thing must you establish next about this bacterial isolate?
That the isolate is genomically stable so you can study it
That the isolate is similar to Mycobacterium in other patients
That the isolate rapidly evolves due to horizontal gene transfer
That the Mycobacterium is…
You are designing a phage therapy for a cystic fibrosis patient with an multi-antibiotic resistant Mycobacterium infection. The Mycobacterium infection is preventing the patient from taking immunosuppressant drugs that are needed for a successful lung transplant. The idea is that introducing the right phage to the patient will kill the Mycobacterium cells and allow the patient to go on immunosuppressant drugs that will ensure the new lung is not rejected by the body.
You need to find a phage that infects the Mycobacterium you isolated from the patient.
Which TWO options have the highest chance of success?
A. Screening a colleague's library of known Mycobacterium phages for phage that infect the patient's Mycobacterium isolate
b. Choosing likely candidates from analysis of phage 16S rRNA phylogeny in the tree of life
C. Isolating new phage from soil using a plaque assay with the Mycobacterium isolate
D. Obtaining a very well-studied phage, such as T4 bacteriophage, that…
You are designing a phage therapy for a cystic fibrosis patient with an multi-antibiotic resistant Mycobacterium infection. The Mycobacterium infection is preventing the patient from taking immunosuppressant drugs that are needed for a successful lung transplant. The idea is that introducing the right phage to the patient will kill the Mycobacterium cells and allow the patient to go on immunosuppressant drugs that will ensure the new lung is not rejected by the body.
You finally find a phage that you think will work because it attaches to and injects its genome into the patient's Mycobacterium isolate. Next, you need to be sure of what characteristics of the phage infection cycle? Pick all that are true
A. That the phage delivers new antibiotic resistance genes to Mycobacterium via transduction
B. That the correct proteins are expressed during the phages metabolism
C. That the infection is lytic
D.That lysogeny is the primary infection pathway
Chapter 17 Solutions
Prescott's Microbiology
Ch. 17.1 - Examine the uncut piece of DNA shown in the upper...Ch. 17.1 - Which of the above enzymes yield blunt ends? Which...Ch. 17.1 - Prob. 3MICh. 17.1 - What would you conclude if you obtained only blue...Ch. 17.1 - Why must introns be removed from eukaryotic DNA...Ch. 17.1 - Which plasmid is a shuttle vector? Why?Ch. 17.1 - In what ways does the BAC shown here differ from...Ch. 17.1 - Describe restriction enzymes, sticky ends, and...Ch. 17.1 - What is cDNA? Why is it necessary to generate cDNA...Ch. 17.1 - Prob. 3CC
Ch. 17.1 - Prob. 4CCCh. 17.1 - Prob. 5CCCh. 17.2 - Why, after three cycles, are the vast majority of...Ch. 17.2 - Briefly describe the polymerase chain reaction....Ch. 17.2 - Why is PCR used to detect infectious agents that...Ch. 17.2 - How would you use PCR to measure the concentration...Ch. 17.2 - Why is it possible to visualize a PCR product on...Ch. 17.2 - Prob. 5CCCh. 17.3 - Why are long fragments (e.g., 20,000 bp) of...Ch. 17.4 - What special considerations are necessary if one...Ch. 17.4 - Prob. 1CCCh. 17.4 - Prob. 2CCCh. 17.4 - Prob. 3CCCh. 17.4 - You are studying chemotaxis proteins in a newly...Ch. 17.5 - Prob. 1MICh. 17.5 - Prob. 1CCCh. 17.5 - Prob. 2CCCh. 17 - Which of the DNA molecules shown are recombinant?Ch. 17 - Prob. 1RCCh. 17 - Prob. 2RCCh. 17 - Prob. 3RCCh. 17 - Prob. 4RCCh. 17 - Prob. 5RCCh. 17 - Prob. 6RCCh. 17 - Prob. 1ALCh. 17 - Prob. 2ALCh. 17 - Suppose you transformed a plasmid vector carrying...Ch. 17 - You are interested in the activity and regulation...Ch. 17 - Prob. 5ALCh. 17 - Prob. 6ALCh. 17 - Prob. 7AL
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- True or False: Plasmids can ONLY be used in transforming bacterial cells.arrow_forwardSome strains of Escherichia coli bacteria have acquired the ability to produce the harmful Shiga toxin, normally produced by Shigella dysenteriae. Which statements best explain why this occurs? Mark all that apply. A. conjugation between two different species of bacteria allowed both strains of bacteria to express a virulence factor B. transformation resulted in expression of a virulence factor C. horizontal gene transfer between different species of bacteria D. transduction of a specific gene resulted in expression of new traitsarrow_forwardWhich of the following apply to the development of urogenital tract Candida albicans infections? Disruption of population dynamics allowing the RB form of Chlamydia to cause tissue damage Loss of Lactobacillus populations leading to an increase in the local pH Loss of Gardnerella vaginalis populations leading to a lose of Clue cells Loss of the anaerobic environment that promotes the outgrowth of Trichomonas vaginalis None of the answers applyarrow_forward
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