Prescott's Microbiology
11th Edition
ISBN: 9781260211887
Author: WILLEY, Sandman, Wood
Publisher: McGraw Hill
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Chapter 17, Problem 3AL
Suppose you transformed a plasmid vector carrying a human interferon gene into E. coli but none of the transformed bacteria produced interferon. Give as many plausible reasons as possible for this result.
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You want to product human immunoglobulin G using a recombinant Escherichia coli system. Design the whole procedure and strategy by yourself.
In Hershey-Chase experiment, bacteriophages protein coats were tagged with radioactive isotope S-32. These phages were used to infect E. coli cells and the cells were further centrifuged to form pellets.
Why was the radioactivity level of S-32 found greater outside the cells compared to the E. coli cell pellets? Explain briefly.
If the experiment is repeated in the same manner but this time the phage protein coats are labelled with isotope X and the phage DNA with isotope Y, which isotope’s radioactivity will be found in greater amounts in the E. coli cell pellets after centrifugation? Explain briefly.
The figure above shows a schematic of genes and transcription control elements from phage λ. Use this figure as an aid to help you describe the molecular events involved in:
a) The establishment of lysogeny
b) The establishment of a lytic life cycle
Chapter 17 Solutions
Prescott's Microbiology
Ch. 17.1 - Examine the uncut piece of DNA shown in the upper...Ch. 17.1 - Which of the above enzymes yield blunt ends? Which...Ch. 17.1 - Prob. 3MICh. 17.1 - What would you conclude if you obtained only blue...Ch. 17.1 - Why must introns be removed from eukaryotic DNA...Ch. 17.1 - Which plasmid is a shuttle vector? Why?Ch. 17.1 - In what ways does the BAC shown here differ from...Ch. 17.1 - Describe restriction enzymes, sticky ends, and...Ch. 17.1 - What is cDNA? Why is it necessary to generate cDNA...Ch. 17.1 - Prob. 3CC
Ch. 17.1 - Prob. 4CCCh. 17.1 - Prob. 5CCCh. 17.2 - Why, after three cycles, are the vast majority of...Ch. 17.2 - Briefly describe the polymerase chain reaction....Ch. 17.2 - Why is PCR used to detect infectious agents that...Ch. 17.2 - How would you use PCR to measure the concentration...Ch. 17.2 - Why is it possible to visualize a PCR product on...Ch. 17.2 - Prob. 5CCCh. 17.3 - Why are long fragments (e.g., 20,000 bp) of...Ch. 17.4 - What special considerations are necessary if one...Ch. 17.4 - Prob. 1CCCh. 17.4 - Prob. 2CCCh. 17.4 - Prob. 3CCCh. 17.4 - You are studying chemotaxis proteins in a newly...Ch. 17.5 - Prob. 1MICh. 17.5 - Prob. 1CCCh. 17.5 - Prob. 2CCCh. 17 - Which of the DNA molecules shown are recombinant?Ch. 17 - Prob. 1RCCh. 17 - Prob. 2RCCh. 17 - Prob. 3RCCh. 17 - Prob. 4RCCh. 17 - Prob. 5RCCh. 17 - Prob. 6RCCh. 17 - Prob. 1ALCh. 17 - Prob. 2ALCh. 17 - Suppose you transformed a plasmid vector carrying...Ch. 17 - You are interested in the activity and regulation...Ch. 17 - Prob. 5ALCh. 17 - Prob. 6ALCh. 17 - Prob. 7AL
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Bacteria exposed to viruses incorporate sections of the virus’s DNA into the CRISPR array sequences in their genome. This mechanism allows bacteria to fight off the viruses, like an immune response: the information in CRISPR spacers served as “coordinates” for recognizing and cutting up invading DNA sequences. Describe what might happen under the conditions described after a bacteriophage infects a bacterial cell and releases its DNA into the bacterial cell. Explain why: 1. The invading phage DNA is recognized by the Cas proteins but not inserted into the CRISPR array region of the bacterial genome: The bacteria will be unable to elicit an immune response and will succumb to the phase infection 2. The cas genes on the bacterial genome contains a missense mutation that increases its cleavage/cut activityThe bacteria will elicit an immune response that will successfully fight the phage infectionarrow_forwardYou were able to obtain the RNA of the influenza virus. If you injected this into a susceptible host cell could this RNA alone cause a lytic infection? Please explain why or why not with proper reasoning.arrow_forwardWhen various strains of λ phage are seeded on a lawn of E. coli, they can form clear or turbid plaques. (b) For mutant λ phages that can only form clear plaques, give two different types of mutation in the phage that can explain the clear plaque phenotype.arrow_forward
- Why are bacterial DNA gyrase and topoisomerase good targets for antimicrobial drugs? These enzymes are not necessary for DNA replication. These enzymes are involved in energy production, so targeting them would prevent the bacteria from obtaining necessary energy. These enzymes are different from their eukaryotic counterparts, so the drugs targeting them should not affect the host. These enzymes are responsible for host cell recognition, so targeting them would prevent the host cell from being infected.Flag this QuestionQuestion 10arrow_forwardSome strains of Escherichia coli bacteria have acquired the ability to produce the harmful Shiga toxin, normally produced by Shigella dysenteriae. Which statements best explain why this occurs? Mark all that apply. A. conjugation between two different species of bacteria allowed both strains of bacteria to express a virulence factor B. transformation resulted in expression of a virulence factor C. horizontal gene transfer between different species of bacteria D. transduction of a specific gene resulted in expression of new traitsarrow_forwardWhich of the following apply to the development of urogenital tract Candida albicans infections? Disruption of population dynamics allowing the RB form of Chlamydia to cause tissue damage Loss of Lactobacillus populations leading to an increase in the local pH Loss of Gardnerella vaginalis populations leading to a lose of Clue cells Loss of the anaerobic environment that promotes the outgrowth of Trichomonas vaginalis None of the answers applyarrow_forward
- The size of a DNA fragment that can be inserted into an unmodified λ vector is very limited. Large segment in the central region of the λ DNA molecule can be removed without affecting the ability of the phage to infect Escherichia coli cells. However, the deletion of this non- essential region does affect the life cycle of the bacteriophage. Explain this statement.arrow_forwardWhich statement describes the difference between a lytic and lysogenic phage cycle? In a lytic cycle, new phage particles will be produced; in a lysogenic cycle, the phage DNA is passed to new cells but does not produce phage particles. In a lytic cycle, the host cell does not die; in a lysogenic cycle, the host is killed. In a lytic cycle, the phage DNA enters the host cell; in a lysogenic cycle, the phage DNA remains on the exterior of the host cell. In a lytic cycle, the phage DNA incorporates into the host genome; in a lysogenic cycle, the phage DNA does not incorporate into the host genome.arrow_forwardTrue or False: Plasmids can ONLY be used in transforming bacterial cells.arrow_forward
- In order to determine the genetic material of a T2 phage, Alfred Hershey and Martha Chase conducted experiments using T2 phages that infected bacteria. In one treatment, they grew phages with radioactive sulfur. In another treatment, they grew phages with radioactive phosphorous. They allowed both types of phages to infect bacterial cells. After infection, they found that only bacteria infected with phages grown with radioactive phosphorous showed any radioactivity. Why did they use radioactive sulfur and phosphorous for this experiment? * O Sulfur is part of the DNA molecule but not part of a protein molecule. Sulfur and phosphorous are some of the most reactive molecules and are easily traced. Sulfur and phosphorous are able to survive the centrifuge, a crucial component of the experiment. O Phosphorous is part of the DNA molecule but not part of a protein molecule.arrow_forwardIn a petri dish with solidified agar with escherichia coli, enterobacter aerogenes and staphylococcus aureus, you streak a loopfull of lytic T4-phage in a single line onto the center of the the dish, how do you know if bacteriophage infected the bacteria. why didn't the bacteriophage infect all 3 bacteria?arrow_forwardDuring the adenoviral SARS-CoV2 vaccine development process, the plasmid encoding the Spike protein is generated. Elaborate on the steps you would follow to generate a recombinant plasmid encoding the Spike protein.arrow_forward
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