The approximate size of the DNA fragments in lanes 1,2 and 3 using the standards given in lane 4. Introduction: DNA fragmentation involves the separation or the breaking of the DNA strands into small fragments or pieces. It can occur spontaneously or can be induced deliberately in laboratories.

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Answer 1

Question

Chapter 17.1, Problem 3MI

Summary Introduction

To determine: The approximate size of the DNA fragments in lanes 1,2 and 3 using the standards given in lane 4.

Introduction: DNA fragmentation involves the separation or the breaking of the DNA strands into small fragments or pieces. It can occur spontaneously or can be induced deliberately in laboratories.

Summary Introduction

To determine: The digestion of the DNA strand in lane 1 does not allow the loss of any DNA.

Introduction: Agarose and Polyacrylamide Gel Electrophoresis are the two methods which are used to routinely separate and produce DNA fragments of variable size.

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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results? 2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab? 3) Why are the cells incubated at 42°C?

Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…

Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…

Answer 2

Question

Chapter 17.1, Problem 3MI

Summary Introduction

To determine: The approximate size of the DNA fragments in lanes 1,2 and 3 using the standards given in lane 4.

Introduction: DNA fragmentation involves the separation or the breaking of the DNA strands into small fragments or pieces. It can occur spontaneously or can be induced deliberately in laboratories.

Summary Introduction

To determine: The digestion of the DNA strand in lane 1 does not allow the loss of any DNA.

Introduction: Agarose and Polyacrylamide Gel Electrophoresis are the two methods which are used to routinely separate and produce DNA fragments of variable size.

Expert Solution & Answer

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Answer 3

Question

Chapter 17.1, Problem 3MI

Summary Introduction

To determine: The approximate size of the DNA fragments in lanes 1,2 and 3 using the standards given in lane 4.

Introduction: DNA fragmentation involves the separation or the breaking of the DNA strands into small fragments or pieces. It can occur spontaneously or can be induced deliberately in laboratories.

Summary Introduction

To determine: The digestion of the DNA strand in lane 1 does not allow the loss of any DNA.

Introduction: Agarose and Polyacrylamide Gel Electrophoresis are the two methods which are used to routinely separate and produce DNA fragments of variable size.

Expert Solution & Answer

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Answer 4

Question

Chapter 17.1, Problem 3MI

Summary Introduction

To determine: The approximate size of the DNA fragments in lanes 1,2 and 3 using the standards given in lane 4.

Introduction: DNA fragmentation involves the separation or the breaking of the DNA strands into small fragments or pieces. It can occur spontaneously or can be induced deliberately in laboratories.

Summary Introduction

To determine: The digestion of the DNA strand in lane 1 does not allow the loss of any DNA.

Introduction: Agarose and Polyacrylamide Gel Electrophoresis are the two methods which are used to routinely separate and produce DNA fragments of variable size.

Expert Solution & Answer

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Answer 5

Chapter 17.1, Problem 3MI

Summary Introduction

To determine: The approximate size of the DNA fragments in lanes 1,2 and 3 using the standards given in lane 4.

Introduction: DNA fragmentation involves the separation or the breaking of the DNA strands into small fragments or pieces. It can occur spontaneously or can be induced deliberately in laboratories.

Summary Introduction

To determine: The digestion of the DNA strand in lane 1 does not allow the loss of any DNA.

Introduction: Agarose and Polyacrylamide Gel Electrophoresis are the two methods which are used to routinely separate and produce DNA fragments of variable size.

Answer 6

Chapter 17.1, Problem 3MI

Summary Introduction

To determine: The approximate size of the DNA fragments in lanes 1,2 and 3 using the standards given in lane 4.

Introduction: DNA fragmentation involves the separation or the breaking of the DNA strands into small fragments or pieces. It can occur spontaneously or can be induced deliberately in laboratories.

Answer 7

Chapter 17.1, Problem 3MI

Summary Introduction

To determine: The approximate size of the DNA fragments in lanes 1,2 and 3 using the standards given in lane 4.

Introduction: DNA fragmentation involves the separation or the breaking of the DNA strands into small fragments or pieces. It can occur spontaneously or can be induced deliberately in laboratories.

Answer 8

Summary Introduction

To determine: The digestion of the DNA strand in lane 1 does not allow the loss of any DNA.

Introduction: Agarose and Polyacrylamide Gel Electrophoresis are the two methods which are used to routinely separate and produce DNA fragments of variable size.

Answer 9

Summary Introduction

To determine: The digestion of the DNA strand in lane 1 does not allow the loss of any DNA.

Introduction: Agarose and Polyacrylamide Gel Electrophoresis are the two methods which are used to routinely separate and produce DNA fragments of variable size.

Answer 10

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Answer 11

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Answer 12

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Answer 13

Ch. 17.1 - Examine the uncut piece of DNA shown in the upper...Ch. 17.1 - Which of the above enzymes yield blunt ends? Which...Ch. 17.1 - Prob. 3MI Ch. 17.1 - What would you conclude if you obtained only blue...Ch. 17.1 - Why must introns be removed from eukaryotic DNA...Ch. 17.1 - Which plasmid is a shuttle vector? Why?Ch. 17.1 - In what ways does the BAC shown here differ from...Ch. 17.1 - Describe restriction enzymes, sticky ends, and...Ch. 17.1 - What is cDNA? Why is it necessary to generate cDNA...Ch. 17.1 - Prob. 3CC

The approximate size of the DNA fragments in lanes 1,2 and 3 using the standards given in lane 4. Introduction: DNA fragmentation involves the separation or the breaking of the DNA strands into small fragments or pieces. It can occur spontaneously or can be induced deliberately in laboratories.

To determine: The digestion of the DNA strand in lane 1 does not allow the loss of any DNA. Introduction: Agarose and Polyacrylamide Gel Electrophoresis are the two methods which are used to routinely separate and produce DNA fragments of variable size.

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Chapter 17 Solutions

To determine: The digestion of the DNA strand in lane 1 does not allow the loss of any DNA.

Introduction: Agarose and Polyacrylamide Gel Electrophoresis are the two methods which are used to routinely separate and produce DNA fragments of variable size.