Which of the following would be considered a transgenic organism? A. a human whose human blood-clotting gene was silenced B. a fern grown in cell culture from a single fern root cell C. a rat with rabbit hemoglobin genes
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- Which of the following is an outcome that could be produced by the application of the biotechnology as described? A. using PCR to modify a crop plant for higher yields B. introducing a recombinant DNA plasmid into a cell to trigger RNAi C. identifying SNPs to match birth parents to an adopted child D. using RT-PCR to generate copies of a gene to be expressed for insulin production An SNP is found associated with individuals who have diabetes. Which statement is true? A. All individuals with the SNP will have diabetes. B. The SNP codes for a faulty protein that causes the individual to have diabetes. C. To identify the SNP in more individuals, they will need to have their genome sequenced. D. The SNP may be linked to a gene that contributes to diabetes.Explain the difference between gene modification and gene addition.Are the following examples of gene modification or gene addition?A. A mouse model to study cystic fibrosisB. Introduction of a pesticide-resistance gene into corn using theT-DNA vector of A. tumefaciensWhich of the following is transgenic? a. A yeast cell that expresses a gene from a fish b. A human patient who receives gene therapy to repair a faulty cystic fibrosis gene c. A human embryo being tested for disease-causing alleles d. All of the above are transgenic.
- What is a genetically modified organism (GMO)? a. a plant with certain genes removed b. an organism with an artificially altered genome c. a hybrid organism d. any agricultural organism produced by breeding or biotechnologyWhat is the role of Agrobacterium tumefaciens in the production of transgenic plants? a. Genes from A. fume fociens are inserted into plant DNA to give the plant different traits. b. Transgenic plants have been given resistance to the pest A. tumefacaens. c. A. wmefaciens is used as a vector to move genes into plant cells. d. Plant genes are incorporated into the genome of Agrobacterium tumefaciens.What is the proper order of the following steps in a gene-cloningexperiment involving vectors?1. Add DNA ligase.2. Incubate the chromosomal DNA and the vector DNA with arestriction enzyme.3. Introduce the DNA into living cells.4. Mix the chromosomal DNA and vector DNA together.a. 1, 2, 3, 4b. 2, 3, 1, 4c. 2, 4, 1, 3d. 1, 2, 4, 3
- When A. tumefaciens is used to make a transgenic plant, agene of interest is inserted into a _________, which is firsttransformed into __________. The __________ is then transferredto a plant.a. viral vector, E. coli, gene of interestb. T-DNA vector, A. tumefaciens, T DNA carrying the gene ofinterestc. T-DNA vector, A. tumefaciens, gene of interest aloned. T-DNA vector, E. coli, gene of interest aloneWhich of the following is an outcome that could be produced by the application of the biotechnology as described? A. using PCR to modify a crop plant for higher yields B. introducing a recombinant DNA plasmid into a cell to trigger RNAi C. identifying SNPs to match birth parents to an adopted child D. using RT-PCR to generate copies of a gene to be expressed for insulin productionWhich of the following can appropriately be described as a transgenic organism?A. The sheep Dolly, which was produced by cloningB. A sheep that produces human α1-antitrypsin in its milkC. Bt cornD. A hybrid strain of corn produced from crossing two inbred strainsof corn (which were not transgenic)
- Genetically engineered human insulin, human growth hormone, and human clotting factor VIII are made by:a. gel electrophoresis b. polymerase chain reactionc. transgenic bacteriad. DNA fingerprintingMatch each word with the best description. DNA profile Ti plasmid probes SNPs transgenic genomics CRISPR a. alleles commonly have them b. mouse vs. human c. based on RNA interference d. used in plant gene transfers e. unique array of short tandem repeats f.…One concern about using genetically-modified organisms is that many of the methods used to create them introduce into the genome DNA from a different species (i.e. foreign DNA). Which of the following methods has the lowest potential of introducing foreign DNA into the genome?A. A gene knockout in mouse using homologous recombination in ES cellsB. Introduction of a P element vector into the Drosophila germ-lineC. Deletion mutations introduced by CRISPR/Cas9D. Microinjection of a transgene into a mouse pronucleus