a. To determine: The DNA element, along with its location, which includes expressing the GFP protein in Drosophila . Also, the ways in which one can make a certain conclusion that GFP is expressed only in wings. Introduction: A promoter is a region of DNA which initiates transcription of that gene. They are located near the transcription initiation sites of genes. They are present on the same strands and upstream of the DNA. They are nearly 100-1000 base pairs long.

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Answer 1

Question

Chapter 17, Problem 12P

Summary Introduction

a.

To determine:

The DNA element, along with its location, which includes expressing the GFP protein in Drosophila. Also, the ways in which one can make a certain conclusion that GFP is expressed only in wings.

Introduction:

A promoter is a region of DNA which initiates transcription of that gene. They are located near the transcription initiation sites of genes. They are present on the same strands and upstream of the DNA. They are nearly 100-1000 base pairs long.

Summary Introduction

b.

To determine:

The application of GFP fluorescence in different strains along with an explanation of these results.

Introduction:

An enhancer is a short region of around 50-1500bp region of DNA. An enhancer is bound to activators, which increase the rate of transcription of that particular gene. The enhancer proteins are also called transcription factors.

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Protein levels and mRNA levels for a particualr gene don’t always match. For example, the GCN4 gene in yeast is always producing mRNA, but the Gcn4 protein is only made when the cells are starved. A. what is one mechanism by which changes in mRNA levels are not always matched by changes in the protein product of that mRNA?

a. How do bacteria increase the efficiency of gene expression? Is this possible in eukaryotes? b. A mutation in the promoter of Gene K disrupts an enzyme binding site and results in the loss of Gene K expression. Is this change in gene expression likely happening at the transcriptional or the translational level? Explain. c. Propose three different mutations to prevent initiation, elongation, and termination of bacterial transcription, respectively. Explain how/why each mutation would prevent its respective step. (Hint: mutations can be in genes that encode proteins or regulatory DNA sequences)

a. The eyeless gene is required for eye formation in Drosophila. It encodes a homeodomain. What would you predict about the biochemical function of the Eyeless protein?b. Where would you predict that the eyeless gene is expressed in development? How would you test your prediction? c. The Small eye and Aniridia genes of mice and humans, respectively, encode proteins with very strong sequence similarity to the fly Eyeless protein, and they are named for their effects on eye development. Devise one test to examine whether the mouse and human genes are functionally equivalent to the fly eyeless gene.

Answer 2

Question

Chapter 17, Problem 12P

Summary Introduction

a.

To determine:

The DNA element, along with its location, which includes expressing the GFP protein in Drosophila. Also, the ways in which one can make a certain conclusion that GFP is expressed only in wings.

Introduction:

A promoter is a region of DNA which initiates transcription of that gene. They are located near the transcription initiation sites of genes. They are present on the same strands and upstream of the DNA. They are nearly 100-1000 base pairs long.

Summary Introduction

b.

To determine:

The application of GFP fluorescence in different strains along with an explanation of these results.

Introduction:

An enhancer is a short region of around 50-1500bp region of DNA. An enhancer is bound to activators, which increase the rate of transcription of that particular gene. The enhancer proteins are also called transcription factors.

Expert Solution & Answer

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Answer 3

Question

Chapter 17, Problem 12P

Summary Introduction

a.

To determine:

The DNA element, along with its location, which includes expressing the GFP protein in Drosophila. Also, the ways in which one can make a certain conclusion that GFP is expressed only in wings.

Introduction:

A promoter is a region of DNA which initiates transcription of that gene. They are located near the transcription initiation sites of genes. They are present on the same strands and upstream of the DNA. They are nearly 100-1000 base pairs long.

Summary Introduction

b.

To determine:

The application of GFP fluorescence in different strains along with an explanation of these results.

Introduction:

An enhancer is a short region of around 50-1500bp region of DNA. An enhancer is bound to activators, which increase the rate of transcription of that particular gene. The enhancer proteins are also called transcription factors.

Expert Solution & Answer

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See solution

Answer 4

Question

Chapter 17, Problem 12P

Summary Introduction

a.

To determine:

The DNA element, along with its location, which includes expressing the GFP protein in Drosophila. Also, the ways in which one can make a certain conclusion that GFP is expressed only in wings.

Introduction:

A promoter is a region of DNA which initiates transcription of that gene. They are located near the transcription initiation sites of genes. They are present on the same strands and upstream of the DNA. They are nearly 100-1000 base pairs long.

Summary Introduction

b.

To determine:

The application of GFP fluorescence in different strains along with an explanation of these results.

Introduction:

An enhancer is a short region of around 50-1500bp region of DNA. An enhancer is bound to activators, which increase the rate of transcription of that particular gene. The enhancer proteins are also called transcription factors.

Expert Solution & Answer

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Answer 5

Chapter 17, Problem 12P

Summary Introduction

a.

To determine:

The DNA element, along with its location, which includes expressing the GFP protein in Drosophila. Also, the ways in which one can make a certain conclusion that GFP is expressed only in wings.

Introduction:

A promoter is a region of DNA which initiates transcription of that gene. They are located near the transcription initiation sites of genes. They are present on the same strands and upstream of the DNA. They are nearly 100-1000 base pairs long.

Summary Introduction

b.

To determine:

The application of GFP fluorescence in different strains along with an explanation of these results.

Introduction:

An enhancer is a short region of around 50-1500bp region of DNA. An enhancer is bound to activators, which increase the rate of transcription of that particular gene. The enhancer proteins are also called transcription factors.

Answer 6

Chapter 17, Problem 12P

Summary Introduction

a.

To determine:

The DNA element, along with its location, which includes expressing the GFP protein in Drosophila. Also, the ways in which one can make a certain conclusion that GFP is expressed only in wings.

Introduction:

A promoter is a region of DNA which initiates transcription of that gene. They are located near the transcription initiation sites of genes. They are present on the same strands and upstream of the DNA. They are nearly 100-1000 base pairs long.

Answer 7

Chapter 17, Problem 12P

Summary Introduction

a.

To determine:

The DNA element, along with its location, which includes expressing the GFP protein in Drosophila. Also, the ways in which one can make a certain conclusion that GFP is expressed only in wings.

Introduction:

A promoter is a region of DNA which initiates transcription of that gene. They are located near the transcription initiation sites of genes. They are present on the same strands and upstream of the DNA. They are nearly 100-1000 base pairs long.

Answer 8

Summary Introduction

b.

To determine:

The application of GFP fluorescence in different strains along with an explanation of these results.

Introduction:

An enhancer is a short region of around 50-1500bp region of DNA. An enhancer is bound to activators, which increase the rate of transcription of that particular gene. The enhancer proteins are also called transcription factors.

Answer 9

Summary Introduction

b.

To determine:

The application of GFP fluorescence in different strains along with an explanation of these results.

Introduction:

An enhancer is a short region of around 50-1500bp region of DNA. An enhancer is bound to activators, which increase the rate of transcription of that particular gene. The enhancer proteins are also called transcription factors.

Answer 10

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Answer 11

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Answer 12

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Answer 13

Ch. 17 - For each of the terms in the left column, choose...Ch. 17 - For each of the following types of gene...Ch. 17 - List five events other than transcription...Ch. 17 - Which eukaryotic RNA polymerase RNA pol I, pol II,...Ch. 17 - As shown in the following diagram, a single...Ch. 17 - You have synthesized an enhancerless GFP reporter...Ch. 17 - Prob. 7P Ch. 17 - Prob. 8P Ch. 17 - A single UAS regulates the expression of three...Ch. 17 - MyoD is a transcriptional activator that turns on...

Solution Summary: The author explains how a construct into the genome of Drosophila requires DNA element and promoter to express green fluorescent protein (GFP) in flies.

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