Prescott's Microbiology
11th Edition
ISBN: 9781260211887
Author: WILLEY, Sandman, Wood
Publisher: McGraw Hill
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Textbook Question
Chapter 16.7, Problem 3CC
Discuss two ways in which artificial transformation can be used to place functional genes within bacterial cells.
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Chapter 16 Solutions
Prescott's Microbiology
Ch. 16.1 - Retrieve, Infer, Apply List three ways in which...Ch. 16.1 - Compare and contrast the means by which the...Ch. 16.1 - Give examples of intragenic and extragenic...Ch. 16.1 - Retrieve, Infer, Apply Sometimes a point mutation...Ch. 16.1 - Retrieve, Infer, Apply Why might a missense...Ch. 16.2 - How would you screen for a tryptophan auxotroph?...Ch. 16.2 - Why is a small amount of histidine added to the...Ch. 16.2 - Retrieve, Infer, Apply Describe how replica...Ch. 16.2 - Retrieve, Infer, Apply Why are mutant selection...Ch. 16.2 - Retrieve, Infer, Apply Briefly discuss how...
Ch. 16.2 - Describe how you would isolate a mutant that...Ch. 16.2 - Prob. 5CCCh. 16.3 - How is mismatch repair similar to DNA polymerase...Ch. 16.3 - How is damaged DNA recognized by the UvrAB...Ch. 16.3 - Prob. 1CCCh. 16.3 - Retrieve, Infer, Apply What role does DNA...Ch. 16.3 - Retrieve, Infer, Apply When E. coli cells are...Ch. 16.3 - Explain how the following DNA alterations and...Ch. 16.4 - An antibiotic-resistance gene located on a...Ch. 16.4 - What four fates can DNA have after entering a...Ch. 16.4 - How does homologous recombination differ from...Ch. 16.5 - What features are common to all types of...Ch. 16.5 - How does a transposon differ from an insertion...Ch. 16.5 - What is simple (cut-and-paste) transposition? What...Ch. 16.5 - What effect would you expect the existence of...Ch. 16.6 - Prob. 1MICh. 16.6 - What is bacterial conjugation and how was it...Ch. 16.6 - For F+, Hfr, and F strains of E. coli, indicate...Ch. 16.6 - Describe how F+ F and Hfr conjugation processes...Ch. 16.6 - Compare and contract F+ F and F F conjugation.Ch. 16.7 - According to this model, what would happen if DNA...Ch. 16.7 - Prob. 1CCCh. 16.7 - Describe how transformation occurs in S....Ch. 16.7 - Discuss two ways in which artificial...Ch. 16.8 - Compare the number of transducing particles that...Ch. 16.8 - Why cant the gal and bio genes be transduced by...Ch. 16.8 - Describe generalized transduction and how it...Ch. 16.8 - What is specialized transduction and how does it...Ch. 16.8 - How might one tell whether horizontal gene...Ch. 16.8 - Why doesnt a cell lyse after successful...Ch. 16.8 - Describe how conjugation, transformation, and...Ch. 16.9 - As a replicative transposon, what would happen if...Ch. 16 - Prob. 1RCCh. 16 - Prob. 2RCCh. 16 - Prob. 3RCCh. 16 - Prob. 4RCCh. 16 - Prob. 5RCCh. 16 - Prob. 6RCCh. 16 - Mutations are often considered harmful. Give an...Ch. 16 - Mistakes made during transcription affect the cell...Ch. 16 - Suppose that transduction took place when a U-tube...Ch. 16 - Suppose that you carried out a U-tube experiment...Ch. 16 - Prob. 5ALCh. 16 - Prob. 6ALCh. 16 - Prob. 7AL
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- Cloning vectors are not just limited to bacterial plasmids. Bacteriophages and M13 phage vectors are also commonly utilized in the cloning process. State any five (5) key criteria to be an effective cloning vector.arrow_forwardWhat is vector? Discuss 2 vectors that are commonly used in DNA recombinant technology.arrow_forwardSite directed mutagenesis is used to: 1.determine the critical base per sequences in a Genome 2. Create genomic libraries 3. Determine the critical amino acids in a protein that allowed to function 4. Amplify dna 5. sequence dnaarrow_forward
- What are site-recombinases? Describe in detail how cre- recombinase can be used to decipher the roles of specific genes and proteins in complex multicellular organisms? Explain how the cre-lox system can be used to examine the role of a particular gene in a specific type of tissue?arrow_forwardHow can site-specific recombination be used in recombinant DNA technology? Explain with an examplearrow_forwardDescribe in vitro mutagenesis and its potential uses.arrow_forward
- a) What are vectors? Describe extensively the roles vectors play in genetic engineering? Write short notees on the following: Recombinant DNA, Cloning b) What are restriction enzymes? Describe extensively the roles restriction enzymes play in genetic engineering? Write short notees on the following: Selectable markers, Cloningarrow_forwardOutline a strategy for constructing a genomic DNA library more representative of the entire humangenome. You will need to consider alternative vectors and the efficiency of transformation of thebacterial cellsarrow_forwardWith the use of well-illustrated diagrams, reconstruct the entire cloning process by explaining different stages of the cloning process that involves the following: d. Screening/selection of hosts containing the intended recombinant plasmid. For this stage(d), discuss the importance of a second marker that can be used for screening of genomic DNA for colonies containing the pka-1 under the principle of insertional inactivation. This should be properly explained using all the attributes of the plasmid described above.arrow_forward
- Genetic transfer via transformation can also be used to map genes along the bacterial chromosome. In this approach, fragments of chromosomal DNA are isolated from one bacterial strain and used to transform another strain. The experimenter examines the transformed bacteria to see if they have incorporated two or more different genes. For example, the DNA may be isolated from a donor E. coli bacterium that has functional copies of the araB and leuD genes. Let’s call these genes araB+ and leuD+ to indicate the genes are functional. These two genes are required for arabinose metabolismand leucine synthesis, respectively. To map the distance betweenthese two genes via transformation, a recipient bacterium is used that is araB− and leuD−. Following transformation, the recipient bacterium may become araB+ and leuD+. This phenomenon is calledcotransformation because two genes from the donor bacterium have been transferred to the recipient via transformation. In this type of experiment, the…arrow_forwardGenetic transfer via transformation can also be used to map genes along the bacterial chromosome. In this approach, fragments of chromosomal DNA are isolated from one bacterial strain and used to transform another strain. The experimenter examines the transformed bacteria to see if they have incorporated two or more different genes. For example, the DNA may be isolated from a donor E. coli bacterium that has functional copies of the araB and leuD genes. Let’s call these genes araB+ and leuD+ to indicate the genes are functional. These two genes are required for arabinose metabolismand leucine synthesis, respectively. To map the distance betweenthese two genes via transformation, a recipient bacterium is used that is araB− and leuD−. Following transformation, the recipient bacterium may become araB+ and leuD+. This phenomenon is calledcotransformation because two genes from the donor bacterium have been transferred to the recipient via transformation. In this type of experiment, the…arrow_forwardGenetic transfer via transformation can also be used to map genes along the bacterial chromosome. In this approach, fragments of chromosomal DNA are isolated from one bacterial strain and used to transform another strain. The experimenter examines the transformed bacteria to see if they have incorporated two or more different genes. For example, the DNA may be isolated from a donor E. coli bacterium that has functional copies of the araB and leuD genes. Let’s call these genes araB+ and leuD+ to indicate the genes are functional. These two genes are required for arabinose metabolismand leucine synthesis, respectively. To map the distance betweenthese two genes via transformation, a recipient bacterium is used that is araB− and leuD−. Following transformation, the recipient bacterium may become araB+ and leuD+. This phenomenon is calledcotransformation because two genes from the donor bacterium have been transferred to the recipient via transformation. In this type of experiment, the…arrow_forward
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