Prescott's Microbiology
11th Edition
ISBN: 9781260211887
Author: WILLEY, Sandman, Wood
Publisher: McGraw Hill
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Chapter 16, Problem 5AL
Summary Introduction
Direct uptake of genetic materials (exogenous material) such as a plasmid or a linear DNA fragment by the cell through the cell membrane from the surroundings is called transformation. Transfer of genetic material from one bacterium to another bacterium is known as transformation. In transformation, the free DNA molecule is incorporated from the donor to the recipient cell or bacterium and that lead to a genetic change. In genetic transformation, if the bacterium is able to receive the DNA molecule and be transformed is known as competent. The DNA molecule can be bound to the bacterium and received inside. The capacity of receiving DNA is genetically determined.
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O O
In the experiment performed by Messelson and Stahl, bacterial cells grown in 15N-containing growth medium for many generations were then allowed to grow in 14N-containing medium.
Suppose they would have conducted the experiment the other way around: that is, to first grow bacterial cells in 14N-containing medium for many generations and then switching them to a
15N-containing growth medium. After two generations of growth in 15N media the results of a CsCl density gradient centrifugation of the DNA would be expected to show
O one band of intermediate density and one band of high-density (i.e. heavy band)
one band on intermediate density
O one band of intermediate-density and one band of low-density (i.e. light band)
one band of low density (L.e. light band) and one band of high density (1.e. heavy band).
one band of low density (I.e. light band), one band of intermediate density, and one band of high density (i.e. heavy band).
Which among A-D is false regarding growth and/or analysis of a bacterial culture?
A) O Fed-batch growth: typically results in an increased yield of cells over that obtained with batch growth
B) O Auxotroph: requires addition of specific growth factors in order to grow
C) O Prototroph: typically the wild type strain that possesses all the features indicative of the species
D) O None of A-D is false; all are true statements
Chapter 16 Solutions
Prescott's Microbiology
Ch. 16.1 - Retrieve, Infer, Apply List three ways in which...Ch. 16.1 - Compare and contrast the means by which the...Ch. 16.1 - Give examples of intragenic and extragenic...Ch. 16.1 - Retrieve, Infer, Apply Sometimes a point mutation...Ch. 16.1 - Retrieve, Infer, Apply Why might a missense...Ch. 16.2 - How would you screen for a tryptophan auxotroph?...Ch. 16.2 - Why is a small amount of histidine added to the...Ch. 16.2 - Retrieve, Infer, Apply Describe how replica...Ch. 16.2 - Retrieve, Infer, Apply Why are mutant selection...Ch. 16.2 - Retrieve, Infer, Apply Briefly discuss how...
Ch. 16.2 - Describe how you would isolate a mutant that...Ch. 16.2 - Prob. 5CCCh. 16.3 - How is mismatch repair similar to DNA polymerase...Ch. 16.3 - How is damaged DNA recognized by the UvrAB...Ch. 16.3 - Prob. 1CCCh. 16.3 - Retrieve, Infer, Apply What role does DNA...Ch. 16.3 - Retrieve, Infer, Apply When E. coli cells are...Ch. 16.3 - Explain how the following DNA alterations and...Ch. 16.4 - An antibiotic-resistance gene located on a...Ch. 16.4 - What four fates can DNA have after entering a...Ch. 16.4 - How does homologous recombination differ from...Ch. 16.5 - What features are common to all types of...Ch. 16.5 - How does a transposon differ from an insertion...Ch. 16.5 - What is simple (cut-and-paste) transposition? What...Ch. 16.5 - What effect would you expect the existence of...Ch. 16.6 - Prob. 1MICh. 16.6 - What is bacterial conjugation and how was it...Ch. 16.6 - For F+, Hfr, and F strains of E. coli, indicate...Ch. 16.6 - Describe how F+ F and Hfr conjugation processes...Ch. 16.6 - Compare and contract F+ F and F F conjugation.Ch. 16.7 - According to this model, what would happen if DNA...Ch. 16.7 - Prob. 1CCCh. 16.7 - Describe how transformation occurs in S....Ch. 16.7 - Discuss two ways in which artificial...Ch. 16.8 - Compare the number of transducing particles that...Ch. 16.8 - Why cant the gal and bio genes be transduced by...Ch. 16.8 - Describe generalized transduction and how it...Ch. 16.8 - What is specialized transduction and how does it...Ch. 16.8 - How might one tell whether horizontal gene...Ch. 16.8 - Why doesnt a cell lyse after successful...Ch. 16.8 - Describe how conjugation, transformation, and...Ch. 16.9 - As a replicative transposon, what would happen if...Ch. 16 - Prob. 1RCCh. 16 - Prob. 2RCCh. 16 - Prob. 3RCCh. 16 - Prob. 4RCCh. 16 - Prob. 5RCCh. 16 - Prob. 6RCCh. 16 - Mutations are often considered harmful. Give an...Ch. 16 - Mistakes made during transcription affect the cell...Ch. 16 - Suppose that transduction took place when a U-tube...Ch. 16 - Suppose that you carried out a U-tube experiment...Ch. 16 - Prob. 5ALCh. 16 - Prob. 6ALCh. 16 - Prob. 7AL
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Endosymbiotic Theory; Author: Amoeba Sisters;https://www.youtube.com/watch?v=FGnS-Xk0ZqU;License: Standard Youtube License