Prescott's Microbiology
11th Edition
ISBN: 9781260211887
Author: WILLEY, Sandman, Wood
Publisher: McGraw Hill
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Chapter 16.4, Problem 3CC
How does homologous recombination differ from site-specific recombination?
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With regard to the repair of double-strand breaks, what are the advantages and disadvantages of homologous recombination repair versus nonhomologous end joining?
With regard to the repair of double-strand breaks, what are the advantages and disadvantages of homologous recombination repair (HRR) versus nonhomologous end joining (NHEJ)?
What are site specific recombinases? Distinguish between serine and tyrosine recombinases.
Chapter 16 Solutions
Prescott's Microbiology
Ch. 16.1 - Retrieve, Infer, Apply List three ways in which...Ch. 16.1 - Compare and contrast the means by which the...Ch. 16.1 - Give examples of intragenic and extragenic...Ch. 16.1 - Retrieve, Infer, Apply Sometimes a point mutation...Ch. 16.1 - Retrieve, Infer, Apply Why might a missense...Ch. 16.2 - How would you screen for a tryptophan auxotroph?...Ch. 16.2 - Why is a small amount of histidine added to the...Ch. 16.2 - Retrieve, Infer, Apply Describe how replica...Ch. 16.2 - Retrieve, Infer, Apply Why are mutant selection...Ch. 16.2 - Retrieve, Infer, Apply Briefly discuss how...
Ch. 16.2 - Describe how you would isolate a mutant that...Ch. 16.2 - Prob. 5CCCh. 16.3 - How is mismatch repair similar to DNA polymerase...Ch. 16.3 - How is damaged DNA recognized by the UvrAB...Ch. 16.3 - Prob. 1CCCh. 16.3 - Retrieve, Infer, Apply What role does DNA...Ch. 16.3 - Retrieve, Infer, Apply When E. coli cells are...Ch. 16.3 - Explain how the following DNA alterations and...Ch. 16.4 - An antibiotic-resistance gene located on a...Ch. 16.4 - What four fates can DNA have after entering a...Ch. 16.4 - How does homologous recombination differ from...Ch. 16.5 - What features are common to all types of...Ch. 16.5 - How does a transposon differ from an insertion...Ch. 16.5 - What is simple (cut-and-paste) transposition? What...Ch. 16.5 - What effect would you expect the existence of...Ch. 16.6 - Prob. 1MICh. 16.6 - What is bacterial conjugation and how was it...Ch. 16.6 - For F+, Hfr, and F strains of E. coli, indicate...Ch. 16.6 - Describe how F+ F and Hfr conjugation processes...Ch. 16.6 - Compare and contract F+ F and F F conjugation.Ch. 16.7 - According to this model, what would happen if DNA...Ch. 16.7 - Prob. 1CCCh. 16.7 - Describe how transformation occurs in S....Ch. 16.7 - Discuss two ways in which artificial...Ch. 16.8 - Compare the number of transducing particles that...Ch. 16.8 - Why cant the gal and bio genes be transduced by...Ch. 16.8 - Describe generalized transduction and how it...Ch. 16.8 - What is specialized transduction and how does it...Ch. 16.8 - How might one tell whether horizontal gene...Ch. 16.8 - Why doesnt a cell lyse after successful...Ch. 16.8 - Describe how conjugation, transformation, and...Ch. 16.9 - As a replicative transposon, what would happen if...Ch. 16 - Prob. 1RCCh. 16 - Prob. 2RCCh. 16 - Prob. 3RCCh. 16 - Prob. 4RCCh. 16 - Prob. 5RCCh. 16 - Prob. 6RCCh. 16 - Mutations are often considered harmful. Give an...Ch. 16 - Mistakes made during transcription affect the cell...Ch. 16 - Suppose that transduction took place when a U-tube...Ch. 16 - Suppose that you carried out a U-tube experiment...Ch. 16 - Prob. 5ALCh. 16 - Prob. 6ALCh. 16 - Prob. 7AL
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- Compare and contrast conjugation, transduction, and transformation. Which processes, if any, involve homologous recombination and which, if any, do not?arrow_forwardWhich of the following is true of transposons? A) SINES are DNA-only transposons B) LINES are non-autonomous transposons Oa break induced repair is the mechanism by which this form of recombination C) occurs O D) recombinases form phosphotyrosine intermediates during transposition E) spo11 initiates the double-strand break to initiate transpostionarrow_forwardThe sequences of the recombination sites recognized by site-specific recombinases area) Partially asymmetricb) Partially symmetricc) Symmetricd) Palindromicarrow_forward
- describe Site-Specific Recombinationarrow_forwardEukaryotic cells can repair DNA through a process called homologous recombination. What type of DNA damage can be fixed through homologous recombination?arrow_forwardMake a list of the similarities and differences among homologous recombination, site-specific recombination, and transposition.arrow_forward
- Two pathways, homologous recombination and nonhomologous end joining (NHEJ), can repair double-strandbreaks in DNA. If homologous recombination is an errorfree pathway whereas NHEJ is not always error free, whyis NHEJ used most of the time in eukaryotes?arrow_forwardWhat types of matings are possible between F+, F−, Hfr, and F′ cells? What outcomes do these matings produce? What is the role of the F factor in conjugation?arrow_forwardWhich of the following is NOT true of transposition? A) a replicative form of transposition is possible for DNA-only transposition B) flanking direct repeats are a by-product of transposition formed at the transposition site C) inverted repeats are formed in all types of transposons D long-terminal repeats are found in the class of retrotransposons that resemble retrovirusesarrow_forward
- If homologous and site-specific recombination could not occur,what would be the harmful and the beneficial consequences?arrow_forwardWhat is a recombinant vector? How is a recombinant vector constructed? Explain how X-Gal is used in a method of identifying recombinant vectors that contain segments of chromosomal DNA.arrow_forwardConsider the following experiment. First, large populations of two mutant strains of Escherichia coli are mixed, each requiring a different, single amino acid. After plating them onto a minimal medium, 45 colonies grew. Which of the following may explain this result? A) The colonies may be due to back mutation (reversion). B) The colonies may be due to recombination. C) Either A or B is possible. D) Neither A nor B is possible.arrow_forward
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