Q: Describe the shotgun method for sequencing an entire genome.
A: Genome or genetic material of a cell is the DNA content present in the nucleus. DNA or…
Q: What are the five main steps in DNA cut-and-paste transposition?
A: The "cut and paste" method is used for saving conversion. The enzyme transposase acts as a pair of…
Q: What is the principle of next generation sequencing?
A: The process of determining the nucleic acid sequence is known as DNA sequencing.
Q: How can I develop a vector to express a bacterial gene (2,500 bp in size) in E. coli. How do I know…
A: Hi! As you have posted multiple questions, I will be answering the first two questions for you. If…
Q: What benefits are there when using a DNA microarray over a genetic marker such as a STR?
A: The term genetic refers to the DNA molecule present in the nucleus of cells. DNA or deoxyribonucleic…
Q: Describe the automated dideoxy chain termination method of DNA sequencing.
A: The chain termination DNA sequencing method is the Sangers sequencing in which the DNA fragment is…
Q: How does precision help in genetic engineering and recombinant technology? How does accuracy help in…
A: genetic engineering and recombinant technology are modern geneticist tools which help to edit genes…
Q: How does a DNA microarray work?
A: Gene microarray technology enables to deposit many different DNA sequences on a small glass slide,…
Q: What is the useful of the Development of highly sensitive and low-cost DNA agarose gel…
A: In today's medicine, extremely sensitive and low-cost DNA agarose gel electrophoresis is widely used…
Q: What are the considerations for choice of a vector in gene therapy?
A: HIV consists of single-stranded RNA in the replication form that has been transcribed into…
Q: Describe step 1: hybridization of template and primer in automated sanger sequencing
A: Introduction Sanger sequencing is also known as the “chain termination method”. It is a method for…
Q: What are the key differences between DNA microarrays and protein microarrays, and how they are used…
A: Introduction: A laboratory method used to recognize the expression of thousands of genes…
Q: What are selectable markers and why are they important to cloning vectors?
A: In recombinant DNA technology the vectors are the carrier for the target DNA sequence which needed…
Q: Outline four key steps involved in producing a recombinant DNA
A: Answer. Recombinant DNA technology (RDT) is also known as genetic engineering or cloning) is…
Q: What are transgenic Bacteria? Illustrate using any one example?
A: Genetic engineering is the modification of an organism’s genome for a beneficial outcome. Bacteria,…
Q: What are the vectors used in rDNA technology? briefly explain each
A: rDNA technology or Recombinant DNA technology is a widely used technology with the advancement of…
Q: How is recombinant DNA inserted into a genome using CRISPRediting?
A: The modern technology of genetics can be utilized to increase the genomes of various living…
Q: What is the difference between using genome build hg18 and hg19 in Genome-wide SNP arrays?
A: SNP (Single nucleotide protein) array is a type of DNA (deoxyribonucleic acid) microarray which is…
Q: Describe the benefits of using bacteria, yeast, mammalian and insect cells to make recombinant…
A: “Since you have asked multiple question, we will solve the first question for you. If youwant any…
Q: What is exome sequencing ? Why it is important ?
A: The exome has historically been described as the sequence in the genome that includes all exons of…
Q: What are sticky ends? State their significance in recombination DNA technology.
A: Recombinant DNA Technology is a method for creating artificial DNA by combining genetic resources…
Q: Describe 2 approaches that you can use to determine that you have successfully PCR amplified Sonic…
A: A gene is a genetic molecule that is passed down from one generation (parents) to the next…
Q: Explain the sequencing-by synthesis (SBS) approach ?
A: One of the well-known states that are known as fundamental topics in Arithmetic is sequence and…
Q: Explain why a selectable marker is frequently included in a cloning vector.
A: A cloning vector is a short bit of DNA that may be kept in an organism for a long time and into…
Q: What are the advantages of Next Generation Sequencing?
A: DNA sequencing is a biochemical method for determining the order of nucleotide bases, A, G, C, and…
Q: Why does one need a vector to clone a DNA
A: Cloning is the method of generating the number of copies of desired DNA I the host.
Q: Explain what is Flow cell DNA sequencing ?
A: What is DNA sequencing? It is the process of finding out the order of nucleotides i.e. adenine,…
Q: Give a step by step method and diagram of how reversible terminator sequencing works.
A: The reversible terminator sequencing or Illumina dye sequencing is a technique used to determine the…
Q: Discuss the principles , uses, advantages and disadvantages of illumina sequencing method
A: DNA copies obtained from PCR is subjected for gene sequencing through different gene sequencing…
Q: Discuss the advantages of next-generation sequencing technologies.
A: Next generation sequencing technique is also known as second generation sequencing where the DNA…
Q: What are the different types of PCR and their product concepts? Site and explain the comparison…
A: Introduction :- Polymerase Chain Reaction (PCR) is a molecular biology technique for making multiple…
Q: What are the major differences between dna probe and Primer
A: DNA as a genetic material in all the living organisms.
Q: What are the main differences between whole genome sequencing and whole exome sequencing?
A: whole genome sequencing is sequencing the entire genome of the organism, where as whole exome…
Q: How is genomic DNA prepared for shotgun sequencing?
A: Genomic DNA prepared for shotgun sequencing as follows : 1. ligating random genomic DNA fragments…
Q: What is DNA microarray used for?
A: It is a laboratory tool used to detect the expression of thousands of genes at the same time. The…
Q: How does the shotgun approach differ from the whole genome sequencing?
A: Biotechnology is concerned with exploitation of biological agents or their components for generating…
Q: What is Sanger sequencing? Why do we use ddNTP? How to read a DNA sequence gel? c. What is a cDNA…
A: Your question contains multiple sub-parts, and as per our policy, we only answer the first three…
Q: What is the difference between the first steps of DNA sequencing to PCR in terms of the number of…
A: PCR is expanded as a polymerase chain reaction. It is a technique that is used widely for the…
Q: What is the difference between a map-based approach to sequencing a whole genome and a whole-genome…
A: Deoxyribonucleic acid (DNA) is a double stranded helical genetic material containing thousands of…
Q: What is the major difference between the strategies of map based sequencing and shotgun sequencing?
A: DNA sequencing is a process in which sequence(order) of nucleotides in DNA is determined. It…
Q: Describe the different vectors that you would use for cloning DNA fragments of lengths 2,000;…
A: A cloning vector is a small piece of DNA that can be stably maintained in an organism, and into…
Q: Hi, I would like to know which program is used for the graphical presentation of the results of a…
A: A genome wide linkage association study (GWAS) is an approach used in genetic research to associate…
Q: Explain in detail types of DNA sequencing?
A: DNA sequencing is a technique which is used to determine the sequence of DNA nucleotides within the…
Q: How accurate is whole exome sequencing?
A: The goal of exome sequencing, also known as whole genome sequencing is to identify genetic variants,…
Q: Explain the advantages of DNA microarrays ?
A: A DNA microarray is a collection of microscopic DNA spots attached to a solid surface. Each DNA spot…
Q: What are five variables to consider when designing a primer for standard PCR and state why.
A: Polymerase chain reaction involves amplification of desired DNA strand using three steps i.e..…
Q: a) What are vectors? Describe extensively the roles vectors play in genetic engineering? Write short…
A: Note: Solving answer only for part A as per the guidelines, repost remaining part, please. The…
Q: Why is genome editing by CRISPR-Cas advantageous over traditionalmethods for creating knockout or…
A: Two crucial components make up the CRISPR-Cas9 system, which modifies DNA. These include the Cas9…
What is vector? Discuss 2 vectors that are commonly used in DNA recombinant technology.
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Solved in 2 steps
- How can site-specific recombination be used in recombinant DNA technology? Explain with an exampleWhat characteristics of plasmids makes them good cloning vectors? Discuss.List the advantages and disadvantages of using plasmids as cloning vectors. What advantages do BACs and YACs provide over plasmids as cloning vectors?
- Hi, I would like to know which program is used for the graphical presentation of the results of a meta-analysis of genome-wide linkage scans?What are the essential elements of a plasmid cloning vector? Discuss the importance of each element for dna cloning.Give a step by step method and diagram of how reversible terminator sequencing works.
- a) What are vectors? Describe extensively the roles vectors play in genetic engineering? Write short notees on the following: Recombinant DNA, Cloning b) What are restriction enzymes? Describe extensively the roles restriction enzymes play in genetic engineering? Write short notees on the following: Selectable markers, CloningWhat are the following features that are required facilitate cloning into a vector.In biotechnology, gene cloning is a very important technique. A vector is normally required to perform this process. The vector commonly used to transform a bacterial host cell is the plasmid. State the three (3) important regions of the plasmid. Elaborate your answer.