(a) Interpretation: The difference between the phosphofructokinase (PFK) activity in P. Furiosus and other animals should be determined. Concept introduction: Phosphofructokinase is an allosteric enzyme which converts fructose-1-phopshate to fructose-1,6-bisphosphate. It acts as a regulatory enzyme in the glycolysis process. High ATP concentration inside the cell inhibits the activity of PFK.

Question

Shown below is a substrate for a Trypsin. Draw the mechanism for this serine protease using the artificial substrate. Be sure to draw the catalytic triad, and show the role of the oxyanion hole. Draw the complete structure of every intermediate and product and PUSH ARROWS!!!!! Do not abbreviate structures using R and R' H₂N _N_CH. сно CH₂ CH₂ CH₂ NH d=19H₂ NH₂ O CH- H₂C HN O CH

Answer 1

Question

Chapter 16, Problem 57P

Interpretation Introduction

(a)

Interpretation:

The difference between the phosphofructokinase (PFK) activity in P. Furiosus and other animals should be determined.

Concept introduction:

Phosphofructokinase is an allosteric enzyme which converts fructose-1-phopshate to fructose-1,6-bisphosphate. It acts as a regulatory enzyme in the glycolysis process. High ATP concentration inside the cell inhibits the activity of PFK.

Interpretation Introduction

(b)

Interpretation:

Effect of ATP and AMP on ADP should be determined.

Concept introduction:

Phosphofructokinase is an allosteric enzyme which converts fructose-1-phopshate to fructose-1,6-bisphosphate. It acts as a regulatory enzyme in the glycolysis process. High ATP concentration inside the cell inhibits the activity of PFK.

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