Biochemistry
Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Chapter 16, Problem 45P
Interpretation Introduction

Interpretation:

Consequences of less sensitivity of fructose-1,6-bisphosphatase for fructose-2,6-bisphosphatase should be determined.

Concept introduction:

Glycolysis is the process that occurs in the cytoplasm of the cell. It is the breakdown of one molecule of glucose into two molecules of pyruvic acid. During this process, energy is released in the form of two moles of ATP. The purpose of glycolysis is to maintain the blood glucose level.

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disease. As such, a frontline treatment for Type 2 diabetes is the drug metformin, which acts indirectly to inhibit gluconeogenesis in the liver. You are a research biochemist who would like to develop new drugs that act to directly inhibit gluconeogenesis. You have just gained access to a library of thousands of small molecules of unknown activity, and you would like to identify lead compounds that have specific inhibitory activity against steps in the gluconeogenesis pathway. (a) into PEP in order to screen for inhibitors of enzymes specific to gluconeogenesis. Which enzymes do you need to purify, what cofactors and allosteric effectors do they require, and which reactants do you need to add to reconstitute the reactions for the first bypass? Which intermediates and products are generated? Your first approach is to reconstitute the initial set of bypass reactions that convert pyruvate (b) vitro reconstitution? What additional steps and enzymes are required in liver cells but are…
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