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You are growing E. coli in a laboratory in order to study their operons. The growth media you are using contains lactose, no glucose and no tryptophan. Using your knowledge of operons and their regulation, answer the following questions using the dropdown menus provided in Canvas.
- Which operons would be functional under these conditions?
- What repressors would be made? (NOTE: name the repressors using their gene names)
- Which repressor(s) would be made in the inactive form?
- Which repressor(s) would be made in the active form?
- Which repressor(s) under these conditions can bind the operator sequence?
- Which repressor(s)under these conditions cannot bind the operator sequence?
- What effect does lactose have on the bacterial cell’s lac operon?
- What does the absence of glucose do to the bacterial cell?
- You now add tryptophan to the cell. What would happen to the bacterial cell and its trp operon?
- What kind of regulation does lactose provide to an operon’s repressor?
Briefly answer these questions Once you have answered these questions
3. Explain your answer for question #G above (What effect does lactose have on the bacterial cell’s lac operon?)
4. Explain your answer for question #H above (What does the absence of glucose do to the bacterial cell?)
Explain your answer for #I above (You now add tryptophan to the cell. What would happen to the bacterial cell and its trp operon?)
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- The organization and function of the lac operon in E. coli is shown in the following figures: XXI Transcription Repressor mRNA Translation RNA polymerase M M Flask ZYA XXXX Active M-repressor protein Repressor active, operon off. The repressor protein binds with the operator, preventing transcription from the operon. (a) An inducible operon Copyright © 2007 Pearson Education, Inc., publishing as Benjamin Cummings Allolactose (inducer) Carbon/Energy Source 0 Z MInactive repressor protein (a) An inducible operon Transcription Translation XXX Permease B-Galactosidase Transacetylase Operon mRNA 3 Repressor inactive, operon on. When the inducer allolactose binds to the repressor protein, the inactivated repressor can no longer block transcription. The structural genes are transcribed, ultimately resulting in the production of the enzymes needed for lactose catabolism. Copyright © 2007 Pearson Education, Inc., publishing as Benjamin Cummings Suppose you inoculate three flasks of minimal…arrow_forwardYou are growing E. coli in a laboratory in order to study their operons. The growth media you are using contains lactose, no glucose and no tryptophan. Using your knowledge of operons and their regulation a.Which operons would be functional under these conditions? b.What repressors would be made? (NOTE: name the repressors using their gene names) c.Which repressor(s) would be made in the inactive form? d.Which repressor(s) would be made in the active form? e.Which repressor(s) under these conditions can bind the operator sequence? f.Which repressor(s)under these conditions cannot bind the operator sequence?arrow_forwardThere is a mutation in sequence of the lac operator that prevents the lac repressor from being able to recognize and bind to it. What would the status of transcription of the lac operon be under each of the following circumstances? Explain your answers. a-Lactose present, glucose present b-Lactose present, glucose absent c-Lactose absent, glucose present d-Lactose absent, glucose absentarrow_forward
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