Becker's World of the Cell (9th Edition)
Becker's World of the Cell (9th Edition)
9th Edition
ISBN: 9780321934925
Author: Jeff Hardin, Gregory Paul Bertoni
Publisher: PEARSON
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Chapter 16, Problem 16.9PS

Going Nuclear. Experiments have been performed with the nuclear localization sequence (NLS) of nucleoplasmin. In each case, a string of amino acids was added to pyruvate kinase, a glycolytic enzyme normally found in the cytosol. The sequence of each added string of amino acids is shown using single-letter amino acid designations. Consult the single-letter amino acid code table (Table 3-2, page 45) if you feel it is necessary. Many alanines in a row are denoted by (A)n, where n is the number of alanines. The following results were obtained:

Chapter 16, Problem 16.9PS, Going Nuclear. Experiments have been performed with the nuclear localization sequence (NLS) of

  1. (a) What sort of NLS does nucleoplasmin appear to have, based on these results? Explain your answer.
  2. (b) How does your conclusion about the NLS of nucleoplasmin compare to the NLS for SV40 large T antigen?
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Please help me with this question. More than one answer may be correct. THe graph relating to the information is included below. The figure shows the number of cells that have clusters of IRE1 molecules after those cells are treated with various levels of thapsigargin (Tg), a chemical that can induce ER stress. IRE1 can form these clusters when ER stress is induced and this clustering can cause activation of RNAse activity in IRE1. In this experiment, normal IRE1 was used (IRE1α) that can bind to Sec61, along with a modified version of IRE1 that binds to Sec61 more weakly than normal IRE1 (wIRE1α), and another modified version that binds to Sec61 more strongly than normal IRE1 (sIRE1α). From this figure you can conclude that: Question 18 options: IRE1 binding to Sec61 promotes the formation of IRE1 clumps IRE1 binding to Sec61 prevents the formation of IRE1 clumps co-translational translocation is a key process the golgi aparatus is heavily involved in the unfolded protein response The…
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Biomolecules - Protein - Amino acids; Author: Tutorials Point (India) Ltd.;https://www.youtube.com/watch?v=ySNVPDHJ0ek;License: Standard YouTube License, CC-BY