Becker's World of the Cell (9th Edition)
9th Edition
ISBN: 9780321934925
Author: Jeff Hardin, Gregory Paul Bertoni
Publisher: PEARSON
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Chapter 16, Problem 16.2CC
The GC content of the DNA from a newly discovered bacterium is 48%. What are the percentages of each of the four bases (A, T, G, C) in this organism?
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The DNA chromosome in E. coli contains approximately 4 million base pairs. The average gene contains about 1500 base pairs. Use this information to calculate the following (show all work ):
a) The length in meters of this chromosome.
b) The approximate number of genes in the chromosome (assuming no wasted DNA).
If the GC content of a DNA molecule is 60%, what are the molar percentages of the four bases (G, C, T, A)?
The Bacteria Escherichia coli DNA genome has a molecular mass of about 3.1 X 10° D. In your
answers, show how you came up to each result?
(a) How many base pairs does this bacterium contain?
(b) How many full double-helical turns does this DNA contain?
(c) How long is this DNA in µm?
Chapter 16 Solutions
Becker's World of the Cell (9th Edition)
Ch. 16 - Based on what you know about protein and DNA, why...Ch. 16 - The GC content of the DNA from a newly discovered...Ch. 16 - Prob. 1QCh. 16 - Changes in chromatin packing correlate with...Ch. 16 - You are studying a cytosolic protein, and your...Ch. 16 - Prior Knowledge. Virtually every experiment...Ch. 16 - DNA Base Composition. Based on your understanding...Ch. 16 - DNA Structure. Carefully inspect the...Ch. 16 - QUANTITATIVE DNA Melting. Figure 16-36 shows the...Ch. 16 - DNA Renaturation. You are given two samples of...
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- The Bacteria Escherichia coli DNA genome has a molecular mass of about 3.1 X 10 9 D. In your answers, show how you came up to each result?(a) How many base pairs does this bacterium contain? (b) How many full double-helical turns does this DNA contain? (c) How long is this DNA in micrometer?arrow_forwardWrite the complementary sequence for the following DNA sequence, in order from 3' to 5': 5′−CGATATTGAGCTAAGCTT−3′arrow_forwardThe chromosome of the bacterium (prokaryote) Escherichia coli has a size of approximately 4,000 kilobases (kb) [one kilobase = 1,000 bases]. What length of DNA (B form) would be contained in it? How does this length compare to the average size and/or volume of this bacterium?arrow_forward
- The oligonucleotide d-ATGCCTGACT was subjected to sequencing by Sanger’s dideoxy method, and the products were analyzed by electrophoresis on a polyacrylamide. Draw a diagram of the gel banding pattern obtained.arrow_forwardA primitive eukaryote was discovered that displayed a unique nucleic acid as its genetic material. Analysis provided the following information: (a) The general X-ray diffraction pattern is similar to that of DNA, but with somewhat different dimensions and more irregularity. (b) A major hyperchromic shift is evident upon heating and monitoring UV absorption at 260 nm. (c) Base-composition analysis reveals four bases in the following proportions: Adenine = 8% Guanine = 37% Xanthine = 37% Hypoxanthine = 18% (d) About 75 percent of the sugars are deoxyribose, while 25 percent are ribose. Postulate a model for the structure of this molecule that is consistent with the foregoing observations.arrow_forwardOne gram of cultured human cells contains about 109 cells and occupies roughly 1 mL. If the average molecular mass of a base pair is 660 daltons and each cell contains 6.4 x 109 bp, what mass of DNA is present in this one-gram sample? If all the DNA molecules in the sample were laid end to end to form a single thread, would it be long enough to reach from the Earth to the Moon (385,000 kilometers)?arrow_forward
- What is the axial ratio (length:diameter) of a DNA molecule 20 μm long?arrow_forwarda) Explain the effect of the guanine:cytosine ratio on melting temperature of DNA. b) The Hershey-Chase experiment proved that DNA is the genetic material and not protein. Explain in detatil how this experiment was conducted.arrow_forwardIf a double-stranded DNA molecule is 22% G, what is the percentage of A, T, and C? Explain.arrow_forward
- A circular, double-stranded DNA contains 2100 base pairs. The solution conditions are such that DNA has 10.5 bp/turn. (a) What is Lo for this DNA? (b) The DNA is found to have 12 left-handed superhelical turns. What is the superhelix density o?arrow_forwardWhat is the concentration of a DNA solution that absorbs 0.812 and 0.463 at 260 and 280 nm, respectively? Is the DNA solution considered to be good quality? Why or why not?arrow_forwardGiven the sequence shown below, write the complementary DNA sequence, using the base-pairing rules, as well as the directionality of the strands: 5'- CGAGGCTAGGTTAACCTG-3'arrow_forward
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