Becker's World of the Cell (9th Edition)
9th Edition
ISBN: 9780321934925
Author: Jeff Hardin, Gregory Paul Bertoni
Publisher: PEARSON
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Chapter 16, Problem 16.4CC
You are studying a cytosolic protein, and your friend is studying nuclear localization sequences in her favorite protein. How could you work together to show that her NLS is sufficient to cause any protein, including yours, to move into the nucleus?
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Consider Molecule X, which is found in all living cells. Each form of this molecule is keyed to the genetic code for a particular amino acid. When activated, the
amino acid is covalently bonded to one end of this molecule, and it carries the amino acid to a ribosome to take part in protein assembly.
What is the name of X? Your answer should be one word, or a short two- or three-word phrase. Spelling counts.
Note: if there is more than one possible answer, separate each answer with a comma.
0
The nucleus is the largest of the eukaryotic organelles and contains the genome of the organism.
a) describe the proteins and process of nuclear import and export.
b) describe, in general terms, an experiment that could be conducted to show that there are specific signals for nuclear import or export.
Consider two different proteins that are leaving from the Golgi in order to arrive in their common destination, a lysosome. In order for these two different proteins to be sent to the same place, what should they have in common?
A) They must have the same primary structure
B) They must have been imported into the cell by endocytosis.
C) They must have the same tertiary structure
D) They probably have the same glycosylation pattern.
..
Chapter 16 Solutions
Becker's World of the Cell (9th Edition)
Ch. 16 - Based on what you know about protein and DNA, why...Ch. 16 - The GC content of the DNA from a newly discovered...Ch. 16 - Prob. 1QCh. 16 - Changes in chromatin packing correlate with...Ch. 16 - You are studying a cytosolic protein, and your...Ch. 16 - Prior Knowledge. Virtually every experiment...Ch. 16 - DNA Base Composition. Based on your understanding...Ch. 16 - DNA Structure. Carefully inspect the...Ch. 16 - QUANTITATIVE DNA Melting. Figure 16-36 shows the...Ch. 16 - DNA Renaturation. You are given two samples of...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- You are studying a eukaryotic protein PKK in the lab. You fuse the DNA encoding GFP (green fluorescent protein) to the C terminus of the PKK gene. You also create the following GFP-PKK variants -GFP Wild- type: N- Signal sequence Transmembrane domain Kinase domain Variant 1: N- GFP Transmembrane domain Kinase domain Variant 2: N- -GFP Signal sequence Transmembrane domain Variant 3: N- -GFP Signal sequence Kinase domain Variant 4: N- -GFP Kinase domain If you examine wild-type PKK trafficking in a eukaryotic cell, which of the following are true? Select all that apply This fusion protein will be trafficked to the nucleus This fusion protein is a cell membrane protein This fusion protein will be trafficked to the Endoplasmic Reticulum This fusion protein is a nuclear proteinarrow_forwardYou are studying a eukaryotic protein PKK in the lab. You fuse the DNA encoding GFP (green fluorescent protein) to the C terminus of the PKK gene. You also create the following GFP-PKK variants Wild- type: N- -GFP Signal sequence Transmembrane domain Kinase domain Variant 1: N- -GFP Transmembrane domain Kinase domain Variant 2: N- GFP Signal sequence Transmembrane domain Variant 3: N- GFP Signal sequence Kinase domain Variant 4: -GFP Kinase domain If you examine PKK variant 4 trafficking in a eukaryotic cell, which of the following are true? Select all that apply This fusion protein will be made as a cytosolic protein This fusion protein will not function like the wild-type protein This fusion protein will traffic to the cell membrane This fusion protein will not traffic to the cell membranearrow_forwardThe nucleus a) has a single membrane and compartmentalizes the genetic material b) has a double membrane joined by nuclear pores, is continuous with the endoplasmic reticulum membrane, and compartmentalizes the nuclear genetic material c) has a double membrane joined by nuclear pores, is continuous with the smooth endoplasmic reticulum membrane only, and compartmentalizes the nuclear genetic material d) None of these are accuratearrow_forward
- The nucleus houses and protects the DNA within eukaryotic cells. To keep the DNA safe, the passage of materials in and out of the nucleus is highly restricted. Explain the route by which molecules can enter/exit the nucleus. In addition, briefly describe 5 types of proteins that are allowed entry into the nucleus and their roles.arrow_forwardThe biological process shown in Figure 1 occurs in a eukaryotic cell’s nucleus. d) Name X and state how it functions in a protein synthesis. e) Give three differences between molecules X and Y.arrow_forwardUnlike bacterial cells, the nucleus of a eukaryotic cell is bounded by a double-layered membrane studded by complex pores. How do you think this might affect traffic between the DNA and cytoplasm of a eukaryotic cell compared to that of a prokaryotic cell?arrow_forward
- List three potential organelle destinations for a free ribosome (cytosolic-produced) protein via transmembrane protein translocation:arrow_forwardExplain how and why chromatin is condensed in the nucleus?arrow_forwardWhat is the structure and components of the nucleosome? Briefly explain with your words.arrow_forward
- In eukaryotic cells, secreted proteins are initially directed to the endoplasmic reticulum and then via the Golgi, where they are released into the extracellular environment through secretory vesicles. A more easier way would be for secretory protein-producing ribosomes to be localised to a translocon in the plasma membrane, with the protein being secreted directly during translation. Consider three possible benefits of the more roundabout method for protein secretion versus the simpler, more straightforward approach indicated.arrow_forwardWhy is it an advantage for eukaryotic cells to have different compartments (aka organelles) in the cell (separated by membranes) with different environments (ex: different pH’s, different enzymes present, etc)?arrow_forwardEach type of molecule listed below is recognized by a specific class of nuclear import receptor (importin) or nuclear export receptor (exportin). For each type of molecule, state whether you think it is recognized by an importin or exportin and explain why (in terms of where that molecule is synthesized, what that molecule does, and where it does it). (a) RNA polyermase II: (b) ribosomal protein: (iii) †RNA:arrow_forward
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