Becker's World of the Cell (9th Edition)
9th Edition
ISBN: 9780321934925
Author: Jeff Hardin, Gregory Paul Bertoni
Publisher: PEARSON
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Chapter 16, Problem 16.4PS
QUANTITATIVE DNA Melting. Figure 16-36 shows the melting curves for two DNA samples that were thermally denatured under the same conditions.
Figure 16-36 Thermal Denaturation of Two DNA Samples. See Problem 16-4.
- (a) What conclusion can you draw concerning the base compositions of the two samples? Explain.
- (b) How might you explain the steeper slope of the melting curve for sample A?
- (c) Formamide and urea are agents known to form hydrogen bonds with pyrimidines and purines. What effect, if any, would the inclusion of a small amount of formamide or urea in the incubation mixture have on the melting curves?
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May you please help me with this?
A sample of purified DNA was incubated with deoxyribonuclease (DNAse) at 37oC. An
aliquot was removed from the reaction mixture every minute for 5 minutes and
the A260 recorded. The following data were obtained.
Time (min) A260
0 0.60
1 0.64
2 0.67
3 0.70
4 0.72
5 0.73
Describe the action of deoxyribonuclease on DNA and explain the increase in
A260.
True or False.
Do single-stranded DNA strands stabilize other strands?
Chapter 16 Solutions
Becker's World of the Cell (9th Edition)
Ch. 16 - Based on what you know about protein and DNA, why...Ch. 16 - The GC content of the DNA from a newly discovered...Ch. 16 - Prob. 1QCh. 16 - Changes in chromatin packing correlate with...Ch. 16 - You are studying a cytosolic protein, and your...Ch. 16 - Prior Knowledge. Virtually every experiment...Ch. 16 - DNA Base Composition. Based on your understanding...Ch. 16 - DNA Structure. Carefully inspect the...Ch. 16 - QUANTITATIVE DNA Melting. Figure 16-36 shows the...Ch. 16 - DNA Renaturation. You are given two samples of...
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- The Qiagen DNeasy DNA extraction kit is used to extract DNA from a liver cell line for subsequent molecular experiments. (i) (ii) for checking DNA purity using a spectrophotometer. Explain the principle behind this kit in DNA extraction. After the DNA is obtained, the purity of DNA should be determined. Discuss the stepsarrow_forwardRead it carefully.. Draw only correct diagrams.. In the Watson-Crick DNA base pairing model, Adenine (A) binds to thymine (T), guanine (G) binds to cytosine (C). 1. Draw the structures of thymine and adenine stabilized by Watson-Crick base pair interaction. 2. Also draw the structure of the amide group of glutamine in an interaction of this T-A pair in a way that maximally satisfies the hydrogen bonding capacity of amide.arrow_forwardPlease help me solve this problem. I am really having a hard time understanding this lesson. Please help. Kindly provide all the necessary information to this problem. Thank you! Please answer numbers 1-5 determine what amino acid will be formed from the given DNA strand below: 3’ T A C A T G C C G A A T G C C 5’ Note: Prepare the partner strand of this DNA. Discuss how will replication happen by mentioning the enzyme needed then transcribe to form mRNA. Discuss what will happen to mRNA, then translate, mentioning the anticodon to be used. Look at the genetic code to know what amino acid will become part of the polypeptide chain. 1. Partner DNA strand 2. the mRNA strand 3. The tRNA 4. the formed amino acids 5. the discussion of the entire procedurearrow_forward
- a.) Explain briefly how silica allows us to purify DNA. Be sure to state the relevant biochemical features of silica and DNA as well as any relevant reagents or steps that are important for the purification. Your answer should explain both how we get the DNA to bind in the first place, and how we are able to remove the DNA. You do not (and should not) explain earlier steps in a DNA purification; focus you answer on the silica:DNA interaction. b.) In theory, can a silica spin column also be used to purify RNA? If so, explain how (and why we did not observe RNA when analyzing plasmid DNA via agarose gel electrophoresis). If not, explain why not.arrow_forwardClose contact. Examination of the structure of DNA polymerases bound to nucleotide analogs reveals that conserved residues come within van der Waals contact of C-2'C-2' of the bound nucleotide. What is the potential significance of this interaction?arrow_forwardno explanation necessary! Letter of answer only. Part I: Multiple choice 1. The DNA base pairing rules are: a) Any combination of the bases. b) T pairs with C, and A pairs with G. c) A pairs with T, and C pairs with G. d) C pairs with A, and T pairs with G. 2. DNA in cells is damaged: a) Millions of times a day. b) By collisions with other molecules or by chemical accidents and radiation. c) Not very often¸ and by radiation only. d) a and b 3. For genes that code for proteins, which molecule conveys the information from the gene to the ribosome? a) DNA b) mRNA c) tRNA d) rRNA 4. Which of the molecules below is produced during replication? a) mRNA b) rRNA c) tRNA d) DNA 5. Why is there a difference between the synthesis of a lead strand and that of a discontinuous strand in DNA molecules? a) The origins of replication are found only at end 5' of the molecule. b) Helicase and protein factors act at the extremity of 5'. c) DNA polymerases can only add new nucleotides at the extremity 3' a…arrow_forward
- DNA QUANTITATION If you have the extracted/purified DNA and will not be immediately loaded in the spectrophotomer, how can you maximize the storage life of DNA? What practices will maximize the quality of your DNA sample?arrow_forwardShow all work. 1. A) Produce a double-stranded piece of DNA that is 11 base pairs long using the letters A, C, G, T and label the ends of both strands using 3' and 5' appropriately. B) Calculate the ratio of (A+T)/(G+C) and (A+G)/(C+T). Explain why one ratio will always be equal to 1.0?arrow_forwardTrue or False. Each time the genome is replicated, half the newly synthesized DNA is stitched together from Okazaki fragments. Explain your answer in 1-2 sentences.arrow_forward
- TRUE OR FALSE. Studies have confirmed that damaged to both the double strands can be reversed via single stranded annealing.arrow_forwardTRUE OR FALSE 1. Nitrogenous bases cannot undergo electrophilic aromatic substitution.2. Cytosine forms three hydrogen bonds with guanine while Adenine forms two hydrogen bonds with Thymine in themessenger RNA.3. The glycosidic bond in nucleotides is stable in dilute ammonium hydroxide solution and dilute Sodium bicarbonatesolution.4. In gel electrophoresis, a DNA is expected to migrate towards the cathode.5. Tautomerism is a phenomenon observed in both purine and pyrimidine bases.arrow_forwardInstructions In this image, the DNA fragments traveled from top to bottom. Use the drawpad to indicate the positive (+) and negative (-) ends of the gel. Remember, DNA is negatively- charged. Use two colors to circle the shortest fragment and the longest fragment in each of the five samples. 1 2 3 4 5 ||arrow_forward
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