Becker's World of the Cell (9th Edition)
9th Edition
ISBN: 9780321934925
Author: Jeff Hardin, Gregory Paul Bertoni
Publisher: PEARSON
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Chapter 16, Problem 16.2PS
DNA Base Composition. Based on your understanding of the rules of complementary base pairing, answer the following questions:
(a) You analyze a DNA sample and find that its base composition is 30% A, 20% T, 30% G, and 20% C. What can you conclude about the structure of this DNA?
(b) For a double-stranded DNA molecule in which 40% of the bases are either G or C, what can you conclude about its content of the base A?
(c) For a double-stranded DNA molecule in which 40% of the bases are either G or T, what can you conclude about its content of the base A?
(d) For a double-stranded DNA molecule in which 15% of the bases are A, what can you conclude about its content of the base C? Would you expect the Tm of this DNA to be above or below 90°C? (Hint: Refer to Figure 16-12.)
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DNA Structure
A. Draw an A-T base pair with the appropriate number of hydrogen bonds. You don’t have to include all the details such as every side-group but do depict the 3’ OH groups.
B. What is meant by anti-parallel when referring to a DNA molecule?
C. What are the major and minor grooves in the DNA structure and what significance do they have?
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composition of DNA from many different species.
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N.
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Chargaff noted that the molar quantity of A_was
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T. and the molar quantity of C was always
approximately equal to the molar quantity of G. How
were Chargaff's results explained by the
structural model of DNA proposed by Watson
and Crick?
N
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N.
O= P-OCH,
OH
OH
Using Fig. as a guide, draw the complete structure of a nucleoside triphosphate before and after it becomes incorporated into a polynucleotide chain. Draw the structure that would result if the newly formed phosphodiester bond were hydrolyzed.
Chapter 16 Solutions
Becker's World of the Cell (9th Edition)
Ch. 16 - Based on what you know about protein and DNA, why...Ch. 16 - The GC content of the DNA from a newly discovered...Ch. 16 - Prob. 1QCh. 16 - Changes in chromatin packing correlate with...Ch. 16 - You are studying a cytosolic protein, and your...Ch. 16 - Prior Knowledge. Virtually every experiment...Ch. 16 - DNA Base Composition. Based on your understanding...Ch. 16 - DNA Structure. Carefully inspect the...Ch. 16 - QUANTITATIVE DNA Melting. Figure 16-36 shows the...Ch. 16 - DNA Renaturation. You are given two samples of...
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- Close contact. Examination of the structure of DNA polymerases bound to nucleotide analogs reveals that conserved residues come within van der Waals contact of C-2'C-2' of the bound nucleotide. What is the potential significance of this interaction?arrow_forwardTrue or False. In a comparison between the DNAs of related organisms such as humans and mice, conserved sequences represent functionally important exons and regulatory regions, and non-conserved sequences generally represent noncoding DNA. Explain your answer in 2-3 sentences.arrow_forwardRead it carefully.. Draw only correct diagrams.. In the Watson-Crick DNA base pairing model, Adenine (A) binds to thymine (T), guanine (G) binds to cytosine (C). 1. Draw the structures of thymine and adenine stabilized by Watson-Crick base pair interaction. 2. Also draw the structure of the amide group of glutamine in an interaction of this T-A pair in a way that maximally satisfies the hydrogen bonding capacity of amide.arrow_forward
- True or False. Do single-stranded DNA strands stabilize other strands?arrow_forwardCompare and contrast the structure of DNA and RNA. Be sure to describe each of the three components of a nucleotide for both DNA and RNA along with the types of bonds formed between the components. In addition, explain: how the nucleotides link together to form each molecule, why the prime ends are labeled 5’ and 3’, what antiparallel is, what phospodiester linkages are and what complementary base pairing is.arrow_forwardDNA Stability/Chapter 4 A solution of DNA contains two different DNA molecules. Molecule 1 is 500 base pairs and contains 100 A’s. Molecule 2 is 500 base pairs and contains 300 A’s. (1) How many AT base pairs and GC base pairs is in each DNA molecule? (2) How many, G’s, A’s, T’s and C’s are in each molecule? (3) Draw one figure that shows the relative melting temperature of the two DNA molecules.arrow_forward
- . Some naturally occurring polynucleotide sequences are palindromic; that is, they are self-complementary about an axis of symmetry. Such a sequence is TCAAGTCCATGGACTTGG AGTTCAGGTACCTGAACC Show how this structure might form a double hairpin, or cruciform, conformation. Indicate the center of symmetry in the sequence and the bounds of the cruciform.arrow_forwardPlease help me solve this problem. I am really having a hard time understanding this lesson. Please help. Kindly provide all the necessary information to this problem. Thank you! Please answer numbers 1-5 determine what amino acid will be formed from the given DNA strand below: 3’ T A C A T G C C G A A T G C C 5’ Note: Prepare the partner strand of this DNA. Discuss how will replication happen by mentioning the enzyme needed then transcribe to form mRNA. Discuss what will happen to mRNA, then translate, mentioning the anticodon to be used. Look at the genetic code to know what amino acid will become part of the polypeptide chain. 1. Partner DNA strand 2. the mRNA strand 3. The tRNA 4. the formed amino acids 5. the discussion of the entire procedurearrow_forwardBiochemist Erwin Chargaff was the first to note that, in DNA, [A] = [T] and [G]= [C], equalities now called Chargaff’s rule. Using this rule, determine the percentages of all the bases in DNA that is 20% thymine.arrow_forward
- The Basic Structure of DNA 1. Nucleotides are the monomer subunits of DNA polymers. Review the structure of a nucleotide by labeling the three main parts: the sugar, the phosphate group and the nitrogenous base of the one shown below. NH2 N- || ОН 2. Which part (sugar, phosphate, or nitrogenous base) of the four types of nucleotides differ? 3. Based on the complementary base pairing rules we know that: A(denosine) pairs with and that G(uanine) pairs with 4. What two types of bonds are found in a DNA molecule and where are they found? а. bonds are found b. bonds are found O=a-oarrow_forwardTRUE OR FALSE] 11. The structure of the DNA, being super coiled, promotes increased viscosity of the molecule.12. The stacking position of the nitrogenous bases is caused by intramolecular Van der Waals Interaction.13. The histone complex is a quaternary protein structure.14. The difference between ribose and deoxyribose is in the presence of oxygen in carbon number 3 of the sugar moiety.15. The sedimentation coefficient of the nucleic acid is higher compared to ribosomes.arrow_forward. A viral DNA is analyzed and found to have the following base com- position, in mole percent: A = 32, G = 16, T = 40, C = 12. (a) What can you conclude about the structure of this DNA? (b) What kind of secondary structure do you think it would have?arrow_forward
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