Life: The Science of Biology
11th Edition
ISBN: 9781319010164
Author: David E. Sadava, David M. Hillis, H. Craig Heller, Sally D. Hacker
Publisher: W. H. Freeman
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Chapter 14.1, Problem 2R
Summary Introduction
To review:
The setup of experiments on the mutant strains of Neurospora to determine the order of biochemical pathway.
Introduction:
Neurospora is a red-bread mold belonging to the phylum Ascomycota. It has ability to manufacture its own amino acids in a minimal media. The reason for its use in the laboratory experiments is due to its easy and economical multiplication with least requirements. It has a haplontic life cycle with a short diploid stage and produces multiple progenies at once.
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A series of auxotrophic mutants were isolated in Neurospora. Examination
of fungi containing these mutations revealed that they grew on minimal
medium to which various compounds (A, B, C, D) were added; growth
responses to each of the four compounds are presented in the following
table. Give the order of compounds A, B, C, and D in a biochemical
pathway. Outline a biochemical pathway that includes these four
compounds and indicate which step in the pathway is affected by each of
the mutations.
Compound
Mutation number
А в С
A
D
134
276
987
773
772
146
333
123
+ + I +
Yeast cells are eukaryotes, and they require a functional secretory pathway to grow
and to maintain cellular organization. But surprisingly, when SRP is removed from
yeast cells by deleting the relevant genes, the cells are still alive (although they grow
slowly).
a) How can yeast survive without SRP? Propose two alternative hypotheses.
b) How might electron microscopy of normal and SRP-deficient yeast cells help you
to distinguish between these two hypotheses?
Describe Hershey-Chase experiment .
Chapter 14 Solutions
Life: The Science of Biology
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