Biology
5th Edition
ISBN: 9781260487947
Author: BROOKER
Publisher: MCG
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Chapter 13.3, Problem 3EQ
Summary Introduction
To determine: The material that was the most effective at causing the degradation of mex-3 mRNA.
Introduction: For silencing mRNAs, often antisense RNAs are used. Fire and Mello conducted an experiment using the mex-3 gene to prove that double-stranded RNAs formed by combining sense and antisense strands is very effective in gene silencing.
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E30. An electrophoretic mobility shift assay can be used to study the
binding of proteins to a segment of DNA. In the experiment shown
here, an EMSA was used to examine the requirements for the bind-
ing of RNA polymerase II (from eukaryotic cells) to the promoter
of a protein-encoding gene. The assembly of general transcription
factors and RNA polymerase II at the core promoter is described
in Chapter 12 (Figure 12.14). In this experiment, the segment of
DNA containing a promoter sequence was 1100 bp in length. The
fragment was mixed with various combinations of proteins and
then subjected to an EMSA.
Lane 1: No proteins added
Lane 2: TFID
Lane 3: TFIIB
Lane 4: RNA polymerase I|
Lane 5: TFID + TFIIB
Lane 6: TFID + RNA
1 2
4 5 6
polymerase II|
Lane 7: TFIID +
TFIIB + RNA
polymerase I|
1100 bp
Explain which proteins (TFIID, TFIIB, or RNA polymerase II) are
able to bind to this DNA fragment by themselves. Which transcrip-
tion factors (i.e., TFIID or TFIIB) are needed for the binding of…
Give typing answer with explanation and conclusion
a) List three eukaryotic gene expression mechanisms that do not occur in prokaryotes. For two of these, give specific examples and the functional outcomes.
b) Describe what is meant by the term “RNA silencing”.
c) Using diagrams, give two examples of RNA silencing mechanisms and indicate one difference.
WHAT IF? What would be the effect of treating cellswith an agent that removed the cap from mRNAs?
Chapter 13 Solutions
Biology
Ch. 13.1 - Prob. 1CCCh. 13.2 - Prob. 1CCCh. 13.3 - Prob. 1EQCh. 13.3 - Prob. 2EQCh. 13.3 - Prob. 3EQCh. 13.3 - Effects of Non-coding RNAs on Translation and mRNA...Ch. 13.4 - Non-coding RNAs and Protein Sorting Core Skill:...Ch. 13.5 - Core Skill: Modeling The goal of this modeling...Ch. 13 - Prob. 1TYCh. 13 - Prob. 2TY
Ch. 13 - Prob. 3TYCh. 13 - Prob. 4TYCh. 13 - Prob. 5TYCh. 13 - Prob. 6TYCh. 13 - With regard to miRNAs and siRNAs, which of the...Ch. 13 - Cas1 and Cas2 proteins play a role during which of...Ch. 13 - Which of the following components bind to...Ch. 13 - Abnormalities in the expression of ncRNAs are...Ch. 13 - An ncRNA may have one or more of the following...Ch. 13 - What is RNA interference (RNAi)? Explain how the...Ch. 13 - Prob. 3CQCh. 13 - Prob. 1COQCh. 13 - Go to the PubMed website and search for non-coding...
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- 8:20 ■■ LTE < Spring 2024 - Senior Comprehensives (... Question 6ɔ (ividitudtory) In the Lac operon, what happens when allolactose attaches to the repressor protein? a) b) c) the repressor is induced to attach to the promoter RNA polymerase is induced to attach to the operator lactose synthesis enzymes are produced by genes Z, Y,A d) the repressor is inactivated Question 88 (Mandatory) What is the purpose of X-chromosome inactivation in mammals? a) It accomplishes dosage compensation. b) It assists in the interactions beteeen genes Tra and Dsx. c) It prevents trisomy from occurring. d) It produces the testis determination factor. Question 94 (Mandatory) Duchenne muscular dystrophy is inherited in an X- linked recessive pattern. A female is known to be heterozygous for Du muscular dystrophy XD xdarrow_forwardHow have microarrays demonstrated that, although allcells of an organism have the same genome, some genes areexpressed in almost all cells, whereas other genes show cellandtissue-specific expression?arrow_forwardExploring Further: The table below outlines the stpes in eukaryotic gene expressions. Briefly summarize each steps given. Gene Expression Steps Molecules Involved Summary What molecules and proteins are What happens during this involved in this steps? step? Transcription RNA Splicing MRNA Transport Translation Protein Processingarrow_forward
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