Biology
5th Edition
ISBN: 9781260487947
Author: BROOKER
Publisher: MCG
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Textbook Question
Chapter 13, Problem 9TY
Which of the following components bind to tracrRNA?
- a. crRNA and Cas1 protein
- b. crRNA and Cas2 protein
- c. crRNA and Cas9 protein
- d. crRNA only
- e. Cast and Cas2 proteins
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The main target for Cas9 to cleave is:
O a. Spacer DNA sequence
O b. TracrRNA sequence
C.
CRRNA loop
d. CRRNA sequence
PAM sequence
e.
CLEAR MY CHOICE
Which one of the following applies to the DutUng E. Coli cells
a. N-glycosylase is intact.
b. dATPase is not produced.
С.
The dUTPase is disrupted.
O d. They express both dUTPase and N-glycosylase.
О е.
dTTPase is inactive.
CLEAR MY CHOICE
When an ncRNA functions as a decoy, ita. contains binding sites for many different proteins, therebypromoting the formation of a large complex.b. recognizes other ncRNAs and sequesters them, therebypreventing them from working.c. can alter its conformation, allowing it to switch betweenactive and inactivate conformations.d. may physically prevent or block a cellular process fromhappening.
Match each factor involved in miRNA biogenesis with its function
pri-miRNA
forms a hairpin that is recognized by Drosha and DGCR8
A.
Drosha and DGCR8
cleave non-complementary tails off pri-miRNAS
B.
exportin 5
C.
transfers pre-miRNA from the nucleus to the cytoplasm
Dicer
D. processes pre-miRNAS into mature 20-25 nt miRNAS
binds to mRNAs and represses their translation or targets them for degradation
Е.
RISC
Chapter 13 Solutions
Biology
Ch. 13.1 - Prob. 1CCCh. 13.2 - Prob. 1CCCh. 13.3 - Prob. 1EQCh. 13.3 - Prob. 2EQCh. 13.3 - Prob. 3EQCh. 13.3 - Effects of Non-coding RNAs on Translation and mRNA...Ch. 13.4 - Non-coding RNAs and Protein Sorting Core Skill:...Ch. 13.5 - Core Skill: Modeling The goal of this modeling...Ch. 13 - Prob. 1TYCh. 13 - Prob. 2TY
Ch. 13 - Prob. 3TYCh. 13 - Prob. 4TYCh. 13 - Prob. 5TYCh. 13 - Prob. 6TYCh. 13 - With regard to miRNAs and siRNAs, which of the...Ch. 13 - Cas1 and Cas2 proteins play a role during which of...Ch. 13 - Which of the following components bind to...Ch. 13 - Abnormalities in the expression of ncRNAs are...Ch. 13 - An ncRNA may have one or more of the following...Ch. 13 - What is RNA interference (RNAi)? Explain how the...Ch. 13 - Prob. 3CQCh. 13 - Prob. 1COQCh. 13 - Go to the PubMed website and search for non-coding...
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- Complete the following sentence: Transcription of DNA by RNA polymerase II synthesises microRNA precursor transcript in: A. either the 3′ to 5′ direction or the 5′ to 3′ direction depending on the orientation of the gene B. a discontinuous fashion. C. the 3′ to 5′ direction. D. both the 3′ to 5′ direction and the 5′ to 3′ direction simultaneously E. the 5′ to 3′ direction.arrow_forwardProvide the SIGNIFICANT differences between the following: 1. Regulation AFTER transcription mRNA processing miRNAs a. b. 2. Types of Genes Facultative Constitutive a. b.arrow_forwardAn ncRNA may have the following functions: scaffold, guide, alterer of protein function or stability, ribozyme, blocker, and/or decoy. Which of these functions are mediated by each type of ncRNA listed next? Note: A singlencRNA may have more than one function. A. tRNAB. rRNAC. SRP RNAD. piRNAarrow_forward
- Choose the item in column 2 that best matches each item in column 1. A. Provides information for production of protein B. Binds to promoter C. Promoter has a box A and box B consensus sequences D. Autocatalytic RNA molecules E. Associated with transcription termination F. Polymerization of ribonucleotides to form RNA molecules G. 7-methyl guanosine H. MRNA prior to processing select - 1. Core RNA polymerase select 2. rho (p) factor select 3. hnRNA select - 4. RNA select -5. RNA polymerase holoenzyme select 6. Ribozymes select - 7. 5' MRNA cap select 8. MRNAarrow_forwardDefine the following terms:a. cistronb. operonc. miRNAd. siRNAe. snoRNAarrow_forwardWhich of the following is an intron and intergenic coded long transcripts, processed into stem and loop structures that may bind specifically or otherwise? a. spliceosome RNA b. LncRNA c. miRNA d. piRNA e. siRNAarrow_forward
- Describe the following a. Core enzyme RNA polymerase b. Sigma factor c. Promoter consensus sequence d. Rho Dependent Factor e. Intrinsic Factor f. Abortive Initiationarrow_forwardmicroRNAs (miRNAs) play important roles in gene regulation. What is the correct order of steps (using letters A - E) for miRNA biogenesis or formation? A. Synthesis of pri-miRNA B. Targeting of mRNA for translation repression or mRNA degradation C. Loading into RISC complex D. Formation of mature miRNA E. Formation of pre-miRNA O AECDB O CDAEB O ACBDE O ADCEB O ECABDarrow_forwardmiRNAS P. are synthesized as long pri-miRNAs (up to 1000 nucleotides). Q. are transcribed by RNA polymerase II. R. are synthesized from pre-miRNAs by an RNase III enzyme Drosha in the cytoplasm. S. are synthesized from pri-miRNAs by an enzyme dicer in the nucleus. a. P and Q b. Q and S C. P and S d. R and S nodstumarrow_forward
- An ncRNA may have the following functions: scaffold, guide,alterer of protein function or stability, ribozyme, blocker, and/ordecoy. Which of those functions is/are mediated by each of thencRNAs listed next? (Note: A single ncRNA may have more thanone function.) A. HOTAIRB. RNA of RNasePC. microRNAD. crRNAarrow_forward(a) Match the following list of RNAS (left side) with their function(s) (right side). w. MRNA X. rRNA y. snoRNA Z. snRNA aa, †RNA bb. scaRNA i. block translation of elected mRNAs ii. modification and processing of rRNA iii. modification of snoRNA and snRNA iv. components of ribosome v. spilicing of RNA transcripts vi. directs degradation of selected mRNAs vii. codes for proteins viii. adaptor for protein synthesis cc. miRNA dd. SİRNAarrow_forwardDefine the following terms: a. noncoding RNA b. miRNA c. siRNA d. snoRNA e. lncRNAarrow_forward
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