Campbell Essential Biology with Physiology (6th Edition)
6th Edition
ISBN: 9780134711751
Author: Eric J. Simon, Jean L. Dickey, Jane B. Reece
Publisher: PEARSON
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Textbook Question
Chapter 12, Problem 3SQ
In making recombinant DNA, what is the benefit of using a restriction enzyme that cuts DNA in a staggered fashion?
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In making recombinant DNA, what is the benefit of using a restriction enzyme that cuts DNA in a staggered fashion?
What are the three types of DNA ends that can be generated after cutting DNA with restriction enzymes? What reaction is catalyzed by DNA ligase?
Restriction endonuclease and ligase are two types
of enzymes used in the process of genetic
engineering, i.e., the manipulation of genes. The
restriction endonuclease differs from ligase in that it
breaks the DNA at ends, while ligase causes
the breaks in DNA from interior
joins the fragments of DNA, while ligase
breaks the DNA into fragments
breaks the DNA at specific points, while the
ligase joins the fragments of DNA
breaks the DNA apart at each nucleotide,
while ligase use the pieces to translate
Chapter 12 Solutions
Campbell Essential Biology with Physiology (6th Edition)
Ch. 12 - Suppose you wish to create a large batch of the...Ch. 12 - A carrier that moves DNA from one cell to another,...Ch. 12 - In making recombinant DNA, what is the benefit of...Ch. 12 - A paleontologist has recovered a bit of organic...Ch. 12 - Why do DNA fragments containing STR sites from...Ch. 12 - Prob. 6SQCh. 12 - Prob. 7SQCh. 12 - Name the steps of the whole-genome shotgun method.Ch. 12 - Prob. 9SQCh. 12 - Prob. 10IMT
Ch. 12 - Prob. 11IMTCh. 12 - Prob. 12IMTCh. 12 - Some scientists once joked that when the DNA...Ch. 12 - Prob. 14PSCh. 12 - Listed below are 4 of the 13 genome sites used to...Ch. 12 - In the not-too-distant future, gene therapy may be...Ch. 12 - Today, it is fairly easy to make transgenic plants...Ch. 12 - Prob. 18BSCh. 12 - Prob. 19BS
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- Restriction sites are palindromic; that is, they read the same in the5' to 3' direction on each strand of DNA. What is the advantage ofhaving restriction sites organized this way?arrow_forwardIf you wanted to create recombinant DNA using this enzyme(3' T A 5'), would you have to cut both samples of DNA with this enzyme, or could you use two different restriction enzymes? Explain.arrow_forwardA restriction endonuclease breaks a bacterial plasmid into sticky ends to create recombinant DNA. The same restriction endonuclease is used to cleave the DNA segments that will be added to the plasmid. What are sticky ends, and why are complementary sticky ends on the target DNA and the plasmid it will be inserted into so important?arrow_forward
- What is a restriction digest? What does it mean if you were given a precut DNA?arrow_forwardb) Describe how DNA is digested by different restriction enzymes c) Describe how gel electrophoresis is used to estimate the size of DNA fragments.arrow_forwardIn the formation of recombinant DNA, a restriction endonuclease cuts a bacterial plasmid to give sticky ends. The DNA segments that are to be added to the plasmid are cleaved with the same restriction endonuclease. What aresticky ends and why is it important that the target DNA and the plasmid it will be incorporated into have complementary sticky ends?arrow_forward
- Recombinant DNA construction involves a) Cleaving DNA with a restriction endonuclease and joining with polymerase b) Cleaving and joining DNA with restriction endonuclease c) Cleaving DNA with a restriction endonuclease and joining with ligase Cleaving DNA with ligase and joining with endonuclease d)arrow_forwardWhich restriction enzyme used in your simulated electrophoresis experiment produced DNA with ‘sticky ends’? Which produced blunt ends? Of these two restriction enzymes, which would you choose to use as donor DNA to graft (or splice) onto a recipient strand of DNA, and why?arrow_forwardYou have a recombinant plasmid containing a vector and a segment of foreign DNA, both equal sizes. Draw a picture of this recombinant plasmid labeling foreign and vector regions. Where the foreign DNA meets the vector, there is a cut site for restriction enzyme ABC1. When the recombinant plasmid is cut by ABC1, how many fragments do you expect to be produced? Identify these fragments.arrow_forward
- Which of the following is necessary for a PCR reaction to proceed? a) the sequence of the ends of the DNA to be amplified must be known. b) the sequence of restriction endonuclease recognition sites in the DNA to be amplified and in the plasmid, where the amplified DNA fragment will be cloned must be known. c) The complete sequence of the DNA to be amplified must be known. d) The sequence of restriction endonuclease recognition sites in the DNA to be amplified must be knownarrow_forwardWhen making recombinant DNA, why must you use the same restriction enzyme to cut the gene of interest and the plasmid?arrow_forwardIf the GAATTC palindrome is repeated four times on the same piece of linear DNA, and the restriction enzyme that recognizes that base sequence is present and digests the DNA, how many DNA fragments will be produced?arrow_forward
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