Becker's World of the Cell (9th Edition)
Becker's World of the Cell (9th Edition)
9th Edition
ISBN: 9780321934925
Author: Jeff Hardin, Gregory Paul Bertoni
Publisher: PEARSON
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Chapter 12, Problem 1Q

Based on what you learned in this chapter about the pathway from production of a protein to its secretion, would TIRF be useful if you wanted to trace this entire pathway using a light microscope? Why or why not?

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What differences are there between a free-living, single-celled organism such as a paramecium and a single human cell such as a ciliated cell of the respiratory tract? Use cited.
If there was India Ink added to a culture of Paramecium caudatum, it would increase the average number of food vacuoles because India ink contains no sugar or nutrients and the formation of food vacuoles would be the first stage in the metabolic process when digesting nutrient-rich particles.  If Sugars (sucrose or mannitol) were added to this culture to modify extracellular solute concentration, what effect would we see in the microscopic in regards to the number of food vacuoles? Could sugars have other effects beyond altering solute concentration? Be metabolized?
If there was India Ink added to a culture of Paramecium caudatum, it would increase the average number of food vacuoles because India ink contains no sugar or nutrients and the formation of food vacuoles would be the first stage in the metabolic process when digesting nutrient-rich particles.  If Sugars (sucrose or mannitol) were added to this culture to modify extracellular solute concentration, what effect would we see in the microscopic in regards to the number of food vacuoles and will the amount of food vacuoles change over time? Could sugars have other effects beyond altering solute concentration? Be metabolized?
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