operties of integral membrane proteins can be studied by 1)Atomic force microscopy 2)Cryo-sectioning and electron microscopy 3)Freeze-fracture technique and electron microscopy 4)All of the above
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The properties of integral membrane proteins can be studied by
1)Atomic force microscopy
2)Cryo-sectioning and electron microscopy
3)Freeze-fracture technique and electron microscopy
4)All of the above
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- You are interested in examining the internal structures of a living cell, what type of microscopy will you use? a) phase-contrast microscopy b) bright-field microscopy c) transmission electron microscopy d) scanning electron microscopyWhich of the following microscopes typically requires the use of vital dyes (like methylene blue) to visualize large subcellular structures in a living cell (like the nucleolus or the mitochondrion)? the scanning/tunneling electron microscope (STEM), with good resolution up to about 100,000,000x the transmission electron microscope (TEM), with good resolution up to about 100,000x the scanning electron microscope (SEM), with good resolution up to about 1,000,000x the compound light microscope, with good resolution up to about 1,500x all of the above microscopes would be equally useful in visualizing the interior of organelles1) All of the following are functions of proteins within a membrane except __________. A)Maintaining the fluidity of membranes B)transport ions and other polar substances C)receptors to bond molecules outside of the cell D)helping the body to recognize its own cells 2) Which of the following organelles is not part of the endomembrane system A)mitochondria B)golgi apparatus C)lysosomes D)rough endoplasmic reticulum 3) Which cytoskeletal element is composed of actin and is involved with muscle contraction and provided strength to cells during stretching and compression? A)Microfilimants B)Intermediate filaments C)Microtubules D)Flagella
- Which electron or light microscopy technique would you use for the following? (also explain) For visualizing the fine structure of cellular organelles and cell architecture in the cell interior using very thin cross-sections made in Epon. To obtain sequential images of a macromolecule in order to study its activity in real-time. To obtain an extremely clear three-dimensional image of the nucleus of a single cell. What is the magnification of the image in a microscope using a 20x ocular and a 100x objective?Which type of microscopy allows us to clearly view the internal contents of cell structures smaller than 200 nm?A fluorescent molecule of 2000 daltons is microinjected into a single cell of an epithelial layer. When the layer is examined in a fluorescence microscope, the molecule remains confined to that cell. However, when a fluorescent molecule of 800 daltons is injected into a single cell of an epithelial layer, it quickly appears in the adjacent cells. Which of the following is responsible for the spread of the smaller molecule? 1. A) Desmosomes (maculae adherentes) 2. B) Focal contacts 3. C) Gap junctions 4. D) Intermediate junctions 5. E) Tight junctions (zonulae occludentes)
- After investigation of a specimen under light microscope and electron microscope, numerous amounts of spherical shaped particles were seen in the electron microscope while this type of structure was not visible under the light microscope. Further characterization revealed that these particles lack cellular organelles (like the nucleus, mitochondria…). Considering the above circumstance, answer the following questions. (A) What do you think would be the size of the specimen? (B) What do you suspect these particles are most likely to be? (C) Would you define these particles as living? Why/why not-justify your answer briefly12) Match the cell-cell junction on the left with the appropriate function on the right. Note that more than one letter may apply for a particular function and that each letter may be used more than once or not at all. A) Gap junctions provides a very strong attachment point between cells B) Desmosomes allow easy movement of large amounts of substances between adjacent cells C) Plasmodesmata seals the plasma membranes of adjacent cells together, preventing the movement of molecules between them D) Tight junctionsWhich term best describes the following scenario: Calcium ions travel through the cell membrane into the cell, using an integral protein. There is a higher concentration of calcium in the cell. A) Diffusion/Passive transport B) Facilitated diffusion C) Active transport D) Fluid mosaic model E) All terms in this list are correct
- (a) If you were to magnify a cell 10,000-fold (typical of the magnification achievedusing an electron microscope), how big would it appear? Assume you are viewing a“typical” eukaryotic cell with a cellular diameter of 50 μm.(b) If this cell were a muscle cell (myocyte), how many molecules of actin could ithold? Assume the cell is spherical and no other cellular components are present; actinmolecules are spherical, with a diameter of 3.6 nm. (c) If this were a liver cell (hepatocyte) of the same dimensions, how manymitochondria could it hold? Assume the cell is spherical; no other cellular components arepresent; and the mitochondria are spherical, with a diameter of 1.5 μmWhich of the below statements correctly describes the extracellular matrix? a)A layer that facilitates connections and communications with other cells in a tissue b)A gel like substance that resists compression c)All of the other answers are correct d)a complex mix of proteins and polysaccharides.Pick the best form of microscopy to use in each situation, each answer used only once: You want to figure out if a bacterium can ✓ [Choose ] swim in a liquid medium You want to visualize the repeating crystalline structure of the S-layer You want to identify the basic cell wall structure of an unknown bacterium You want to watch proteins of the divisome to see how they change their location in dividing cells You want to visualize a single membrane protein complex on the surface of a cell Atomic Force Microscopy Phase-contrast microscopy Light microscopy (with staining) Fluorescence microscopy Electron microscopy [Choose] [Choose] [Choose ]