Biology
5th Edition
ISBN: 9781260487947
Author: BROOKER
Publisher: MCG
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Textbook Question
Chapter 11, Problem 6TY
Meselson and Stahl were able to demonstrate semiconservative replication in E. coli by
- a. using radioactive isotopes of phosphorus to label the original strand and visually determining the relationship of original and new DNA strands.
- b. using different enzymes to eliminate old strands from DNA.
- c. using isotopes of nitrogen to label the DNA and determining the relationship of original and new DNA strands by density differences of the new DNA molecules.
- d. labeling viral DNA before it was incorporated into a bacterial cell and visually determining the location of the DNA after centrifugation.
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You are studying a colony of cells and determine that some of these cells have a mutated DNA polymerase I that results in loss of function of this enzyme.
A) What will the effect of the mutation in DNA polymerase I be on DNA replication? In your answer make sure to describe what would be observed in the leading and lagging strand and explain your reasoning.
B) Will this mutation in DNA polymerase I have an impact on another step in DNA replication? In your answer make sure to indicate whether DNA replication will be impacted or not. If it is not, explain why. If it is impacted, then describe the step that is impacted and name the molecule or enzyme involved.
Which of the following statements BEST DESCRIBES the main findings of the Meselson-Stahl experiment?
A.
DNA can be separated using centrifugation
B.
The semiconservative model of DNA replication is more accurate than the dispersive or conservative models of DNA replication
C.
Using 14N in experiments is an effective way of tracking nitrogen molecules
D.
Bacteria grown in the presence of a heavier nitrogen isotope (15N) will replicate at a slower rate than those that utilise a lighter nitrogen isotope (14N)
E.
Both strands of each new DNA double helix are brand new and synthesized from individual nucleotides
Which of the following best describes the process of DNA seqencing.
a. DNA is seperated on a gel and the different bands are labled with flouroscent nucleotides and scanned with a laser.
b. A laser is used to flurorescently label the nucleotides present with in the DNA , the DNA is run on a gel and then the DNA is droken into fragments
c. Nucleotides are scanned with a laser and incrprorated into the DNA that has been seperated on a gel and then DNA is amplified with PCR.
d. fragments of DNA are produced in a reaction that lables them with any of four different fluroscent dyes and the fragmented then are run on a gel and scanned with laser
e. DNA is broken down into its constituents nucleotides and the nucleotides are then run on a gel and purified with a laser
Chapter 11 Solutions
Biology
Ch. 11.1 - Prob. 1CSCh. 11.1 - Prob. 1EQCh. 11.1 - Prob. 2EQCh. 11.1 - CoreSKILL In the experiment of Avery, MacLeod,...Ch. 11.2 - Prob. 1CCCh. 11.2 - Prob. 2CCCh. 11.2 - Core Skill: Modeling The goal of this modeling...Ch. 11.2 - Prob. 2CSCh. 11.3 - If this experiment was conducted for four rounds...Ch. 11.4 - Molecular Mechanism of DNA Replication Concept...
Ch. 11.4 - Prob. 2CCCh. 11.4 - Prob. 3CCCh. 11.4 - Prob. 4CCCh. 11.5 - Prob. 1CCCh. 11.5 - Prob. 1CSCh. 11 - Why did researchers initially believe that the...Ch. 11 - Prob. 2TYCh. 11 - Which of the following equations is accurate...Ch. 11 - Prob. 4TYCh. 11 - Which of the following statements about the...Ch. 11 - Meselson and Stahl were able to demonstrate...Ch. 11 - During replication of a DNA molecule, the daughter...Ch. 11 - Prob. 8TYCh. 11 - A nucleosome is a. a dark-staining body composed...Ch. 11 - The conversion of euchromatin into heterochromatin...Ch. 11 - What are the four key criteria that the genetic...Ch. 11 - A double-stranded DNA molecule contains 560...Ch. 11 - Prob. 3CQCh. 11 - Prob. 1COQCh. 11 - CoreSKILL How might you provide evidence that DNA...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- a) Explain semi-conservative modelmof DNA replication b) discuss initiation in DNA replication and explain why it is a tightly controlled process.arrow_forwardConsidering the Polymerase Chain Reaction (PCR), we can state that: a) It is a technique that promotes linear amplification of the number of DNA molecules in a sample. b) Depends on an initial template which can be a DNA or RNA molecule c) The reaction must contain, in addition to the template sequence, nitrogenous bases, sense and antisense primers and DNA polymerase enzyme. d)The primer sense rings on the template strand 3’>5’, extending from the 3’ end e)arrow_forwardDescribe the main jobs of each of the following enzymes in the E. coli model of replication: A. DNA Polymerase I B. DNA Polymerase II C. DNA Polymerase IIIarrow_forward
- a) "Out of three E.coli DNA polymerases, DNA polymerases 3 has a high processivity and rate of polymerization and therefore better suited for replication of the genome" What is meant by processivity? how does the DNA polymerase 3 maintain high processivity? b) What is a replication fork ?. Give the protein/enzymes of a replication fork and describe their function?arrow_forwardWhat was the significance of Meselson and Stahl’s experiments on DNA replication using the heavy isotope of Nitrogen? A. telomerase was identified as the molecule responsible for solving the end replication problem of eukaryotic chromosomes B. the existence of lagging strand synthesis was proven. C. the rate of DNA synthesis by DNA polymerase was measured D. the processivity of DNA polymerase was established E. the semi-conservative mode of DNA replication was confirmedarrow_forwardA.) Damaged DNA can be reversed if nucleotides can be replaced with a proper nucleotide for a correct amino acid base. B.) Direct reversal of DNA repair can destroy the abnormal bonds of the nucleotides in the DNA sequence. a. Statement A is correct b. Statement B is correct c. Both A and B are correct d. Both A and B are incorrectarrow_forward
- Which of the following statements is TRUE concerning the synthesis of the leading and lagging strands of DNA in prokaryotic cells? a. O b. The leading strand is synthesized by one polymerase III continuously, and the lagging strand is synthesized by several molecules of DNA polymerase III. d. The leading and lagging strands are synthesized at the same time by the one DNA polymerase I. O c. The leading and lagging strands are synthesized at the same time by the one DNA polymerase III. The leading strand is synthesized by one polymerase III, and the lagging strand is synthesized by DNA polymerase I.arrow_forwardWhich of the following is the most accurate description of the E.coli replisome? a. Clamp loader positions two Pol III core polymerases on the leading strand and one Pol III core polymerase on the lagging strand b. Single strand template DNA forms two loops on the lagging strand while two Pol III core polymerases synthesize Okazaki fragments c. Okazaki fragments are synthesized in the opposite direction to movement of the replication fork d. Clamp loader only connects with Pol III core polymerases associated with the lagging strand e. Both b. and c. are accuratearrow_forwardMeselson and Stahl grew bacteria in medium containing heavy nitrogen. After many celldivisions, the bacteria were removed, washed and allowed to continue growth in mediumcontaining normal (light) nitrogen.a. In this experiment, what part of the DNA gets labeled with heavy nitrogen?arrow_forward
- It is desired to isolate genomic DNA from liquid culture of S. cerevisiae yeast. A commercial kit will be used to isolate genomic DNA from this liquid culture. Answer the following questions to understand the strategy used by commercial kits for genomic DNA isolation. a) List all the steps from cell pellet preparation to DNA elution. b) With which feature can the membrane in the column that comes with the commercial kit bind DNA? c) Which component in the kit would you use to recover the DNA from the membrane of the column to which the DNA was attached?arrow_forwarda) If you isolated DNA from the ear and the tail of the same mouse, would you expect the DNA, isolated from the two tissue types, to be the same? Why? b) Provide one difference between DNA replication in eukaryotes and prokaryotes with regard to their origin (s) of replication.arrow_forwardCompare and contrast the properties of the enzymes DNA polymerase I and polymerase III from E. coli.arrow_forward
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