Biology
Biology
5th Edition
ISBN: 9781260487947
Author: BROOKER
Publisher: MCG
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Chapter 11.2, Problem 1CS

Core Skill: Modeling The goal of this modeling challenge is to predict the hydrogen-bonding relationship between O6-MeG and cytosine.

Chapter 11.2, Problem 1CS, Core Skill: Modeling The goal of this modeling challenge is to predict the hydrogen-bonding

Modeling Challenge: As discussed in Chapter 15, certain chemicals, such as nitrogen mustard and ethyl methanesulfonate, can modify the structures of DNA bases. For example, a methyl group (─CH3) can be attached to the oxygen atom on guanine, thereby creating 6-O-methylguanine (O6-MeG), as shown to the right. When O6-MeG is included in a DNA strand, it can form only two hydrogen bonds with cytosine instead of three. Draw a model for the base pairing between O6-MeG and cytosine.

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Original sequence:    Consider the following coding 71 nucleotide DNA template sequence (It does not contain a translational start):  5’-GTTTCCCCTATGCTTCATCACGAGGGCACTGACATGTGTAAACGAAATTCCAACCTGAGCGGCGT GTTGAG-3’    Question:    4) In a mutant you discovered that the underlined nucleotide has  been deleted. What would the resulting peptide sequence be? What  type of mutation is this?  5’-GTTTCCCCTATGCTTCATCACGAGGGCACTGACATGTGTAAACGAAATTCCAACCTGAGCGGCGT GTTGAG-3
Using Fig. as a guide, draw the complete structure of a nucleoside triphosphate before and after it becomes incorporated into a polynucleotide chain. Draw the structure that would result if the newly formed phosphodiester bond were hydrolyzed.
DNA contents of nitrogenous bases • %A = %T %C = %G • A+G = C+T %3D Example: if 35% of the bases of a DNA - molecule is thymine what the % of Cyosine?
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