Which of the following statements is TRUEconcerning the synthesis of the leading andlagging strands of DNA in prokaryotic cells?a.O b.The leading strand is synthesized by onepolymerase III continuously, and the laggingstrand is synthesized by several molecules of DNApolymerase III.d.The leading and lagging strands are synthesizedat the same time by the one DNA polymerase I.O c. The leading and lagging strands are synthesizedat the same time by the one DNA polymerase III.The leading strand is synthesized by onepolymerase III, and the lagging strand issynthesized by DNA polymerase I.

DNA replication is a process by which one molecule of DNA is duplicated to 2 molecules of DNA. The…

Answered: Which of the following statements is…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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It is essential that RNA primers at the ends of Okazakifragments be removed and replaced by DNA becauseotherwise which of the following events would result?a. The RNA would interfere with topoisomerasefunction.b. The RNA would be more likely to contain errorsbecause primase lacks a proofreading function.c. The β-clamp of the DNA pol II dimer would releasethe DNA and replication would stop.d. The RNA primers would be likely to hydrogen bondto each other, forming complex structures that mightinterfere with the proper formation of the DNA helix
The function of gamma (y) complex of DNA polymerase III is to Select one: O a. to unwind double strand DNA (template DNA). O b. to associate the core DNA polymerase llI with template DNA. O c. to stimulate 5' to 3' exonuclease activity O d. to upload beta subunit of DNA polymerase III to template DNA.
Consider the following segment of DNA:5′ GCTTCCCAA 3′3′ CGAAGGGTT 5′Assume that the top strand is the template strand usedby RNA polymerase.a. Draw the RNA transcribed.b. Label its 5′ and 3′ ends.c. Draw the corresponding amino acid chain.d. Label its amino and carboxyl ends.Repeat parts a through d, assuming the bottom strand tobe the template strand.
Defects in which enzymes enumerated below would impair the repair of the sequence below in Eukaryotic cells (select all that apply)? GCTATGACTUGCATCCGAT CGATACTGAGCGTAGGCTA A. uvrA✔ B. Uracil DNA Glycosylase✔ C. MutS✔ D. Flap Endonuclease (FEN1) ✔ E. DNA Polymerase Beta
The DNA polymerase I uses its to remove the RNA primers during DNA replication in E. coli cells. Select one: a. 3' to 5' exonuclease activity O b. 3' to 5' DNA helicase activity O c. 5' to 3' exonuclease activity O d. 5' to 3' DNA polymerase activity
a. RNA Polymerase b. DNA Polymerase I c. DNA Polymerase III d. Single Strand binding protein e. Sigma Factor f. Ligase g. Peptidyl transferase h. Primers i. Aminoacyl synthetase j. Gyrase k. Release factors I. Rho factors m. AUG-fmet n. Initiation Factors Identify the following: 1. Catalyzes peptide bond formation between amino acids 2. Binds the amino acid to tis corresponding TRNA 3. Recognizes terminator region 4. Relaxes the tension created by the unwinding process. 5. Complements DNA nucleotides with RNA nucleotides. 6. Complements DNA nucleotides with deoxynucleotide triphosphates. 7. Recognizes UAA, UAG, UGA
Which of the following statements is FALSE regarding the molecular mechanism for DNA polymerases? A. The active site contains 2 divalent metal ions B. A single stranded DNA template is required C. The enzyme can only attach a new deoxynucleotide to the 5’ end of a growing chain D. The 3’OH on the deoyxyribose ring attacks a phosphate of a dNTP to produce a new phosophodiester bond E. None of the above (all are true statements)
Different DNA polymerases play distinct roles in DNA replication and repair in both prokaryotic and eukaryotic cells. All known DNA polymerases synthesize DNA only in by the addition of the dNTPs to a performed primer strand of DNA. A. positive direction B. 3' to 5' direction C. 5' to 3' direction D. negative direction
Please consider figures 1 and 2 below. 5' A B 5' -I H. D. Figure 1: Replication Figure 2: Transcription a. Which letters, A to J represent DNA strands that will be synthesized continuously? b. At which letter, A to J, are ribonucleotides being added? c. Name and describe the process that occurs in prokaryotes as the synthesized mRNA hangs free of the double helix while transcription continues. d. In what direction will the replication bubble move in figure 1 as replication continues? Rleace Ibol ancwors (a) to (d)
A mutant DNA strand was transcribed then translated to proteins. a. What is the protein product of the mutant DNA strand? The sequence of the mutant strand is shown below: 5'-TGCCATAACTGTTCGTACTGGCAAATTGCC-3' 3'-ACGGTATTGACAAGCATGACCGTTTAACGG-5' b. The mutation altered the sequence of the wild type template DNA such that a degenerate codon for a basic amino acid in the wild type was converted to a non-degenerate codon resulting in the sequence for the mutant strand shown. What was the original amino acid? c. Compare the charges and pl of the mutant peptide and the normal (wild- type) peptide at physiological pH?
You are characterizing a new DNA polymerase. In a test tube, you incubate the enzyme with all the components needed for catalysis, EXCEPT you don't add any dNTPs. However, you notice that some type of catalysis has occurred. What is the most likely product in this case? O The supercoils in the DNA have been relieved O The sugar has been converted from a deoxyribose to a ribose A DNA strand is one base shorter due to nuclease activity The magnesium ion has been converted to a charge of +2 to +1
What does it mean to say thhat extension by DNA polymerase III proceed 5'---3'? A. The 5' end of a DNA polymerase molecule attaches to the 3' end of primase. B. DNA polymerase adds nucleotides to a growing strand, moving in the 5' to 3' direction. C. DNA polymerase seals nicks as it moves along a DNA strand toward the 3' end. D. DNA polymerase can only synthesize DNA at the 5' end of an existing strand of DNA.

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