Becker's World of the Cell (9th Edition)
Becker's World of the Cell (9th Edition)
9th Edition
ISBN: 9780321934925
Author: Jeff Hardin, Gregory Paul Bertoni
Publisher: PEARSON
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Chapter 11, Problem 11.3PS
Summary Introduction

To describe: Two reasons why plants do not manage their energy economy such that they directly translocate ATP, without the use of the Calvin Cycle, the glycolytic pathway or the citric acid cycle.

Introduction: Photosynthesis, respiration and other such processes manage the energy flow in plants. Energy is converted and it flows through these cycles to provide food, oxygen and other such compounds to all parts of the plant, to maintain the processes of the cell. Carbon dioxide and water make glucose, which is the food for the cells of the plant.

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Fo-F1 ATPase. The energy for ATP synthesis from ADP and Pi is provided by the downhill transport of protons through the rotary FoF1 ATP synthase (lecture 22).   The enzyme has 3 a-b and 12 ‘c’ subunits. The mitochondrion maintains Df=180 mV (negative inside), pHin = 8, pHout=7, [Pi] = 3 mM and ADP is present as well. How much energy is available (from the proton electrochemical gradient) for ATP synthesis under these conditions (in kJ/mol)?                                                                                                                  What [ATP]/[ADP] ratio will be established at steady-state under these conditions?                                                                                                                                                What would be the [ATP]/[ADP] ratio if the enzyme had only 9 ‘c’ subunits? Remember that full revolution of the crank (gamma subunit) produces 3 ATP.
ATP yield. Each of the following molecules is processed by glycolysis to lactate. How much ATP is generated from each molecule?
Fo-F1 ATPase. The energy for ATP synthesis from ADP and Pi is provided by the downhill transport of protons through the rotary FoF1 ATP synthase . The enzyme has 3 alpha-beta and 12 ‘c’ subunits. The mitochondrion maintains change in membrane potential=180 mV (negative inside), pHin = 8, pHout=7, [Pi] = 3 mM and ADP is present as well. . What [ATP]/[ADP] ratio will be established at steady-state under these conditions?  What would be the [ATP]/[ADP] ratio if the enzyme had only 9 ‘c’ subunits? full revolution of the crank (gamma subunit) produces 3 ATP.
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