Foundations in Microbiology
10th Edition
ISBN: 9781259705212
Author: Kathleen Park Talaro, Barry Chess Instructor
Publisher: McGraw-Hill Education
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Chapter 10.L1, Problem 3WC
Summary Introduction
To analyze:
Why it would be an advantage to synthesize eukaryotic genes using reverse transcriptase.
Introduction:
Reverse transcriptase is an RNA-dependent DNA polymerase. This enzyme can use RNA molecules as the template to synthesize DNA. They are found in retroviruses.
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Why is cDNA used for cloning?
Why can’t a researcher use a poly-T primer in a reverse transcriptase reaction that utilizes prokaryotic mRNA as a template?
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Chapter 10 Solutions
Foundations in Microbiology
Ch. 10.1 - Define genetic engineering, and describe some of...Ch. 10.1 - Explain the properties of DNA that lend to its...Ch. 10.1 - Summarize the major methods of analyzing DMA and...Ch. 10.1 - Describe the technology behind Identifying,...Ch. 10.1 - Define genetic engineering and biotechnology, and...Ch. 10.1 - Describe the processes involved in denaturing and...Ch. 10.1 - Define restriction endonuclease and explain what...Ch. 10.1 - Prob. 4CYPCh. 10.1 - Explain how electrophoresis works and the general...Ch. 10.1 - How would you make a copy of DNA from an mRNA...
Ch. 10.1 - Briefly summarize the steps involved in DNA...Ch. 10.1 - Outline the steps in the PCR technique and...Ch. 10.1 - What are the functions of primer and Taq...Ch. 10.2 - Explain what is involved in recombinant DNA...Ch. 10.2 - Characterize the events in cloning, using an...Ch. 10.2 - List and discuss some protein products of...Ch. 10.2 - What characteristics of plasmids and...Ch. 10.2 - Name several types of vectors, and list the types...Ch. 10.2 - Describe the basic principles behind recombinant...Ch. 10.2 - Summarize the characteristics of bacteria and...Ch. 10.2 - Outline the main steps in cloning a gene,...Ch. 10.2 - What is one way to determine whether a bacterial...Ch. 10.2 - Characterize several products that have resulted...Ch. 10.3 - Define what is meant by the term transgenic or...Ch. 10.3 - Describe the uses of genetically modified bacteria...Ch. 10.3 - Prob. 10ELOCh. 10.3 - Explain how DNA technology can be used to treat...Ch. 10.3 - Describe several uses of genetically modified...Ch. 10.3 - Prob. 18CYPCh. 10.3 - Why must animals usually be modified in the embryo...Ch. 10.3 - Prob. 20CYPCh. 10.3 - What are some ethical and biological...Ch. 10.3 - Outline the uses of gene therapy and gene editing...Ch. 10.4 - Outline the uses of gene therapy and gene editing...Ch. 10.4 - Describe two methods in performing a DNA analysis,...Ch. 10.4 - Describe several applications of DNA profiling and...Ch. 10.4 - Describe what a DNA profile is and how STRs and...Ch. 10.4 - Prob. 24CYPCh. 10.4 - Explain the origins of mtDNA and its importance in...Ch. 10.4 - Explain the difference between a DNA profile and a...Ch. 10.L1 - Which gene is incorporated into plasmids to detect...Ch. 10.L1 - Which of the following is not essential to carry...Ch. 10.L1 - Which of the following is not a part of the Sanger...Ch. 10.L1 - The function of ligase is to a. rejoin segments of...Ch. 10.L1 - The pathogen of plant roots that is used as a...Ch. 10.L1 - Prob. 6MCQCh. 10.L1 - Which DNA fragment will be closest to the top...Ch. 10.L1 - Prob. 8MCQCh. 10.L1 - For which of the following would not require a...Ch. 10.L1 - Prob. 10MCQCh. 10.L1 - What type of mutation caused Nicholas’s disease?...Ch. 10.L1 - Which type of cells were used to extract the DNA...Ch. 10.L1 - Lay out the genetics of Nicholas’s case,...Ch. 10.L1 - Prob. 1WCCh. 10.L1 - What is it about the endonucleases that prevents...Ch. 10.L1 - Prob. 3WCCh. 10.L1 - a. Explain what hybridization is and how it is...Ch. 10.L1 - Prob. 5WCCh. 10.L1 - Prob. 6WCCh. 10.L1 - Prob. 7WCCh. 10.L1 - Explain the kinds of study involved in genomics,...Ch. 10.L1 - For what reasons would gene therapy be more...Ch. 10.L1 - Prob. 10WCCh. 10.L2 - a. Give an example of a benefit of genetic...Ch. 10.L2 - a. When gene probes, DNA profiling, and sequencing...Ch. 10.L2 - Which suspect is the likely perpetrator according...Ch. 10.L2 - Trace the genetic steps in the development of a...Ch. 10.L2 - You are on a jury to decide whether a person...Ch. 10.L2 - Can you think of some reasons it would not be...Ch. 10.L2 - What would be some major impediments to...Ch. 10.L2 - Prob. 8CTCh. 10.L2 - Describe the main differences between genome...Ch. 10.L2 - Itemize all of the ways that microbes have...Ch. 10.L2 - Below are two unrelated DNA paternity tests: one...Ch. 10.L2 - Figure 9.25d, shown here, shows the original...
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- Why do we convert RNA to cDNA?arrow_forwardTransduction is sometimes described as a mistake in the bacteriophage reproductive cycle. Explain how it can be viewed as a mistake?arrow_forwardGenetic transfer via transformation can also be used to map genes along the bacterial chromosome. In this approach, fragments of chromosomal DNA are isolated from one bacterial strain and used to transform another strain. The experimenter examines the transformed bacteria to see if they have incorporated two or more different genes. For example, the DNA may be isolated from a donor E. coli bacterium that has functional copies of the araB and leuD genes. Let’s call these genes araB+ and leuD+ to indicate the genes are functional. These two genes are required for arabinose metabolismand leucine synthesis, respectively. To map the distance betweenthese two genes via transformation, a recipient bacterium is used that is araB− and leuD−. Following transformation, the recipient bacterium may become araB+ and leuD+. This phenomenon is calledcotransformation because two genes from the donor bacterium have been transferred to the recipient via transformation. In this type of experiment, the…arrow_forward
- Genetic transfer via transformation can also be used to map genes along the bacterial chromosome. In this approach, fragments of chromosomal DNA are isolated from one bacterial strain and used to transform another strain. The experimenter examines the transformed bacteria to see if they have incorporated two or more different genes. For example, the DNA may be isolated from a donor E. coli bacterium that has functional copies of the araB and leuD genes. Let’s call these genes araB+ and leuD+ to indicate the genes are functional. These two genes are required for arabinose metabolismand leucine synthesis, respectively. To map the distance betweenthese two genes via transformation, a recipient bacterium is used that is araB− and leuD−. Following transformation, the recipient bacterium may become araB+ and leuD+. This phenomenon is calledcotransformation because two genes from the donor bacterium have been transferred to the recipient via transformation. In this type of experiment, the…arrow_forwardGenetic transfer via transformation can also be used to map genes along the bacterial chromosome. In this approach, fragments of chromosomal DNA are isolated from one bacterial strain and used to transform another strain. The experimenter examines the transformed bacteria to see if they have incorporated two or more different genes. For example, the DNA may be isolated from a donor E. coli bacterium that has functional copies of the araB and leuD genes. Let’s call these genes araB+ and leuD+ to indicate the genes are functional. These two genes are required for arabinose metabolismand leucine synthesis, respectively. To map the distance betweenthese two genes via transformation, a recipient bacterium is used that is araB− and leuD−. Following transformation, the recipient bacterium may become araB+ and leuD+. This phenomenon is calledcotransformation because two genes from the donor bacterium have been transferred to the recipient via transformation. In this type of experiment, the…arrow_forwardStarting with a sample of RNA that contains the mRNA for theβ-globin gene, explain how you could create many copies of theβ-globin cDNA using reverse transcriptase PCR.arrow_forward
- If I clone a complete eukaryotic gene, including the eukaryotic promoter region, ligate it into a plasmid, and transform it into E. coli, will I be able use the transformed E. coli to make the corresponding protein? Explain why, or why not? If you decide to do this, what would your cloning strategy be?arrow_forwardYou are on a research project to study rice. Your want to use reverse transcription PCR to amplify the OsMEI28 cDNA from the plant and create a plasmid that contains the cDNA sequence in a vector. Briefly describe the steps that you would need to take.arrow_forwardWhy is the genome contained within a membrane?arrow_forward
- Sketch a figure to show how reverse transcription is catalyzed by the enzyme reverse transcriptase. Label the nucleic acids involved and produced by the process and draw arrows to indicate the direction of synthesis along the template.arrow_forwardWhen using bacteria to produce a particular protein, why is it usually better to use the cDNA version of the gene rather than the genomic DNA version?arrow_forwardHow to incorporate prokaryotic gene into eukaryotic cell to make an transgenic organism?In this case what promoter will be used.explain the process in detailsarrow_forward
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