Foundations in Microbiology
10th Edition
ISBN: 9781259705212
Author: Kathleen Park Talaro, Barry Chess Instructor
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 10.2, Problem 13CYP
Summarize the characteristics of bacteria and yeasts that make them good cloning hosts.
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PLease explain What characteristics of plasmids makes them good cloning vectors? Discuss
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Briefly present experimental and practical benefits of using PCR in DNA cloning process. Give some examples of clinical applications.
Chapter 10 Solutions
Foundations in Microbiology
Ch. 10.1 - Define genetic engineering, and describe some of...Ch. 10.1 - Explain the properties of DNA that lend to its...Ch. 10.1 - Summarize the major methods of analyzing DMA and...Ch. 10.1 - Describe the technology behind Identifying,...Ch. 10.1 - Define genetic engineering and biotechnology, and...Ch. 10.1 - Describe the processes involved in denaturing and...Ch. 10.1 - Define restriction endonuclease and explain what...Ch. 10.1 - Prob. 4CYPCh. 10.1 - Explain how electrophoresis works and the general...Ch. 10.1 - How would you make a copy of DNA from an mRNA...
Ch. 10.1 - Briefly summarize the steps involved in DNA...Ch. 10.1 - Outline the steps in the PCR technique and...Ch. 10.1 - What are the functions of primer and Taq...Ch. 10.2 - Explain what is involved in recombinant DNA...Ch. 10.2 - Characterize the events in cloning, using an...Ch. 10.2 - List and discuss some protein products of...Ch. 10.2 - What characteristics of plasmids and...Ch. 10.2 - Name several types of vectors, and list the types...Ch. 10.2 - Describe the basic principles behind recombinant...Ch. 10.2 - Summarize the characteristics of bacteria and...Ch. 10.2 - Outline the main steps in cloning a gene,...Ch. 10.2 - What is one way to determine whether a bacterial...Ch. 10.2 - Characterize several products that have resulted...Ch. 10.3 - Define what is meant by the term transgenic or...Ch. 10.3 - Describe the uses of genetically modified bacteria...Ch. 10.3 - Prob. 10ELOCh. 10.3 - Explain how DNA technology can be used to treat...Ch. 10.3 - Describe several uses of genetically modified...Ch. 10.3 - Prob. 18CYPCh. 10.3 - Why must animals usually be modified in the embryo...Ch. 10.3 - Prob. 20CYPCh. 10.3 - What are some ethical and biological...Ch. 10.3 - Outline the uses of gene therapy and gene editing...Ch. 10.4 - Outline the uses of gene therapy and gene editing...Ch. 10.4 - Describe two methods in performing a DNA analysis,...Ch. 10.4 - Describe several applications of DNA profiling and...Ch. 10.4 - Describe what a DNA profile is and how STRs and...Ch. 10.4 - Prob. 24CYPCh. 10.4 - Explain the origins of mtDNA and its importance in...Ch. 10.4 - Explain the difference between a DNA profile and a...Ch. 10.L1 - Which gene is incorporated into plasmids to detect...Ch. 10.L1 - Which of the following is not essential to carry...Ch. 10.L1 - Which of the following is not a part of the Sanger...Ch. 10.L1 - The function of ligase is to a. rejoin segments of...Ch. 10.L1 - The pathogen of plant roots that is used as a...Ch. 10.L1 - Prob. 6MCQCh. 10.L1 - Which DNA fragment will be closest to the top...Ch. 10.L1 - Prob. 8MCQCh. 10.L1 - For which of the following would not require a...Ch. 10.L1 - Prob. 10MCQCh. 10.L1 - What type of mutation caused Nicholas’s disease?...Ch. 10.L1 - Which type of cells were used to extract the DNA...Ch. 10.L1 - Lay out the genetics of Nicholas’s case,...Ch. 10.L1 - Prob. 1WCCh. 10.L1 - What is it about the endonucleases that prevents...Ch. 10.L1 - Prob. 3WCCh. 10.L1 - a. Explain what hybridization is and how it is...Ch. 10.L1 - Prob. 5WCCh. 10.L1 - Prob. 6WCCh. 10.L1 - Prob. 7WCCh. 10.L1 - Explain the kinds of study involved in genomics,...Ch. 10.L1 - For what reasons would gene therapy be more...Ch. 10.L1 - Prob. 10WCCh. 10.L2 - a. Give an example of a benefit of genetic...Ch. 10.L2 - a. When gene probes, DNA profiling, and sequencing...Ch. 10.L2 - Which suspect is the likely perpetrator according...Ch. 10.L2 - Trace the genetic steps in the development of a...Ch. 10.L2 - You are on a jury to decide whether a person...Ch. 10.L2 - Can you think of some reasons it would not be...Ch. 10.L2 - What would be some major impediments to...Ch. 10.L2 - Prob. 8CTCh. 10.L2 - Describe the main differences between genome...Ch. 10.L2 - Itemize all of the ways that microbes have...Ch. 10.L2 - Below are two unrelated DNA paternity tests: one...Ch. 10.L2 - Figure 9.25d, shown here, shows the original...
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- Differentiate between plasmids, phage-based cloning vectors, cosmids, and artificial chromosomes in terms of structure and applicationarrow_forwardGive three important characteristics of cloning vectors.arrow_forwardCloning vectors are not just limited to bacterial plasmids. Bacteriophages and M13 phage vectors are also commonly utilized in the cloning process. State any five (5) key criteria to be an effective cloning vector.arrow_forward
- Compare the properties of cloning vectors such as pUC18, bacteriophage λ, and BACs.arrow_forwarddescribe how the bacteria should have the ability to be recovered back from the infected host for the purpose of experimentation including sequencing ,vitro culturing and polymerase chain reaction amplification.arrow_forwardWhat is happening in each steps in standard method of molecular cloning. 1: choosing vector and clone host. 2: Preparing vector DNA 3: Preparing DNA to be cloned 4: creating a rDNA (recombinant DNA) 5: Introducing rDNA into the selected host. 6: selecting organisms that contain rDNA. 7: screening for the clones with desired biological properties and DNA inserts.arrow_forward
- Recombinant DNA techniques are used to custom –build bacteria for two main reasons: to make a multiple copies of desired genes and to obtain useful proteins. Give an example of each of these applications in the field of pharmaceutical, medicine and agriculture.arrow_forwardExplain how biotechnology and bioinformatics are used to identify microorganisms Explain why biotechnology has replaced many of the traditional diagnostic tools in microbiology Explain why a PCR reaction needs a source of nucleotides in the form of ATP, GTP, CTP and TTP. Explain what primers are and describe their use in PCR reactions Given the results from a PCR/Gel electrophoresis or a DNA sequence; determine the identity of a bacterium or virus...arrow_forwardExplain how reverse-transcriptase PCR is carried out.arrow_forward
- Explain how and why PCR can be used to amplify DNA. Describe the steps in the process. Be sure to address the role of Taq DNA polymerase.arrow_forwardWhat are secondary metabolites? In terms of biotechnology, how do we get bacteria to produce them with cloning technology? Explain the stages in schematics and explanations.arrow_forwardDescribe how Restriction Enzymes, Plasmids, DNA Hybridization, and PCR can be used for molecular cloning and genetic engineering.arrow_forward
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