Microbiology: A Systems Approach
5th Edition
ISBN: 9781259706615
Author: Marjorie Kelly Cowan Professor
Publisher: McGraw-Hill Education
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Chapter 10.2, Problem 4AYP
Summary Introduction
To enlist:
The steps in the polymerase chain reaction and also discuss one disadvantage to the PCR technique.
Concept introduction
PCR is a technique used to replicate and produce various copies of DNA. PCR has a wide application in research, medicine, agriculture and many other fields.
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Briefly describe the polymerase chain reaction. What is the difference between a step and a cycle?
Outline the essential steps in the polymerase chain reaction.
List the fundamental steps of the polymerase chain reaction.
Chapter 10 Solutions
Microbiology: A Systems Approach
Ch. 10.1 - Provide examples of practical applications of...Ch. 10.2 - Prob. 2AYPCh. 10.2 - Describe how gel electrophoresis is used to...Ch. 10.2 - Prob. 4AYPCh. 10.2 - Prob. 5AYPCh. 10.3 - Prob. 6AYPCh. 10.3 - List examples of genetically modified bacteria,...Ch. 10.4 - Prob. 8AYPCh. 10.4 - Prob. 9AYPCh. 10.5 - Outline in general terms the process of DNA...
Ch. 10.5 - Prob. 11AYPCh. 10.5 - Prob. 12AYPCh. 10.5 - Prob. 13AYPCh. 10.6 - Prob. 14AYPCh. 10 - Which of the following is/are not essential to...Ch. 10 - Prob. 2MCQCh. 10 - The function of ligase is to a. rejoin segments of...Ch. 10 - The creation of biological molecules entirely from...Ch. 10 - Which of the following sequences, when combined...Ch. 10 - A region of DNA in a plasmid that is recognized by...Ch. 10 - Prob. 7MCQCh. 10 - Which of the following is a primary participant in...Ch. 10 - Single nucleotide polymorphisms are found in a....Ch. 10 - Microarrays are used to monitor a. the rate of DNA...Ch. 10 - Prob. 11TFCh. 10 - A nucleic acid probe can be used to identify...Ch. 10 - Prob. 13TFCh. 10 - In order to detect recombinant cells, plasmids...Ch. 10 - Plasmids are the only vectors currently available...Ch. 10 - You are a public health official trying to...Ch. 10 - a.Construct a strand of complementary DNA (cDNA)...Ch. 10 - a.Explain whether or not DNA polymerase from a...Ch. 10 - a.Define the term RFLP. Explain how RFLPs are...Ch. 10 - Prob. 5CTQCh. 10 - From chapter 6, figure 6.19. What has happened to...Ch. 10 - Prob. 2VCCh. 10 - Using the words that follow, please create a...
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- which step of the polymerase chain reaction takes place at 98 degrees?arrow_forwardHow many different temperatures are used in each cycle of the polymerase chain reaction?arrow_forwardwhat are some Limitations/sources of error with suitable rationalization that may occur during the polymerase chain reaction?arrow_forward
- Describe the main steps in using the polymerase chain reaction to amplify a small amount of DNA.arrow_forwardA real-time polymerase chain reaction is a laboratory technique of molecular biology based on the polymerase chain reaction. This technique detects the fluorescence produced by reporter molecules like SYBR® Green dye or TaqMan® probe during the DNA amplification reaction. Compare and contrast between SYBR® Green and TaqMan® probe with illustrations.arrow_forwardWhat do you think are the crucial steps in Polymerase Chain Reaction technique? Enumerate the advantage and disadvantage of Polymerase Chain Reaction technique? What are the things to consider in making a good primer? Does temperature affects the primer design? Discuss briefly.arrow_forward
- The synthetic unit of the polymerase chain reaction is the replica. True or false?arrow_forwardWhat specifically will happen if DNA polymerase is inaccurate during DNA synthesis? Explain how this inaccuracy might affect the organism.arrow_forwardDescribe a typical program used for DNA amplification with the thermal cycler and the purpose of each steps.arrow_forward
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