Microbiology: A Systems Approach
5th Edition
ISBN: 9781259706615
Author: Marjorie Kelly Cowan Professor
Publisher: McGraw-Hill Education
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Chapter 10, Problem 11TF
Summary Introduction
Introduction:
PCR is a technique used to replicate and produce multiple copies of DNA. PCR has a wide application in the field of research, medicine, agriculture, and many other fields.
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The synthetic unit of the polymerase chain reaction is the replica. True or false?
List the fundamental steps of the polymerase chain reaction.
Outline the essential steps in the polymerase chain reaction.
Chapter 10 Solutions
Microbiology: A Systems Approach
Ch. 10.1 - Provide examples of practical applications of...Ch. 10.2 - Prob. 2AYPCh. 10.2 - Describe how gel electrophoresis is used to...Ch. 10.2 - Prob. 4AYPCh. 10.2 - Prob. 5AYPCh. 10.3 - Prob. 6AYPCh. 10.3 - List examples of genetically modified bacteria,...Ch. 10.4 - Prob. 8AYPCh. 10.4 - Prob. 9AYPCh. 10.5 - Outline in general terms the process of DNA...
Ch. 10.5 - Prob. 11AYPCh. 10.5 - Prob. 12AYPCh. 10.5 - Prob. 13AYPCh. 10.6 - Prob. 14AYPCh. 10 - Which of the following is/are not essential to...Ch. 10 - Prob. 2MCQCh. 10 - The function of ligase is to a. rejoin segments of...Ch. 10 - The creation of biological molecules entirely from...Ch. 10 - Which of the following sequences, when combined...Ch. 10 - A region of DNA in a plasmid that is recognized by...Ch. 10 - Prob. 7MCQCh. 10 - Which of the following is a primary participant in...Ch. 10 - Single nucleotide polymorphisms are found in a....Ch. 10 - Microarrays are used to monitor a. the rate of DNA...Ch. 10 - Prob. 11TFCh. 10 - A nucleic acid probe can be used to identify...Ch. 10 - Prob. 13TFCh. 10 - In order to detect recombinant cells, plasmids...Ch. 10 - Plasmids are the only vectors currently available...Ch. 10 - You are a public health official trying to...Ch. 10 - a.Construct a strand of complementary DNA (cDNA)...Ch. 10 - a.Explain whether or not DNA polymerase from a...Ch. 10 - a.Define the term RFLP. Explain how RFLPs are...Ch. 10 - Prob. 5CTQCh. 10 - From chapter 6, figure 6.19. What has happened to...Ch. 10 - Prob. 2VCCh. 10 - Using the words that follow, please create a...
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- Choose the right combination of components required to set up a polymerase chain reaction from the following: Template RNA, two primers, NTPs, and DNA polymerase Template DNA, two primers, dNTPs, and DNA ligase Template DNA, two primers, NTPs, and DNA ligase Template DNA, two primers, dNTPs, and DNA polymerasearrow_forwardWrite the correct order of steps in polymerase chain reaction.arrow_forwardAssume that a cycle of the polymerase chain reaction takes 20 minutes. How many copies of a single DNA fragment will there be after the reaction has been run for six hours?arrow_forward
- How many different temperatures are used in each cycle of the polymerase chain reaction?arrow_forwardUse a drawing to illustrate the principle of DNA gel electrophoresis. (2 marks)-+arrow_forwardWhich of the following steps is NOT part of a typical polymerase chain reaction? Choose an answer below: primer annealing ligation of DNA fragments by DNA ligase primer extension by DNA polymerase heat denaturation of double stranded DNA none of the abovearrow_forward
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