Write the correct order of steps in polymerase chain reaction.

A DNA strand was sequenced using the Sanger method (https://www.youtube.com/watch?v=KTstRrDTmWI). The reaction tube contained the DNA strand, fluorescently labelled dideoxynucleotide triphosphates (ddATP – yellow, ddGTP – green, ddCTP – blue, ddTTP - red), deoxynucleotide triphosphates, DNA polymerase, or its Klenow fragment. Synthesis of DNA is allowed to proceed, and the results are shown on the right: 15 14 13 12 11 10 (a) What is the sequence of the copy and the template strands? (b) If the template strand were in the 5'-3' direction, what will be the sequence of the DNA copy? Nucleotide Length

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Each cycle of the polymerase chain reaction consists of a shift between three different temperatures (94 C, 60 C, and 72 C), explain what occurs at each of those temperatures.

Which of the following steps is NOT part of a typical polymerase chain reaction?   Choose an answer below: primer annealing ligation of DNA fragments by DNA ligase primer extension by DNA polymerase heat denaturation of double stranded DNA none of the above

There are 6 parts to this question: This is a follow up to the prior question regarding the replication of the DNA strand below. The DNA strand is here for your reference and you do not need to do anything with or to it. TC GATATCGG AGCTATAGCC c) what enzyme separated the parental DNA template strands, d) what bonds were broken? e) what enzyme replicates DNA f) before DNA can be replicated/copied, what must be laid down to allow the enzyme in "e" to replicated the DNA (be specific)? g) our DNA is replicated in many "pieces", what enzyme connects these many "pieces" into one continuous DNA strand that becomes the sister chromatid? h) during what specific phase of the cell cycle does this DNA replication process occur? (This should be a review question from last topics we covered).

What proteins are crucial for creating and maintaining DNA replication forks? Choose the best explanation.   Question 2 options:   Helicase creates the replication fork; primase keeps the single strands from closing shut.   Helicase creates the replication fork; single-strand binding proteins keep the single strands from reuniting.   Ligase creates the replication fork; DNA polymerase II keeps the single strands from reuniting.   Helicase creates the replication fork; ligase keeps the single strands from closing shut.

Write the complementary sequence for the following DNA sequence, in order from 3' to 5': Use all capital letters to write the DNA sequence. ▸ View Available Hint(s) 3'- TAGCTTCACTAGATTCGAA -5' 5' - CGATATTGAGCTAAGCTT-3′ Submit Previous Answers × Incorrect; Try Again

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