Microbiology: A Systems Approach
5th Edition
ISBN: 9781259706615
Author: Marjorie Kelly Cowan Professor
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 10.2, Problem 3AYP
Describe how gel electrophoresis is used to analyze DNA.
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What is used to identify the size of a DNA molecule during gel electrophoresis?
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Chapter 10 Solutions
Microbiology: A Systems Approach
Ch. 10.1 - Provide examples of practical applications of...Ch. 10.2 - Prob. 2AYPCh. 10.2 - Describe how gel electrophoresis is used to...Ch. 10.2 - Prob. 4AYPCh. 10.2 - Prob. 5AYPCh. 10.3 - Prob. 6AYPCh. 10.3 - List examples of genetically modified bacteria,...Ch. 10.4 - Prob. 8AYPCh. 10.4 - Prob. 9AYPCh. 10.5 - Outline in general terms the process of DNA...
Ch. 10.5 - Prob. 11AYPCh. 10.5 - Prob. 12AYPCh. 10.5 - Prob. 13AYPCh. 10.6 - Prob. 14AYPCh. 10 - Which of the following is/are not essential to...Ch. 10 - Prob. 2MCQCh. 10 - The function of ligase is to a. rejoin segments of...Ch. 10 - The creation of biological molecules entirely from...Ch. 10 - Which of the following sequences, when combined...Ch. 10 - A region of DNA in a plasmid that is recognized by...Ch. 10 - Prob. 7MCQCh. 10 - Which of the following is a primary participant in...Ch. 10 - Single nucleotide polymorphisms are found in a....Ch. 10 - Microarrays are used to monitor a. the rate of DNA...Ch. 10 - Prob. 11TFCh. 10 - A nucleic acid probe can be used to identify...Ch. 10 - Prob. 13TFCh. 10 - In order to detect recombinant cells, plasmids...Ch. 10 - Plasmids are the only vectors currently available...Ch. 10 - You are a public health official trying to...Ch. 10 - a.Construct a strand of complementary DNA (cDNA)...Ch. 10 - a.Explain whether or not DNA polymerase from a...Ch. 10 - a.Define the term RFLP. Explain how RFLPs are...Ch. 10 - Prob. 5CTQCh. 10 - From chapter 6, figure 6.19. What has happened to...Ch. 10 - Prob. 2VCCh. 10 - Using the words that follow, please create a...
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- Describe the composition of a DNA microarray, and explain how it is used.arrow_forwardHow can site-specific recombination be used in recombinant DNA technology? Explain with an examplearrow_forwardExplain how electrophoresis separates DNA strands. How is a DNA fingerprinting test interpreted?arrow_forward
- b) Describe how DNA is digested by different restriction enzymes c) Describe how gel electrophoresis is used to estimate the size of DNA fragments.arrow_forwardOutline the steps in automated DNA sequencing via the dideoxy method.arrow_forwardWhat information can be gathered by using DNA gel electrophoresis? Describe the steps in the process and explain how and why gel electrophoresis can be used to separate DNA fragments.arrow_forward
- Explain why DNA fragments migrate in a gel electrophoresis. Which fragments migrate farthest: large or small?arrow_forwardGive the purpose of gel electrophoresis system. If larger DNA is loaded in the gel, what will be the movement of such DNA, how about is smaller DNA is loaded into the gel?arrow_forwardState the function of power supply, DNA marker, and ethidium bromide used in the electrophoresis experiment.arrow_forward
- Why amplify DNA using PCR before doing electrophoresis? Why not just take your DNA sample and directly run a gel?arrow_forwarddescribe the Illumina/Solexa method of "massively parallel DNA sequencing"arrow_forwardHow does gel electrophoresis separate DNA fragments of your unknown sample and How will you be able to track the distance travelled by the DNA during electrophoretic run?arrow_forward
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