Gel electrophoresis is a technique used to separate the DNA, RNA, and protein molecules on the basis of their size and charge present on molecules. Gel electrophoresis method implies an electrical charge to separate or isolate macromolecules (DNA, RNA, and protein).

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Chapter 10, Problem 13TF

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Gel electrophoresis is a technique used to separate the DNA, RNA, and protein molecules on the basis of their size and charge present on molecules. Gel electrophoresis method implies an electrical charge to separate or isolate macromolecules (DNA, RNA, and protein).

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A DNA strand was sequenced using the Sanger method (https://www.youtube.com/watch?v=KTstRrDTmWI). The reaction tube contained the DNA strand, fluorescently labelled dideoxynucleotide triphosphates (ddATP – yellow, ddGTP – green, ddCTP – blue, ddTTP - red), deoxynucleotide triphosphates, DNA polymerase, or its Klenow fragment. Synthesis of DNA is allowed to proceed, and the results are shown on the right: 15 14 13 12 11 10 (a) What is the sequence of the copy and the template strands? (b) If the template strand were in the 5'-3' direction, what will be the sequence of the DNA copy? Nucleotide Length

A piece of DNA is cut into four fragments as shown below. A solution containing the four fragments is placed in a single well at the top of an agarose gel. Using the information given below, draw (below the well) how you think the fragments will be aligned on the gel following electrophoresis. Label each fragment with its corresponding letter. Remember, each band on the gel will be the same width, equal to the width of the well at the top of the gel. These should all be in one lane. What is it about the chemistry of DNA that causes it to be uniformly negatively charged?

A piece of DNA is cut into four fragments as shown below. A solution containing the four fragments is placed in a single well at the top of an agarose gel. Using the information given below, draw (below the well) how you think the fragments will be aligned on the gel following electrophoresis. Label each fragment with its corresponding letter. Remember, each band on the gel will be the same width, equal to the width of the well at the top of the gel. These should all be in one lane. What if you had two different DNA fragments that were exactly the same length as measured in base-pairs. Would it be possible to distinguish them using this type of electrophoresis? How would they appear on a gel?

Answer 2

Question

Chapter 10, Problem 13TF

Summary Introduction

Introduction:

Gel electrophoresis is a technique used to separate the DNA, RNA, and protein molecules on the basis of their size and charge present on molecules. Gel electrophoresis method implies an electrical charge to separate or isolate macromolecules (DNA, RNA, and protein).

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Answer 3

Question

Chapter 10, Problem 13TF

Summary Introduction

Introduction:

Gel electrophoresis is a technique used to separate the DNA, RNA, and protein molecules on the basis of their size and charge present on molecules. Gel electrophoresis method implies an electrical charge to separate or isolate macromolecules (DNA, RNA, and protein).

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Answer 4

Question

Chapter 10, Problem 13TF

Summary Introduction

Introduction:

Gel electrophoresis is a technique used to separate the DNA, RNA, and protein molecules on the basis of their size and charge present on molecules. Gel electrophoresis method implies an electrical charge to separate or isolate macromolecules (DNA, RNA, and protein).

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Answer 5

Chapter 10, Problem 13TF

Summary Introduction

Introduction:

Gel electrophoresis is a technique used to separate the DNA, RNA, and protein molecules on the basis of their size and charge present on molecules. Gel electrophoresis method implies an electrical charge to separate or isolate macromolecules (DNA, RNA, and protein).

Answer 6

Chapter 10, Problem 13TF

Summary Introduction

Introduction:

Gel electrophoresis is a technique used to separate the DNA, RNA, and protein molecules on the basis of their size and charge present on molecules. Gel electrophoresis method implies an electrical charge to separate or isolate macromolecules (DNA, RNA, and protein).

Answer 7

Chapter 10, Problem 13TF

Summary Introduction

Introduction:

Gel electrophoresis is a technique used to separate the DNA, RNA, and protein molecules on the basis of their size and charge present on molecules. Gel electrophoresis method implies an electrical charge to separate or isolate macromolecules (DNA, RNA, and protein).

Answer 8

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Answer 11

Ch. 10.1 - Provide examples of practical applications of...Ch. 10.2 - Prob. 2AYP Ch. 10.2 - Describe how gel electrophoresis is used to...Ch. 10.2 - Prob. 4AYP Ch. 10.2 - Prob. 5AYP Ch. 10.3 - Prob. 6AYP Ch. 10.3 - List examples of genetically modified bacteria,...Ch. 10.4 - Prob. 8AYP Ch. 10.4 - Prob. 9AYP Ch. 10.5 - Outline in general terms the process of DNA...

Gel electrophoresis is a technique used to separate the DNA, RNA, and protein molecules on the basis of their size and charge present on molecules. Gel electrophoresis method implies an electrical charge to separate or isolate macromolecules (DNA, RNA, and protein).

Solution Summary: The author explains that gel electrophoresis is a technique used in molecular biology to analyze the DNA fragments.

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Introduction:

Gel electrophoresis is a technique used to separate the DNA, RNA, and protein molecules on the basis of their size and charge present on molecules. Gel electrophoresis method implies an electrical charge to separate or isolate macromolecules (DNA, RNA, and protein).

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Chapter 10 Solutions

Gel electrophoresis is a technique used to separate the DNA, RNA, and protein molecules on the basis of their size and charge present on molecules. Gel electrophoresis method implies an electrical charge to separate or isolate macromolecules (DNA, RNA, and protein).

Solution Summary: The author explains that gel electrophoresis is a technique used in molecular biology to analyze the DNA fragments.

Concept explainers

Videos

Introduction: Gel electrophoresis is a technique used to separate the DNA, RNA, and protein molecules on the basis of their size and charge present on molecules. Gel electrophoresis method implies an electrical charge to separate or isolate macromolecules (DNA, RNA, and protein).

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Chapter 10 Solutions

Introduction:

Gel electrophoresis is a technique used to separate the DNA, RNA, and protein molecules on the basis of their size and charge present on molecules. Gel electrophoresis method implies an electrical charge to separate or isolate macromolecules (DNA, RNA, and protein).