Essentials of Genetics (9th Edition) - Standalone book
Essentials of Genetics (9th Edition) - Standalone book
9th Edition
ISBN: 9780134047799
Author: William S. Klug, Michael R. Cummings, Charlotte A. Spencer, Michael A. Palladino
Publisher: PEARSON
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Chapter 10, Problem 6PDQ
Summary Introduction

To review:

Predicting correct DNA (deoxyribonucleic acid) replication model by analyzing a genetic model of an unknown creature.

Introduction:

DNA replication in all earth organism is a semi-conservative process. That means each DNA strand present in a DNA double helix can serve as a DNA template and new complementary DNA can form. So, after DNA replication two new double helix DNA molecule produce each containing one old parent strand and one newly formed daughter strand.

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Next, before proceeding to problems 6-8, figure out the following terms: • replication fork • replicon • origin of replication The rest is simple math (almost like: Suppose one painter can paint the entire house in 7 hours. How many painters would it take to paint 10 houses in 24 hours?) 9. A particularly short eukaryotic chromosome has a total of 6 x 10° bp and is replicated in 3 minutes. Assuming all origins initiate at the same time, and that all replication forks move at a rate of 10* bp per minute, approximately how many: a) replication forks should be present in this chromosome? b) origins should be present in this chromosome?
3 of 30 Meselson and Stahl conducted experiments to test three prevailing models for DNA replication. They began by growing E. coli for many generations in medium containing 15N. They then transferred the bacteria into medium containing only 14N, and allowed the bacteria to undergo two rounds of DNA replication. After each round of replication, the scientists performed density-gradient centrifugation of the DNA. Identify the banding patterns predicted by each model after the second round of replication? Drag the test tubes to the appropriate locations in the table to show the banding patterns that each model predicts. Test tubes may be used once or not at all. Reset Help Model 14N/15N-- dispersive semiconservative conservative beginning of experiment 14N/14N-- 15N/15N-- 15N/15N- 15N/15N-- 15N/15N-- first 14N/14N-- replication 14N/15N- 14N/15N-- 15N/15N-- 14N/15N-- 15N/15N- second replication 14N/15N 14N/14N-- 14N/15N-
Recall that Meselson and Stahl used heavy nitrogen (N15) and light nitorgen (N14) to determine the mode of DNA replication. If Meselson and Stahl had observed two bands on their gel following only one round of replication, which replication model would have been supported by this observation? conservative model of replication this observation would not have supported any of their models dispersive model of replication semi conservative model of replication O O O O
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