Essentials of Genetics (9th Edition) - Standalone book
9th Edition
ISBN: 9780134047799
Author: William S. Klug, Michael R. Cummings, Charlotte A. Spencer, Michael A. Palladino
Publisher: PEARSON
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Chapter 10, Problem 10PDQ
Summary Introduction
To review:
The process by which new nucleotides are added to the 3’-OH end of the growing DNA (deoxyribonucleic acid) strand.
Introduction:
DNA polymerase is responsible for
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Kornberg showed that nucleotides are added to the 3' end of eachgrowing DNA strand. In what way does an exposed 3'-OH groupparticipate in strand elongation?
How are deoxyribonucleoside triphosphates (precursors of daughter DNA strands) and ribonucleoside triphosphates (precursors of RNA strand) differ from each other?
Considering that DNA is synthesized in the 5' to 3' direction, which direction must DNA polymerase travel along the template strand: 5' to 3' or 3' to 5' ?
Chapter 10 Solutions
Essentials of Genetics (9th Edition) - Standalone book
Ch. 10 -
CASE STUDY | At loose ends
A researcher was...Ch. 10 - Prob. 2CSCh. 10 - Prob. 3CSCh. 10 - Prob. 4CSCh. 10 - HOW DO WE KNOW? In this chapter, we focused on how...Ch. 10 - Review the Chapter Concepts list on p. 180. These...Ch. 10 - Compare conservative, semiconservative, and...Ch. 10 - Prob. 4PDQCh. 10 - Predict the results of the experiment by Taylor,...Ch. 10 - Prob. 6PDQ
Ch. 10 - Prob. 7PDQCh. 10 - Prob. 8PDQCh. 10 - Prob. 9PDQCh. 10 - Prob. 10PDQCh. 10 - Prob. 11PDQCh. 10 - Prob. 12PDQCh. 10 - Prob. 13PDQCh. 10 -
14. Distinguish between (a) unidirectional and...Ch. 10 - Prob. 15PDQCh. 10 - Define and indicate the significance of (a)...Ch. 10 - Outline the current model for DNA synthesis.Ch. 10 - Why is DNA synthesis expected to be more complex...Ch. 10 - Prob. 19PDQCh. 10 - Several temperature-sensitive mutant strains of E....Ch. 10 - Prob. 21PDQCh. 10 - Prob. 22PDQ
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- Why do higher salt concentrations stabilize the DNA double helix? Or What aspect of the structure of double-stranded DNA destabilizes the helix in the absence of salt?arrow_forwardA solution contains DNA polymerase and the Mg ²+ salts of dATP, dGTP, dCTP, and TTP. The following DNA molecules are added to aliquots of this solution. Which of them would lead to DNA synthesis? (a) A single-stranded closed circle containing 1000 nucleotide units. (b) A double-stranded closed circle containing 1000 nucleotide pairs. (c) A single-stranded closed circle of 1000 nucleotides base-paired to a linear strand of 500 nucleotides with a free 3' -OH terminus. (d) A double-stranded linear molecule of 1000 nucleotide pairs with a free 3’-OH group at each end.arrow_forwardWrite the sequence of the complementary DNA strand that pairs with each of the following DNA base sequences:(a) TTAGCC(b) AGACATarrow_forward
- If the DNA sequence A-T-T-G-G-C-C-T-A on an informational strand mutated and became A-C-T-G-G-C-C-T-A, what effect would the mutation have on the sequence of the protein produced?arrow_forwardThe base analog 5-bromouracil undergoes tautomerization. One tautomer behaves like thymine, and the other behaves like cytosine. If 5-bromouracil is incorporated in the DNA, what is the minimum number of cycle(s) of DNA replication that must occur to mutate the codon for proline (CCC) into the codon for serine (TCC)? A) one cycle B) two cycles C) three cycles D) 5-bromouracil cannot induce this mutationarrow_forwardThe base analog 5-bromouracil (5BU), which sterically resembles thymine, more readily undergoes tautomerization from its keto form to its enol form than does thymine. 5BU can be incorporated into newly synthesized DNA when it pairs with adenine on the template strand. However, the enol form of 5BU pairs with guanine rather than adenine. (a) Draw the 5BU · G base pair. (b) What type of mutation results?arrow_forward
- What is the nucleotide sequence of the DNA strand that is complementary to 5'-GGCGCAACTGTCACAA-3'?arrow_forwardDNA molecules of different sizes are often separated with the use of a technique called electrophoresis . With this technique, DNA molecules are placed in a gel, an electrical current is applied to the gel, and the DNA molecules migrate toward the positive (+) pole of the current. What aspect of its structure causes a DNA molecule to migrate toward the positive pole?arrow_forwardThe base composition for one of the strands of a DNA double helix is 19% A, 34% C, 28% G, and 19% T. What is the percent base composition for the other strand of the DNA double helix?arrow_forward
- Do any strands of nucleic acid exist in nature in which part of the strand is DNA and another part of that same strand is RNA? If so, describe when such strands of nucleic acid are synthesized. Is the RNA component at the 5′ end or at the 3′ end?arrow_forwardWhat is the total number of hydrogen bonds that exist between the DNA strand 5’-TTCAGAG-3’ and its complementary strand?arrow_forwardInsulin is synthesized as preproinsulin, which has 81 amino acids. How many heterocyclic bases must be present in the informational DNA strand to code for preproinsulin (assuming no introns are present)?arrow_forward
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