Essentials of Genetics (9th Edition) - Standalone book
9th Edition
ISBN: 9780134047799
Author: William S. Klug, Michael R. Cummings, Charlotte A. Spencer, Michael A. Palladino
Publisher: PEARSON
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Chapter 10, Problem 19PDQ
Summary Introduction
To review:
The perception that if two different microorganisms possess similar base composition in their DNA, are the two organisms closely identical or not.
Introduction:
DNA is composed of some molecules called as nucleotides. Each
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With regard to Chargaff’s experiment described in Figure shown,answer the following:A. What is the purpose of paper chromatography?B. Explain why it is necessary to remove the bases in order todetermine the base composition of DNA.C. Would Chargaff’s experiments have been convincing if theyhad been done on DNA from only one species? Discuss.
Much of the human genome consists of repetitious DNA. Describe the difference between microsatellite and minisatel lite DNA. How is this repetitious DNA useful for identifying individuals by the technique of DNA fingerprinting?
In isolating DNA in strawberries, can you isolate other biomolecules aside from DNA just by using commonly available household materials and chemicals? Elaborate on your answer.
Chapter 10 Solutions
Essentials of Genetics (9th Edition) - Standalone book
Ch. 10 -
CASE STUDY | At loose ends
A researcher was...Ch. 10 - Prob. 2CSCh. 10 - Prob. 3CSCh. 10 - Prob. 4CSCh. 10 - HOW DO WE KNOW? In this chapter, we focused on how...Ch. 10 - Review the Chapter Concepts list on p. 180. These...Ch. 10 - Compare conservative, semiconservative, and...Ch. 10 - Prob. 4PDQCh. 10 - Predict the results of the experiment by Taylor,...Ch. 10 - Prob. 6PDQ
Ch. 10 - Prob. 7PDQCh. 10 - Prob. 8PDQCh. 10 - Prob. 9PDQCh. 10 - Prob. 10PDQCh. 10 - Prob. 11PDQCh. 10 - Prob. 12PDQCh. 10 - Prob. 13PDQCh. 10 -
14. Distinguish between (a) unidirectional and...Ch. 10 - Prob. 15PDQCh. 10 - Define and indicate the significance of (a)...Ch. 10 - Outline the current model for DNA synthesis.Ch. 10 - Why is DNA synthesis expected to be more complex...Ch. 10 - Prob. 19PDQCh. 10 - Several temperature-sensitive mutant strains of E....Ch. 10 - Prob. 21PDQCh. 10 - Prob. 22PDQ
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- Could actual fi ngerprints taken from human fi ngers be used to perform a DNA fi ngerprint? Explain.arrow_forward3) Us the image below to complete questions A-C I Marker 10,000 8,000 5,000 2,000 1,000 500 Control EcoRI BamHI Hind IIIarrow_forwardIf DNA is extracted from the juvenile leaves of mint plant. Then how to check the quantity and quality of the extracted DNA through gel electrophoresis. How to prepare 1% agarose gel for this purpose?arrow_forward
- Given the electrophoresis profile of a Sanger sequencing result, what was the sequence of the original DNA sample used for sequencing? GGTAACC CCAATGG GGTTACC CCATTGGarrow_forwardAmplified target regions of four different samples were separated using gel electrophoresis. DNA fragments labeled with the isotope P32 were separated by gel electrophoresis. P32 was used to a) label fragments for imaging. b) distinguish between the fragment’s 3’ and 5’. c) observe the location of the fragments. d)speed up the rate of separation by electrophoresisarrow_forwardWith regard to DNA microarrays, answer the followingquestions:A. What is attached to the slide? Be specific about the numberof spots, the lengths of DNA fragments, and the origin ofthe DNA fragments.B. What is hybridized to the microarray?C. How is hybridization detected?arrow_forward
- What are the benefits of using a mixture classification scheme as outlined in DNA Box 14.1? What would be the advantages of using software for deciphering mixture components?arrow_forwardWhat is DNA? Provide a 5-sentence long description only.arrow_forwardHow could you explain the variable DNA concentrations obtained between the different student samples 1-4 in the table above? 2.Which student sample(s) potentially contained proteins, explain how you deduced this from the result? How could you explain the variable DNA concentrations obtained between the different student samples 1-4 in the table above? 2.Which student sample(s) potentially contained proteins, explain how you deduced this from the result?arrow_forward
- In DNA-DNA Hybridization, sequences from two separate organisms that show partial hybridization suggest _______. a) They are related b) suggest they are not related c)suggest that they are the same organismarrow_forwardYou have isolated genomic DNA and plasmid DNA from a bacterial culture. Please answer the following questions: 1. What solution did you use to elute DNA from the silica resin column? 2. What is the function of the chaotropic salt in DNA isolation? 3. Why do we add the chaotropic salt for genomic DNA before lysis and for plasmid isolation we add the salts after the lysis step?arrow_forwardExplain the principles behind DNA isolation using silica membrane spin column from Qiagen. 2 After DNA isolation using Qiagen extraction kit, the final flow through was subjected to spectrophotometric measurements. Explain the situation if the absorbances were found to be: i) The absorbance at 260 nm is higher than absorbance at 280 nm? ii) The absorbance at 230 is higher than absorbance at 260 nm?arrow_forward
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