Genetic Analysis: An Integrated Approach (3rd Edition)
Genetic Analysis: An Integrated Approach (3rd Edition)
3rd Edition
ISBN: 9780134605173
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
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Chapter 10, Problem 5P

Human late prophase karyotypes have about 2000 visible G bands. The human genome contains approximately 22,000 genes. Consider the region 5p1 .5 through the end of the short arm of chromosome in figure 10 .5 , and assume the entire region is deleted. Approximately how many genes will be lostas a result of the deletion?

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An STR on chromosome 5 is very closely linked to a gene involved in a dominant metabolism disorder (one one copy of the mutation causes the disease). There are four different alleles of the STR (Q, B, S, and L), each with different numbers of GATCTCG repeats. The S allele has 2 repeats, the B allele has 7 repeats, the Q allele has 10 repeats, and the L allele has 5 repeats. Part 1 Lane 1 of the gel shown below indicates the locations of PCR products corresponding to all four STR alleles when run together in one lane. DNA was loaded into the wells at the top of the gel. Move the letter of each allele to its correct position at the left of Lane 1 to indicate where each allele runs on the gel. 0000 Gabriel Andre Lane 1 O Maria Andre Maria Juliana B L Part 2 Andre and Maria are the parents in this family; all others shown on the gel are children. Daughter Juliana is born with the metabolism disorder, but daughter Camila does not have the disorder. Assuming no recombination between the STR…
To detect the CAG repeat expansion with a particular gene where 30 repeats in Normal changes to 250 repeats in a certain disease, how can we diagnose the condition.  How To identify Y chromosome microdeletion ( which involves the deletion of AZF locus) using conventional karyotyping? If not then why. How will you diagnose a chromosomal translocation event?
The fruit fly Drosophila melanogaster has about 2 x 10^8 base pairs of DNA per haploid genome, of which about 75% in nonrepeated DNA. The DNA is distributed among four pairs of homologous chromosomes, which have a total of about 5,000 visible bands when in polytene forms in the salivary gland. The number of genes initially estimated from mutational studies was also about 5,000 but recent DNA sequencing studies suggest that the gene number may be somewhat higher. a. Why was it tempting to speculate that each band corresponds to a single gene? What does this suggest about the number of different proteins Drosophila can make? Does that seem like a reasonable number to you? b. Assuming all the nonrepeated DNA is uniformly distributed in the chromosomes, how much nonrepeated DNA (in base pairs) is there in average band?

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Genetic Analysis: An Integrated Approach (3rd Edition)

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