We want to extract DNA from this culture of bacteria suspended in Media the bacteria cells are floating in the liquid. Our first step is to centrifuge our sample. Do we keep the supernatant or the pellet and why? A. Supernatant because it contains Calais or nutrients B. Pellet because it contained only DNS and the supernatant contains the rest of the cell part C. Supernatant because it contains only dna and the pellet contains the rest of the cell parts D. Pellet because it contains the parts of the bacterial cell with the plasmid DNA

We want to extract DNA from this culture of bacteria suspended in Media the bacteria cells are floating in the liquid. Our first step is to centrifuge our sample. Do we keep the supernatant or the pellet and why? A. Supernatant because it contains Calais or nutrients B. Pellet because it contained only DNS and the supernatant contains the rest of the cell part C. Supernatant because it contains only dna and the pellet contains the rest of the cell parts D. Pellet because it contains the parts of the bacterial cell with the plasmid DNA

Essentials of Pharmacology for Health Professions

7th Edition

ISBN:9781305441620

Author:WOODROW

Publisher:WOODROW

Chapter14: Antineoplastic Drugs

Section: Chapter Questions

Problem 11CRQ

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The figure below illustrates the stages of production of useful medical products using recombinant DNA technology. Closely study the figure then answer the Activity 1 questions that follow: Human cell Bacterium 1. DNA Plasmid DNA Human insulin-producing gone 2. DNA is cut with restriction Bacterial DNA with human gone inserted enzymes. 3. Plasmid is reintroduced into bacterium. 4. Engineered bacteria multiply producing insulin. 5. Insulin is Human insulin separated and purified to produce human insulin. 6. Insulin injected into patient 1. What is a plasmid? Make a diagram of bacterial cell containing a plasmid. 2. How do you explain the selection of plasmids for carrying the desired gener 3. Follow the steps of human insulin production, then 4. Make a diagram for the production of growth hormone. first
The figure below illustrates the stages of production of useful medical products using recombinant DNA technology. Closely study the figure then answer the Activity 1 questions that follow: Human cell Bacterium 1. DNA Plasmid DNA Human insulin-producing gone 2. DNA is cut with restriction Bacterial DNA with human gone inserted enzymes. 3. Plasmid is reintroduced into bacterium. 4. Engineered bacteria multiply producing insulin. 5. Insulin is separated and purified to produce human insulin. Human insulin Itulin 6. Insulin injected into patient 1. What is a plasmid? Make a diagram of bacterial cell containing a plasmid. 2. How do you explain the selection of plasmids for carrying the desired gener 3. Follow the steps of human insulin production, then 4. Make a diagram for the production of growth hormone. first
Which of the following objects would you use microinjection in order to transfer DNA into? a. Egg cell visible to the human eye b. Animal virions c. Escherichia coli that has an F plasmid d. Bacteria that has been heat shocked
Choose the one answer that fits best. The pGLO plasmid you used contains an araC gene, a GFP gene and a bla gene. If you performed the exact same experiment with a plasmid that contained a GFP gene and a bla gene, but was missing the araC gene, what would you expect to see on your four plates? a. The positive control would not have any bacterial lawn b. The negative control would have as many colonies as the experimental plate c. The LB/Amp/Ara plate would have some colonies but they would not be fluorescent under UV light d. The experimental plate would not have any colonies e. The LB/Amp plate containing E. coli (+)pGLO would be fluorescent
Match the outcomes on the left with the laboratory steps on the right. ___ Harvest the cells A. Add salt ___ Dissolve cell membranes B. Add protease, incubate at 50°C ___ Precipitate the DNA C. Mix in a detergent solution ___ Break down proteins D. Layer cold alcohol over cell extract ___ Make DNA less soluble in water E. Gently chew the insides of your mouth, then rinse vigorously with water
The following image is of an agarose gel. If DNA samples were loaded to this gel and the electrophoresis experiment was started, explain what would happen and why.
Examine the pGLO plasmid DNA solution with the UV lamp. Note your observations. Using a micropipettor, withdraw 10 ul of plasmid and mix it into the cell suspension of the +PGLO tube by pipetting up and down three times. Close the tube and return it to the rack on ice. Also close the -PGLO tube. Do not add plasmid DNA to the -pGLO tube. Why not?
Choose the one answer that fits best. Which of the following statements regarding bacteria is NOT correct? a. During conjugation, two bacteria exchange genetic information directly via pili b. Bacterial populations have a higher mutation rate because their DNA Polymerases do not have proofreading capability c. Bacterial growth by binary fission is exponential and each division usually takes only a couple of hour or less d. Plasmids carry just a few genes and are replicated independently from the bacterial chromosome e. Cocci that are arranged in clusters/bunches are called staphylococci
The presence of colonies on the bacterial plate post transformation suggests which of the following? Group of answer choices The colonies contain the transformed plasmid and are not resistant to the antibiotic The colonies contain the transformed plasmid and are resistant to the antibiotic The colonies do not contain the transformed plasmid and are not resistant to the antibiotic The colonies do not contain the transformed plasmid and are resistant to the antibiotic
_1. Bacterial proteins that have the ability to cut both strands of the DNA molecule at certain points 2. Contain foreign DNA 3. Is made by connecting segments of DNA from different sources _ 4. General term for a carrier used to transfer a foreign DNA fragment into a host cell 5. A small ring of DŇA found in a bacterial cell 6. The procedure for cleaving DNA from an organism into small segments, and inserting the f. genetic engineering or segments into another organism a. recombinant DNA b. vector c. restriction enzymes d. plasmid e. transgenic organisms recombinant DNA technology
Use the figure of the gel below to answer the following questions. Controls 1 2 3 Experimentals 4 5 6 7 8 8. Identify which well contains the DNA ladder-Helps identify unknown DNA to which individual. 9. Identify which well contains a smear-Contamination of the well or degrading the DNA 10. Identify which well contains primer dimer. 11. Identify which well shows results similar to the positive control.
The other options are: a. RNA cannot be digested by restriction enzymes b. RNA is small enough to be resolved on an agarose gel without the need for restriction digestion. c. RNA is single stranded and DNA is double stranded